• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Joint Study on Structure of Polymers by New Solid State NMR

Research Project

Project/Area Number 06044075
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

ASAKURA Tetsuo  Tokyo University of Agriculture and Technology Professor, 工学部, 教授 (30139208)

Co-Investigator(Kenkyū-buntansha) 出村 誠  東京農工大学, 機器分析センター, 講師 (70188704)
GULLION T  Department of Chemistry Florida State University, Florida State Un, Assistant
NICHOLSON L.K  Center for Advanced Technology Cornnel University, Cornnel U, Assistant
CROSS T.A  National Magnetic Resonance Facility Florida State University, Flo, Professor
石原 邦  東京農工大学, 農学部, 教授 (70014925)
棚沢 一朗  東京農工大学, 工学部, 教授 (30013105)
松田 浩珍  東京農工大学, 農学部, 助教授 (80145820)
永井 正敏  東京農工大学, 大学院生物システム応用科学研究科部, 助教授 (20111635)
松岡 秀明  東京農工大学, 工学部, 教授
NICHOLOSON L  Cornell University, Assistant
GULLION Terr  Department of Chemistry, Florida State Un, Assistant
CROSS Timoth  National Magnetic Resonance Facility, Flo, Professor
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsSolid State NMR / Silk / Isotope Labelling / Angular-dependent NMR / REDOR / NMR Structural Analysis / Amorphous Structure / Dynamics / Chemical shift Anisotropy / CROWN / スピン拡散
Research Abstract

