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Roles of p53 and ras gene mutations in genesis of oral SCC in India

Research Project

Project/Area Number 06044072
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TSUCHIDA Nobuo  Faculty of Dentistry, Tokyo Medical and Dental University Professor, 歯学部, 教授 (60089951)

Co-Investigator(Kenkyū-buntansha) SAITO Yuichi  Faculty of Dentistry, Tokyo Medical and Dental university, 歯学部, 助教授 (20013930)
MUNIRAJAN A.K.  School of Biological Sciences, Madurai Kamaraj University, 生物科学部, 特別研究員
SHANMUGAM G.  School of Biological Sciences, Madurai Kamaraj University, 生物科学部, 教授
A.K Muniraja  Madurai Kamaraji大学, 生物科学部, 特別研究員
G Shanmugam  Madurai Kamaraji大学, 生物科学部, 教授
石井 裕子  東京医科歯科大学, 歯学部, 教務職員 (00251546)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsoral cancer / squamous cell carcionoma / p53 gene / ras gene / HPV / PCR / SSCP / p53 / 癌抑制遺伝子 / ras / タバコ
Research Abstract

In order to find differences of the genesis of oral squamous cell carcinomas in between India and Japan, 53 oral tumor samples obtained from Government Rajaji Hospital, Madurai were analyzed for p53 and ras mutations and another 50 uterine cervical cancer samples from Anna Cancer Hospital, Kancheepuram for p53 and HPV status. DNA was isolated form these tumors and specific regions were PCR-amplified using primers for p53, ras and HPV.The amplifications were done in the presence of 32PdCTP and the products for p53 and ras were screened for mutations by SSCP analysis. SSCP positive samples for p53 were confirmed and sequenced in Tokyo Medical Dental University by A.K.Munirajan who visitied here thrice for this purpose and to learn other techniques. The results indicated p53 mutations in 11 out of 53 (21%) oral samples. Most of these mutations were transition, which is consistent with the involvement of tabacco-related nitrosoamines in the etiology of oral squamous cell carcinoma. Ras mutations were relatively of high frequency (32%). However the p53 and ras mutations did not overlap in a sample. Moreover, most ras mutations were detected in H-ras gene. Unider conditions which detected HPV in 60-70% of cervical cancers we were unable to demosntrate the presence of HPV in the oral cancers, suggesting no significant role of this verus in genesis of Indian oral squamous cell carcinoma.

