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Joint study on deltorphines in the mammalian brain

Research Project

Project/Area Number 06044107
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionShiga University of Medical Science

Principal Investigator

KIMURA Hiroshi  Shiga University of Medical Science, Molecular Neurobiology Research Center, Professor, 分子神経神経生物学研究センター, 教授 (40079736)

Co-Investigator(Kenkyū-buntansha) TINDARO Rend  ローマ大学, 医学部(イタリア共和国), 教授
MAEDA Toshihiro  Shiga University of Medical Science Department of Anatomy, Professor, 医学部, 教授 (50028388)
TOOYAMA Ikuo  Shiga University of Medical Science, Molecular Neurobiology Research Center, Ass, 分子生物学研究センター, 助教授 (20207533)
RENDA Tindaro  University of Rome Institute of Human Anatomy, Professor
RENDA Tindar  ローマ大学, 医学部(イタリア共和国), 教授
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordsdeltorphine / monoclonal / polyclonal / brain / antibody / dopaminergic / mRNA / opioid
Research Abstract

[D-Ala2] Deltorphin I (Tyr-D-Ala-Phe-Asp-Val-Val-Gly-NH2 : DADTI), an opioid peptide recently isolated from the skin of the south African frog Phyllomedusa bicolor, displays high affinity and selectivity for delta-opioid receptors. cDNAs encoding deltorphin precursors have been cloned from the skin of the same amphibian species. The precursors contain three DADTI sequences and only a single [D-Ala2] deltorphin II sequence. Because its L-isomer is pharmacologically inactive, D-alanine or at least D-amino acid in the second position appears essential to its opiate-like activity. In other words, the carboxy-terminal region of deltorphines seems crucial for addressing the molecules towards the delta-type receptor. More recently, Professor Tindaro Renda and Assistant Professor Ikuo Tooyama, both are the members of this joint project, have demonstrated that DADTI-binding sites by radioautography in brains of the rat and mouse. When injeced into the rat cerebral ventricle, DADTI induces sever … More al central effects including stimulation of locomotor activity, sniffing, rearing, grroming, as well as facillitation of social contacts and memory consolidation.
In addition to the findings described above, we have succeeded in the production of both polyclonal and monoclonal antibodies that specifically recognizes its carboxy-terminal tetrapeptide region. Immunohistochemical studies of mouse ad rat brains have detected DADTI-immunoreactive neuronal cell bodies and their processes in certain brain regions. These positive structures in the brain have been mapped in both adult and infant animals. We have also published a paper concerning DADTI-positive neurons in the substantia nigra, in relation with the coexistence of dopamine and calbindin-28K.The results were discussed with possible physiological function of DADTI in the brain. Finally, our study using molecular biology is progressing to identify the existence of mRNA encoding DADTI-like molecule in the mammalian brain, and the results will be published after the precise determination of the amino acid sequence. It is therefore concluded that the joint study supported by this grant has been sucessful, and a further study is promising on this novel peptide as an bioctive neuropeptide in mammals. Less

