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Structure and function of glycoprotein hormone receptors

Research Project

Project/Area Number 06044128
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KOSUGI Shinji  KYOTO UNIVERSITY,Assistant, 医学研究科, 助手 (50252432)

Co-Investigator(Kenkyū-buntansha) KOHN Leonald d.  NIDDK,Cell Regulation Section, Chief, Chief
SUGAWA Hideo  KYOTO UNIVERSITY,Lecturer, 医学研究科, 講師 (70162857)
AKAMIZU Takashi  KYOTO UNIVERSITY,Assistant, 医学研究科, 助手 (20231813)
MORI Toru  KYOTO UNIVERSITY,Professor, 医学研究科, 教授 (40026894)
KOHN Lenonar  NIDDK, Cell Regul
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1995: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1994: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsTSH receptor / LH receptor / FSH receptor / glycoprotein hormone receptor / signal transduction / constitutive activation / Familial Male Precocious Puberty / hyperfunctioning thyroid adenoma / cAMP / イノシトール燐酸 / 活性化型変異 / 分子生物学
Research Abstract

We investigated signal transduction mechanism of the TSH receptor by site-directed mutagenesis of the three cytoplasmic loops and C-terminal tail and following observations were obtained. For Gs interaction, the middle portion of the second cytoplasmic loop is most important. For Gq interaction, widely distributed regions such as entire region of the first cytoplasmic loop, the middle portion of the second cytoplasmic loop, the N-terminal and C-terminal portions of the third cytoplasmic loop and N-terminal one third of the C-terminal tail are crucial. Substitution mutants at residues 617-620 in the third cytoplasmic loop showed constitutive activation of the cAMP and inositol phosphate levels. This is the first example of constitutively activated glycoprotein hormone receptor mutations. After that, TSH receptor activating mutations in hyperfunctioning thyroid adenomas were reported. We also showed that TSH receptor mutations Asp^<633>*Glu/Tyr, Thr^<622>*Ile, and Phe^<631>*Cys found in hyperfunctioning thyroid adenomas were constitutively activated by expression experiments and proved that those were direct cause of the disease. All activating glycoprotein hormone receptor mutation so far reported were located in the transmembrane region. We showed that a deletion mutant in the extracellular domain was also constitutively activated. Further, exoplasmic loop was shown to be related signal transduction also. The letter two observations suggest that interaction between the extracellular region and the transmembrane region is important event for signal transdution.
As for LH receptor, we also found new activation mutations in familial and sporadic male precocious puberty and showed that those were the direct cause of the disease by expression experiments. We also investigated the mechanism of activation of the LH receptor by in vitro mutagenesis and found that the importance of the side chain of the Asp^<578> for maintaining the inactive state of the receptor.

Report

(2 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (85 results)

All Other

All Publications (85 results)

  • [Publications] Kosugi S.et al.: "The middle portion in the second cytoplasmic loop of the thyrotropin receptor plays a crucial role in adenylate cyclase activation." Molecular Endocrinology. 8. 498-509 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.,Mori T.: "The first cytoplasmic loop of the thyrotropin receptor is important for phosphoinositide signaling but not for agonist-induced adenylate cyclaseactivation." FEBS Letters. 341. 162-166 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.,Mori T.: "The intracellular region adjacent toplasma membrane (residues 684-692) of the thyrotropin receptor is important for phosphoinositide signaling but not for agonist-induced adenylate cyclase activation." Biochem Biophys Res Commun. 199. 1497-1503 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.,Mori T.: "The amoni-terminal half of the cytoplasmic tail of the thyrotropin receptor is essential for full activities of receptorfunction." Biochem Biophys Res Commun. 200. 401-407 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.,et al.: "Possible difference in glycosylation of the thyrotropin receptor among species." Biochem Biophys Res Commun. 200. 1207-1213 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.,Mori T.: "The third exoplasmic loop of the thyrotropin receptor is partially involved in signal transduction." FEBS Letters. 349. 89-92 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.,Shenker A.,Mori T.: "Constitutive activation of cyclic AMP signal but not phospatidylinostol signaling caused by four mutaions in the 6th transmembrane helix of the human thyrotropin receptor." FEBS Letters. 356. 291-294 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.et al.: "Characterization of heterogeneous mutations causing constitutive activation of luteinizing hormone receptor in familial male precociouspuberty." Human Molecular Genetics,. 4. 183-188 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Akamizu T.et al.: "Further studies of amino acids (268-304) in TSH-LH/CG chimeric receptors : cysteine-301 is important in TSH binding and receptor tertiary structure." Thyroid. 4. 43-48 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mori T.et al.: "Recent progress in TSH receptor studies with a new concept of “autoimmune TSH receptor disease".Endocrine Journal 41 1994 1-11" Endocrine Journal. 41. 1-11 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ueda Y.,Sugawa H.et al.: "Thyroid-stimulating antibodies in sera from patients with Graves' disease are heterogeneous in epitope recognition." Eur J Endocrinol. 132. 62-68 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okuda J.,Akamizu T.et al.: "Preparation and characterization of monoclonal antithyrotropin receptor antibodies obtained from peripheral lymphocytes of hypothyroid patients with primary mxedema." J Clin Endocrinol Metab. 79. 1600-1604 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司、森徹: "TSH受容体研究の最近の動向:構造的特徴とリガンド・自己抗体の結合部位、糖鎖の意義および情報伝達との関係" 日本内分泌学会雑誌. 70. 65-68 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司、森徹: "TSH受容体の情報伝達" 日本臨床. 52. 984-989 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司: "TSH受容体のTSH,自己抗体の結合部位" ホルモンと臨床. 42. 673-682 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 赤水尚史 他: "抗TSHレセプター抗体の結合部位;部位特異的変異法による検討" 日本臨床. 52. 1024-1030 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司: "下垂体性糖蛋白ホルモン受容体の機能的解析と臨床応用" 興和生命科学振興財団研究報告集. 6. 160-167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司: "下垂体性糖蛋白ホルモン(TSH,LH,FSH)受容体の構造と機能の解析と甲状腺、性腺の難治性疾患治療への臨床応用" 持田記念財団研究成果報告集. 11. 168-175 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司: "プランマー病のTSH-R遺伝子変異" Modern Physician. 15. 777-780 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司、森徹: "家族性男子思春期早発症(FMPP)の病因-黄体化ホルモン受容体(LHR)の活性化型変異" 医学のあゆみ. 169. 864-865 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司、森 徹: "黄体化ホルモン受容体の活性化型変異" 医学のあゆみ. 173. 714-715 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.,Mori T.: "TSH receptor and LH receptor,1995" Endocrine J. 42. 587-606 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.,Mori T.: "TSH receptor and LH receptor,1996" (in preparation).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.et al: "Severe testotoxicosis phenotype with a strongly activating mutation of the lutropin receptor gene" (in submission).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司、森 徹: "家族性思春期早発症" Annual Review 内分泌代謝. (準備中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司、森 徹: "甲状腺自己抗原と抗体-TSH受容体の構造と機能" 医学のあゆみ. (準備中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugawa H,Akamizu T,Kosugi S.et al.: "Presence of heterogeneous thyroid-stimulating antibodies in sera from individual Graves' patients as shown by synthesized thyrotropin receptor peptide application : evidence showing two independent epitopes and a possible recognition of two epitopic regions by one antibody molecule." Eur.J.Endocrinol.133. 283-293 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Zhang ML,Sugawa H,Kosugi S.Mori T: "Constitutive activation of the thyrotropin receptor by deletion of a portion of the extracellular domain" Biochem.Biophys.Res.Commun.211. 205-210 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Li H,Akamizu T,Okuda J,Mori T Sugawa H,Matsuda F,Tsubata T,: "Isolation of Epstein-Barr-virus-transformed lymphocytes producing IgG class monoclonal antibodies using a magnetic cell separator (MACS) : preparation of thyroid-stimulating IgG antibodies from patients with Graves' disease" Biochem.Biophys.Res Commun.207. 985-993 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakamura N,Akamz C,Akamizu T,Sugawa H,Mitsuda N,Honjo T,Mori T,Yamaji K,Matsuda F,: "Heterogeneity and diversity of IgM and IgG lupus anticoagulants in an individual with systemic lupus erythematosus" Biochem.Biophys.Res Commun.203. 1789-1794 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shin EK,Akamizu T,Matsuda F,Sugawa H,Fujimura J,Mori T,Honjo T: "Variable regions of Ig heavy chain genes encoding antithyrotropin receptor antibodies of patients with Graves'disease" J.Immunol. 152. 1485-1492 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Torizuka T,Tamaki N,Kasagi K,Misaki T,Tadamura E,Magata Y,Yonekura Y,Mori T,Kawamoto M,Konishi J: "Myocardial oxidative metabolism in hyperthyroid patients assessed by PET with carbon-11-acetate" J.Nucl.Med. 36. 1981-1986 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kasagi K,Takeuchi R,Miyamoto S,Misaki T,Shimazu A,Mori T,Konishi J,Inoue D,: "Metastatic thyroid cancer presenting as thyrotoxicosis : report of three cases" Clin.Endocrinol.Oxf.40. 429-434 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 森 徹: "甲状腺疾患の初期検査" 臨床病理. 43. 586-592 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 渋谷雄平、井上大輔、須川秀夫、森 徹: "同時性に甲状腺乳頭癌・胃癌・直腸癌を合併した一例" 日本内分泌学会誌. 70. 951-956 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kohn L.D.,Kosugi S.et al.: "Molecular Endoclinology : Basic Concepts and Clinical Correlations." ed.Weintraub B.D.,Raven Press.New York,Cloning and regulation of glycoprotein hormone rereptor genes., 21 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司、森徹: "臨床DNA診断法:黄体化ホルモン(LH)受容体異常症" 古庄敏行、井村裕夫編(金原出版), 3 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.et al.: "The middle portion in the second cytoplasmic loop of the thyrotropin receptor plays a crucial role in adenylate cyclase activation." Molecular Endocrinology. 8. 498-509 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Mori T.: "The first cytoplasmic loop of the thyrotropin receptor is important for phosphoinositide signaling but not for agonist-induced adenylate cyclaseactivation" FEBS Letters. 341. 162-166 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Mori T.: "The intracellular region adjacent toplasma membrane (residues 684-692) of the thyrotropin receptor is important for phosphoinositide signaling but not for agonist-induced adenylate cyclase activation." Biochem Biophys Res Commun. 199. 1497-1503 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Mori T.: "The amoni-terminal half of the cytoplasmic tail of the thyrotropin rceptor is essential for full activities of receptor function." Biochem Biophys Res Commun. 200. 401-407 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., et al.: "Possible difference in glycosylation of the thyrotropin receptor among species." Biochem Biopys Res Commun. 200. 1207-1213 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Mori T.: "The third exoplasmic loopd of the thyrotropin receptor is partially involved in signal transduction." FEBS Letters. 349. 89-92 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Shenker A., Mori T.: "Constitutive activation of cyclic AMP signal but not phospatidylinostol signagling caused by four mutationsin the 6th transmembrane helix of the human thyrotropin receptor." FEBS Letters. 356. 291-294 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.et al.: "Characterization of heterogeneous mutations causing constitutive activation of luteinizing hormone receptor in familial male precocious puberty." Human Molecular Genetics. 4. 183-188 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Akamizu T.et al.: "Further studies of amino acids (268-304) in TSH-LH/CG chimeric receptors : cysteine-301 is important in TSH binding and receptor tertiary structure." Thyroid. 4. 43-48 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mori T.et al.: "Recent progress in TSH receptor studies with a new concept of "autoimmune TSH receptor disease"." Endocrine Journal. 41. 1-11 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ueda Y., Sugawa H.et al.: "Thyroid-stimulating antibodies in sera from patients with Graves'disease are heterogeneous in epitope recognition." Eur J Endocrinol. 132. 62-68 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okuda J., Akamizu T.et al.: "Preparation and characterization of monoclonal antithyrotropin receptor antibodies obtained from peripheral lymphocytes of hypothyroid patients with primary mxedema." J Clin Endocrinol Metab. 79. 1600-1604 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Mori T: "Recent progress in TSH receptor research : relationship of its structual characteristics to ligand binding, glycosyslation and signal transduction.(Japanese)" Nippon Naibunpi Gakkai Zasshi. 70. 65-68 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Mori T: "Signal transduction of the thyrotropin receptor.(Japanese)" Nippon Rinsho. 52. 984-989 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S: "Binding site of TSH and autoantibodies on the TSH receptor.(Japanese)" Clinical Endocrinology(Tokyo). 42. 673-682 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Akamizu T.et al.: "Binding site of anti-TSH receptor antibody : site-directed mutagenesis approach.(Japanese)" Nippon Rinsho. 52. 1024-1030 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S: "Function analysis and clinical implications of pituitary glycoprotein hormone receptors.(Japanese)" Annual report of Kowa Lifescience Foundation. 6. 160-167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S: "Analysis of structure and function of pituitary glycoprotein hormone (TSH,LH,FSH) receptors and clinical implications.(Japanese)" Annual report of Mochida Foundation. 11. 168-175 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S: "TSH-R gene abnormalities of Plummer's disease.(Japanese)" Modern Physician. 15. 777-780 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S,Mori T: "Couse of familical male precocious puberty : activating mutations of LHR.(Japanese)" Igaku no Ayumi. 169. 864-865 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S,Mori T: "Activating mutations of lutropin receptor.(Japanese)" Igaku no Ayumi. 173. 714-715 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Mori T.: "TSH receptor and LH receptor, 1995" Endocrine J. 42. 587-606 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S., Mori T.: "TSH receptor and LH receptor, 1996" in preparation.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S.et al: "Severe testotoxicosis phenotype with a strongly activating mutation of the lutropin receptor gene in submission"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugawa H,Akamizu T,Kosugi S.et al.: "Presence of heterogeneous thyroid-stimulating antibodies in sera from individual Graves' patients as shown by synthesized thyrotropin receptor peptide application : epitopic regions by one antibody molecule." Eur.J.Endocrinol.133. 283-293 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Zhang ML,Sugawa H,Kosugi S.Mori T: "Constitutive activation of the thyrotropin receptor by deletion of a portion of the extracellular domain" Biochem.Biophys.Res.Commun.211. 205-210 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Li H,Akamiu T,Okuda J,Mori T,Sugtawa H,Matsuda F,Tsubata T: "Isolation of Epstein-Barr-Virus-transformed lymphocytes producing IgG class monoclonal antibodies using a magnetic cell separator (MACS) : preparation of thyroid stimulating IgG antibodies from patients with Graves'disease" Biochem.Biophys.Res.Commun.207. 985-993 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakamura N,Akamz C,Akamizu T,Sugawa H,Matsuda F,Mitsuda N,Honjo T,Mori T,Yamaji K: "Heterogeneity and diversity of IgM and IgG lupus anticoagulants in an individual with systemic lupus erythematosus" Biochem.Biophys.Res.Commun.203. 1789-1794 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shin EK,Akamizu T,Matsuda F,Sugawa H,Fujimura J,Mori T,Honjo T: "Variable regions of Ig Heavy chain genes encoding antithyrotropin receptor antibodies of patients with Graves'disease" J.Immunol. 152. 1485-1492 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Torizuka T,Tamaki N,Kasagi K,Misaki T,Kawamoto M,Tadamura E,Magata Y,Yonekura Y,Mori T,Konishi J: "Myocardial oxidative metabolism in hyperthyroid patients assessed by PET with carbon-11-acetate" J.Nucl.Med. 36. 1981-1986 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kasagi K,Takeuchi R,Miyamoto S,Misaki T,Inoue D,shimazu A,Mori T,Konishi J: "Metastatic thyroid cencer presenting as thyrotoxicosis : report of three cases" Clin.Endocrinol.Oxf.40. 429-434 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shibutani Y,Inoue D,Sugawa H,Mori T: "A case report of primary triple cancers in the thyroid, stomach and rectum with evidence of variable oncoprotein expressions" Nippon-Naibunpi-Gakkai-Zasshi. 70. 951-956 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mori T: "Initial tests for examining patients with thyroid disorders" Rinsho-Byori. 43. 586-592 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S,Mori T: "Familial male precocious puberty.(Japanese)" Annual Review : Endocrinology and Metabolism. (In Preparation). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S,Mori T: "Thyroidal autoantigens and autoantibodies : sturucture and function of TSH receptor.(Japanese)" Igaku no Ayumi. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kohn L.D., Kosugi S.et al.: ed.Weintraub B.D., Raven Press, New York, Cloning and regulation of glycoprotein hormone rereptor genes.Molecular Endoclinology : Basic Concepts and Clinical Correlations., 133-153 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kosugi S: Clinical DNA Diagnosis. LH receptor abnormalities.(Japanese), 489-491 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小杉眞司: "プランマー病のTSH-R遺伝子変異" Modern Physician. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小杉眞司、森徹: "家族性男子思春期早発症(FMPP)の病因-黄体化ホルモン受容体(LHR)の活性化型変異" 医学のあゆみ. 169. 864-865 (1994)

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      1994 Annual Research Report
  • [Publications] 小杉眞司: "LH receptor異常" 臨床DNA診断法. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小杉眞司、森徹: "黄体化ホルモン受容体の活性化型変異" 医学のあゆみ. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kosugi S.et al.: "The middle portion in the second cytoplasmic loop of the thyrotropin receptor plays a crucial role in adenylate cyclase activation." Molecular Endocrinology. 8. 498-509 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kosugi S.,Mori T.: "The first cytoplasmic loop of the thyrotropin receptor is important for phosphoinositide signaling but not for agonist-induced adenylate cyclaseactivation." FEBS Lettters. 341. 162-166 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kosugi S.,Mori T.: "The intracellular region adjacent toplasma membrane (residues 684-692) of the thyrotropin receptor is important for phosphoinositide signaling but not for agonist-induced adenylate cyclase activation." Biochem Biophys Res Commun. 199. 1497-1503 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kosugi S.,Mori T.: "The amoni-terminal half of the cytoplasmic tail of the thyrotropin receptor is essential for full activities of receptorfunction." Biochem Biophys Res Commun. 200. 401-407 (1994)

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      1994 Annual Research Report
  • [Publications] Kosugi S.,et al.: "Pssible difference in glycosylation of the thyrotropin receptor among species." Biochem Biopys Res Commun. 200. 1207-1213 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kosugi S.,Mori T.: "The third exoplasmic loop of the thyrotropin receptor is partially involved in signal transduction." FEBS Letters. 349. 89-92 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kohn L.D.,Kosugi S.et al.: "Molecular Endoclinology:Basic Concepts and Clinical" ed.Weintraub B.D.,Raven Press,New York,Cloning and regulation of glycoprotein hormone rereptor genes., 21 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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