Report

(2 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 1.Hirano,Y.,Yamato.K.,and Tsuchida,N.: "A tempertature sensitive mutant of the human p53,val138,arrest rat cell growth without induced expression of cip 1/waf1/sdi1 after temperature shit-down." Oncogene.10. 1879-1885 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 2.Yamato,K.,Yamanoto,M.,Hirano Y.,and Tsuchida,N.: "A human temprature-sensitive p53 mutatnt,p53val-138 ; Modulation of the cell cycle,viability and expression of p53-responsive genes." Oncogene. 11. 1-6 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 3.Hirano,Y.,Tsutsumi-Ishii,Y.,and Tsuchida,N.: "Roles of p53 mutation in cell line establihsment and identification of the minimum transactivation and transform suppression domains." Eur.J.Cancer. 32B. 129-135 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 4)Tsutsumi-Ishii,Y.,Tadokoro,K.Hanaoka,F.,and Tsuchida,N.: "Response of heat shock element (HSE) within the human HSP70 promoter to mutated p53 genes." Cell Growth & Differ.6. 1-8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 5)Munirajan,A.K.,Tsutsumi-Ishii,Y.,Mohanprasad,K.C.,Hirano,Y.,Munakata,N.,Shanmugam,G.,and Tsuchida,N: "p53 gene mutations in oral carcinomas from India." Int.J.Cancer in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 6)Takada,S.,Tsuchida,N.,Kobayashi,M.and Koike,K.,: "Disruption of the function of tumor-suppressor gene p53 by the hepatitis B virus X protein and hepatocarcinogenesis." J.Cancer Res.Clin.Oncol.,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 7)Sekiguchi,T.,Nakashima T.,Hayashida,T.,Kuraoka,A.,Hashimoto,S.,Tsuchida,N.,Shibata,Y.,Hunter,T.,and Nishimoto,T.: "Apoptosis is induced in BHK cells by the tsBN462/13 mutation in the CCG1/TAFII250 subunit of the TFIID basal transcription factor." Exp.Cell.Res.,. 218. 161-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 8)Sugito,K.,Yamane,M.,Hattori,H.,Tohnai,I.,Ueda,M.,Tsuchida,N.,and Ohtsuka,K.: "Interaction between hsp70 and hsp40,eucaryotic homologues of DnaK and DnaJ,in human cells expressing mutant-type p53." FEBS Letters. 358. 161-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirano, Y., Yamato, K., and Tsuchida, N.: "A tempertature sensitive mutant of the human p53, val138, arrest rat cell growth without induced expression of cip1/waf1/sdi1 after temperature shit-down." Oncogene. 10. 187.9-188.5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamato, K., Yamanoto, M., Hirano Y., and Tsuchida, N.: "A human temprature-sensitive p53 mutatnt, p53val-138 ; Modulation of the cell cycle, viability and expression of p53-responsive genes." Oncogene. 11. 1-6 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirano, Y., Tsutsumi-Ishii, Y., and Tsuchida, N.: "Roles of p53 mutation in cell line establihsment and identification of the minimum transactivation and transform suppression domains." Eur.J.Cancer. 32B. 129-135 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi-Ishii, Y., Tadokoro, K., Hanaoka, F., and Tsuchida, N.: "Response of heat shock element (HSE) within the human HSP70 promoter to mutated p53 genes." Cell Growth & Differ.6. 1-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Munirajan, A.K., Tsutsumi-Ishii, Y., Mohanprasad, K.C., Hirano, Y., Munakata, N., Shanmugam, G., and Tsuchida, N.: "P53 gene mutations in oral carcinomas from India." Int.J.Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takada, S., Tsuchida, N., Kobayashi, M.and Koike, K.: "Disruption of the function of tumor-suppressor gene p53 by the hepatitis B virus X protein and hepatocarcinogenesis." J.Cancer Res.Clin.Oncol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sekiguchi, T., Nakashima T., Hayashida, T., Kuraoka, A., Hashimoto, S., Tsuchida, N., Shibata, Y., Hunter, T., and Nishimoto, T.: "Apoptosis is induced in BHK cells by the tsBN462/13 mutation in the CCG1/TAFll250 subunit of the TFllD basal transcription factor." Exp.Cell.Res.218. 161-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugito, K., Yamane, M., Hattori, H., Tohnai, I., Ueda, M., Tsuchida, N., and Ohtsuka, K.: "Interaction between hsp70 and hsp40, eucaryotic homologues of DnaK and DnaJ,in human cells expressing mutant-type p53." FEBS Letters. 358. 161-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi-Ishii Y,Tadokoro K,Hanaoka F,and Tsuchida N: "Response of heat shock element whithin the human HSP70 promoter to mutated p53 genes" Cell Growth & Differ.6. 1-8 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hirano Y,Tsutsumi-Ishii Y,and Tsuchida N: "Roles of the p53 mutation in cell line establishment and identification of the minimum trans-activation and transform suppression domains." Eur.J.Cancer B.Oral Oncology. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hirano Y,Yamato K,and Tsuchida N: "A temperature sensitive mutant of the human p53,Vall38,arrests rat cell growth without induced expression of cip1/waf1/sdi1 after temperature shift-down" Oncogene. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sugito K,Yamane M,Hattori H,Hayashi Y,Tohnai I,Ueda M,Tsuchida N,and Ohtsuka K: "Interaction between hsp70 and hsp40,eukaryotic homologues of DnaK and DnaJ,in human cells expressing mutant-type p53" FEBS Letters. 358. 161-164 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Munirajan A.K,Tsutsunmi-Ishii Y,Mohanprasad R.K.C,Hirano Y,Munakata N,Shanmugam G,and Tsuchida N: "p53 geen mutation in oral carcinomas from India" (投稿準備中).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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