Report

(2 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Abe H: "Production of antiserum to [D-Ala^2] deltorphin I and its application to the mouse brain." Neuroreport. 3. 669-672 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tooyama I: "Immunohistochemical localization of [D-Ala^2] deltorphin I in adult rat brain." Proc Natl Acad Sci. 90. 9635-39 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abe H: "Immunohistochemical demonstration of [D-Ala^2] deltorphin I in amacrine cells of rat retina." Peptides.15. 49-54 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nonomura K: "Postnatal development of [D-Ala^2] deltorphin-I immunoreactive structures in the rat brain." J Comp Neurol. 349. 223-243 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui S: "Localization of [D-Ala^2] deltorphin I-like immunoreactivity in perinatal rat respiratory system." Histochem J. 26. 648-654 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujimiya M: "Demonstration of [D-Ala^2] deltorphin I-like immunoreactivity in mucosal cells of the rat gastrointestinal" Peptides. 15. 1095-1100 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui J: "J Histochem Cytochem" Transient expression on [D-Ala^2] deltorphin I-like immunoreactivity in prenatal rat small intestine., 1377-1381 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuhara O: "Acta Neuropathol" Pick's disease immnohistochemistry ; new aiterations and Alzheimer's disease comparisons., 322-330 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujimiya M: "Peptides" Effect of carbachol on the release of peptide YY from isolated vascularly and luminally perfused, 939-944 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakayasu H: "Ann NY Acad Sci" Cerecral GABA_A and GABA_B receptors., 516-527 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tooyama I: "Proceedings of the 2nd International malpighi symposium" Bioactive and polypeptides in the nigrostriatal neuronal pathway of the human brain.,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura H: "Proceedings of the 2nd International malpighi Symposium." Production of antibodies to [D-Ala^2] deltoephin I and its immunohistochemical application to rodent brain.,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abe H: "Production of antiserum to [D-Ala^2] deltorphin I and its immunohistochemical application to the mouse brain." Neuroreport. 3. 669-672 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tooyama I: "Immunohistochemical localization of [D-Ala^2] deltorphin I in adult rat brain." Proc Natl Acad Sci. 90. 9635-9639 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abe H: "Immunohistochemical demonstration of [D-Ala^2] deltorphin I in amacrine cells of rat retina." Peptides. 15. 49-54 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nonomura K: "Postnatal development of [D-Ala^2] deltorphin-I immunoreactive structures in the rat brain." J.Comp.Neurol.349. 223-243 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui S: "Localization of [D-Ala^2] deltorphin I-like immunoreactivity in perinatal rat respiratory system." Histochem.J.26. 648-654 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujimiya M: "Demonstration of [D-Ala^2] deltorphin I-like immunoreactivity in mucosal epithelial cells of the rat gastrointestinal tract." Peptides. 15. 1095-1100 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui J: "Transient expression on [D-Ala^2] dltorphin I-like immunoreactivity in prenatal rat small intestine." J.Histochem.cytochem.42. 1377-1381 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuhara O: "Pick's diseaseimmunohistochemistry : new aiterations and Alzheimer's disease comparisons." Acta Neuropathol.89. 322-330 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujimiya M: "Effect of carbachol on the release of peptide YY from isolated vascularly and luminally perfused rat ileum." Peptides. 16. 939-944 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakayasu H: "Cerebral GABA_A and GABA_B receptors." Ann.NY Acad.Sci.757. 516-527 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tooyama I: "Bioactive oligo and polypeptides in the nigrostriatal neuronal pathway of the human brain" Proceedings of the 2nd International malpighi symposium. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura H: "Production of antibodies to [D-Ala2] deltorphin I nad its immunohistochemical application to rodent brain." Proceedings of the 2nd International malpighi symposium. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abe H.: "Immunohistochemical demonstration of [D-Ala^2]deltorphin I in amacrine cells of rat retina." Peptides. 15. 49-54 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsuo A.: "Immunohistochemical locaization in the rat brain of an epitope corresponding to fibroblast growth factor receptor I." Neuroscience. 60. 49-66 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nonomura K: "Postnatal development of [D-Ala^2]deltorphin-I immunoreactive structures in the rat brain." J.Comp.Neurol.349. 223-243 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsui S.: "Localization of [D-Ala^2]deltorphin I-like immunoreactivity in perinatal rat respiratory system." Histochemical J.26. 648-654 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fijimiya M.: "Demonstration of [D-Ala^2]deltorphin I-like immunoreactivity in mucosal epithelial cells of the rat gastrointestinal tract." Peptides. 15. 1095-1100 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsui J.: "Transient expression of [D-Ala^2]deltorphin I-like immunoreactivity in prenatal rat small intestine." J.Histochem.Cytochem.42. 1377-1381 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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