| Project/Area Number |
06044073
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NODA Masaki Medical Research Institule, Tokyo Medical and Dental University, Professor, 難治疾患研究所, 教授 (50231725)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAGUTI Nanako Tokyo Medical and Dental University, 難治疾患研究所, 助手 (10200700)
DENHARDT David Rutgers University, ネルソン生物学研究所, 所長
PETER Koopma クイーンズランド大学, 教授
BRUCE Spiege ダナファーバー研究所, 教授
ERIC Olson テキサス大学, MDAndersor癌研究所, 教授
SUSAR Rittli ラトガース大学, ネルソン生物学研究所, 助教授
DAYID Denhar ラトガース大学, ネルソン生物学研究所, 所長
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1995: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1994: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | Osteopontin / Osteoblat / Bone Morphogenetic Protein / Bone Formation / Bone Protein / Osteoclast / gene expression / Differentiation / osteopontin / osteoblat / osteoclast |
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| Research Abstract |
To eluidate the role of osteopontinm, we prepared to examine the effect of osteopontin overexpression as well as inactivation of osteopontin gene in mice system. We first cloned murine osteopontin gene and constructed vectors by using the upstream exons. These constructs also contained neo-gene cassett and were introduced into murine ES cells by electroporation. ES cells containing homologous recombination in the osteopontin gene were selected and they were introduced into the blastocele. Chymeric mice were identified by their coat color and were mated to prodece mice which lack osteopontin gene through homolo gous recombination. With regard to over expression experiments, osteopontin gene was cloned down stream of a strong promoter to yield inducible expression vectors and this vector was introduced via microinjection into fertilized eggs which were subsequently implanted into the uterus of pseudo-pregnant mice.
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