The objective of this international joint study is development of novel analytical methods using angular-and distance-dependent theory of solid state NMR in atomic level and its application to structural analysis of polymers in atomic level. Orientation-dependent NMR interactions such as dipole-dipole, quadrupole and chemical shielding yield structural information on polymers in the solid state. If these parameters are observed for each site in oriented polymers, the solid state NMR methods can give even the atomic co-ordinates of the polymers. This is especially effective in the determination of the atomic co-ordinates of uniaxially oriented peptides or proteins in which the X-ray technique gives only little structural information. This analytical method could be applied to obtain structural information of oriented polyamide fibers such as poly (p-phenylene terephthalamide) (PPTA), and poly (m-phenylene isophthalamide) (PMIA) and poly (4-methyl-m-phenylene terephthalamide) (P4M-MPTA) fibers without isotope labelling of the samples. The oriented carbonyl carbon labelled poly (ethylene terephthalate) (PET) films are also analyzed with this method. Especially, more quantitative structural information was obtained for a locally ordered domain which has been recognized as an amorphous domain in X-ray diffraction analysis in heterogeneous polymer samples. The REDOR (Rotational Echo Double Resonance) technique has been applied to structure determination of a silk fibroin model compound. In general, this does not require the orientation of the samples in the analysis, but selective isotope labelling between specified nuclear pairs in the samples is required which becomes frequently to be a problem.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] T.Asakura et al.: "Structural Analysis of Uniaxially Orineted ^<13>C-labelled Poly(ethylene terephthalate)Films Studied with Solid-State ^<13>C Nuclear Magnetic Resonance Spectroscopy." Polymer. 37. 1965-1973 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Nishiyama et al.: "The effect of pH of N-methacryloyl glycine primer on bond strength to acid-etched dentin" J.Biomed.Mater.Res.31. 379-384 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Uchiyama et al.: "Design and Synthesis of C-Linked Fucosides ad Inhibitors of E-Selectin" Bioorganic & Medicinal Chemistry. 4. 1149-1165 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Asakura et al.: "NMR Study on Silk I Structure of Bombyx mori Silk Fibroin with ^<15>N- and ^<13>C-NMR Chemical Shift Contour Plots" Biopolymers. 41. 193-203 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Asakura et al.: "^2H-Labelling of Silk Fibroin Fibers and Their Structural Characterization by Solid State ^2H-NMR" Macromolecules. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Demura et al.: "Structure of Uniaxially Aligned ^<13>C Labeled Silk Fibroin Fibers with Solid State ^<13>C-NMR" J.Molecular Structure. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Matsuzaki et al.: "NMR Spectroscopy and Stereoregularity of Polymers" Japan Scientific Societies Press, 277 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Asakura et al.: "Annual Reports on NMR Spectrocopy(Ed.G.A.Webb)" ACADEMIC PRESS,London(in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Asakura et al.: "Structural Analysis of Uniaxially Orineted ^<13>C-labelled Poly (ethylene terephthalate) Films Studied with Solid-State ^<13>C Nuclear Magnetic Resonance Spectroscopy." Polymer. 37. 1965-1973 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Nishiyama et al.: "The effect of pH of N-methacryloyl glycine primer on bond strength to acid-etched dentin" Biomed.Mater.Res.31. 379-384 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Uchiyama et al.: "Design and Synthesis of C-Linked fucosides as Inhibitors of E-Selectin" Bioorganic & Medicinal Chemistry. 4. 1149-1165 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Asakura et al.: "NMR Study on Silk I Structure of Bombyx mori Silk Fibroin with ^<15>N-and ^<13>C-NMR Chemical Shift Contour Plots" Biopolymers. 41. 193-203 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Asakura et al.: "^2H-Labelling of Silk Fibroin Fibers and Their Structural Characterization by Solid State ^2H-NMR" Macromolecules. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Demura et al.: "Structure of Uniaxially Aligned ^<13>C Labeled Silk Fibroin Fibers with Solid State ^<13>C-NMR" J.Molecura Structure. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Matsuzaki et al.: Japan Scientific Societies Press. NMR Spectroscopy and Stereoregularity of Polymers, 277 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Asakura et al.: ACADEMIC PRESS,London (in press). Annual Reports on NMR Spectroscopy, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Asakura et al.: "Structural Analysis of Uniaxally Orineted ^<13>C-labelled Poly(ethylene terephthalate) Films Studied with Solid-State ^<13>C Nuclear Magnetic Resonance Spectroscopy." Polymer. 37. 1965-1973 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Nishiyama et al.: "The effect of pH of N-methacryloyl glycine primer on bond strength to acid-etched dentin" J.Biomed.Mater.Res.31. 379-384 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Uchiyama et al.: "Design and Synthesis of C-Linked Fucosides as Inhibitors of E-Selectin" Bioorganic & Medicinal Chemistry. 4. 1149-1165 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Asakura et al.: "NMR Study on Silk I Structure of Bombyx mori Silk Fibroin with ^<15>N- and ^<13>C-NMR Chemical Shift Contour Plots" Biopolymers. 41. 193-203 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Asakura et al.: "^2H-Labelling of Silk Fibroin Fibers and Their Structural Characterization by Solid State ^2H-NMR" Macromolecules. in press. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Demura et al.: "Structure of Uniaxially Aligned ^<13>C Labeled Silk Fibroin Fibers with Solid State ^<13>C-NMR" J.Molecular Structure. in press. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Matsuzaki et al.: "NMR Spectroscopy and Stereoregularity of Polymers" Japan Scientific Societies Press, 277 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Asakura et al.: "Annual Reports on NMR Spectroscopy(Ed.G.A.Webb)" ACADEMIC PRESS,London(in press), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 朝倉哲郎: "角度依存固体NMRによるタンパク質繊維の原子座標の決定" 生物物理. 35. 280-282 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tetsuo Asakura: "High-Resolution Solid State ^<13>CNMR Spectroscopy of Syndiotactic Polypropylene" Polymer J.28. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Norihiro Nishiyama: "Effects of a structural change in collagen upon binding to conditioned dentin studied by ^<13>CNMR" Journal of Biomedical Materials Research. 29. 107-111 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tetsuo Asakura: "The Relationship between Amide Proton Chemical Shifts and Secondary Structure in proteins" J.Biomol.NMR. 6. 227-236 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimiko Umemoto: "Conformations of Synthetic Model Peptides for Plasmodium falciparum Circumsporozoite Protein in Me_2SO by ^1HNMR and Distance Geometry Calculations" Polymer Journal. 27. 347-360 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Michael P.Williamson: "Application of ^1HNMR chemical shifts to measure the quality of protein structures" J. Mol. Biol. 247. 541-546 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 朝倉哲郎: "新繊維科学-ニューフロンティアへの挑戦-" 通商産業調査会, 5 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 朝倉哲郎: "合成高分子「構造解析」“新高分子実験学5巻「高分子の構造(1)-磁気共鳴」"(安藤勲編)" 共立出版, 32 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tetsuo Asakura: "Polymers: Regio-Irregular Structure in “Encyclopedia of Nuclear Magnetic Resonance"" JOHN WILEY & SONS, 6 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tetsuo Asakura: "Structure of Bombyx mori Silk Fibroin Studied by REDOR NMR Spectroscopy" Polymer Journal. 26. 1405-1408 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tetsuo Asakura: "Determination of the Structure of |1-^<13>C| Glycine-|-^<15>N| Alanine Double Labeled Bombyx mori Silk Fibroin Fibers Using Solid State^<13>NNMR" CHEMISTRY LETTERS. 2249-2252 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masashi Minami: "A Study on the Hydration of Bombyx mori Silk Fibroin by Nuclear Magnetic Resonance Spectroscopy" SEN-I GAKKAISHI. 50. 498-504 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Joo-Hong Yeo: "Structural analysis of highly oriented poly(p-phenylene terephthalamide) by ^<15>N solid-state nuclear magnetic resonance" Solid State Nuclear Magnetic Resonance. 3. 209-218 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Testuo Asakura: "Structure of Polyamide Fibers in Non-Crystalline Domain Studied by ^<15>N Solid State NMR" Polymer Jounal. 26. 229-233 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Joo-Hong Yeo: "Structural analysis of poly(m-xylene-α,α'-diyladipamide) and nylon 66 by ^<15>N solid state NMR" Macromol.Chem.Phys.195. 1423-1431 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 朝倉 哲郎: "合成高分子「構造解析」 "新高分子実験学5巻「高分子の構造(1)磁気共鳴」"" 共立出版, (印刷中) (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 出村 誠: "高分子「高分子材料の機器分析事例集」" 技術情報協会, 580 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tetsuo Askura: "NMR Characterization of Silk Proteins,ACS Symposium Series;Biology,Structure,Properites,Gentics." American Chemical Society Books Department, 370 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi