| Project/Area Number |
06044152
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Okayama University, Medical School |
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Principal Investigator |
OGUMA Keiji Okayama University, Medical School, Professor, 医学部, 教授 (00002262)
小熊 恵二 岡山大学, 医学部, 教授
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| Co-Investigator(Kenkyū-buntansha) |
JANET Holden ジェームスクック大学, 獣医学部, 研究員
ROBERT Hirst ジェームスクック大学, 獣医学部, 助教授
YOKOTA Kenji Okayama University, Medical School, Research associate, 医学部, 助手 (00243460)
INOUE Kaoru Okayama University, Medical School, Research associate, 医学部, 助手 (40260103)
HIRST Robert James Cook University, Department of Veterinary Medicine, Associate professor
HOLDEN Janet James Cook University, Department of Veterinary Medicine, Researcher
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| Project Period (FY) |
1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Botulinum toxin / Botulism / Vaccine / Serum therapy / ELISA / Toxin gene |
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| Research Abstract |
The type C and D botulinum strains were isolated from the ground of a cattle farm (a pasture) in Australia. The type C toxin was purified from one type C culture, and its antigenicity was analyzed by monoclonal antibodies. Based on the results of antigenicity and toxin-neutralization tests, it was concluded that the C-terminal of the toxin can be used as effective vaccine.
The peptide (15 amino acid residues) which is conserved in the light chain components of any different types of botulinum toxins was synthesized, and the monoclonal antibodies against it were prepared in rats. Though two monoclonal antibodies reacting with any types of toxins were obtained, they did not neutralize the toxigenicity, indicating that this peptide can not be used as vaccine, but the monoclonal antibodies thus obtained can be used for diagnosis of botulism caused by any types of toxins.
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