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Biological Function of Type VIII Collagen

Research Project

Project/Area Number 06044154
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionOkayama University Medical School

Principal Investigator

NINOMIYA Yoshifumi  Okayama University Medical School, Professor, 医学部, 教授 (70126241)

Co-Investigator(Kenkyū-buntansha) MATT Warman  ケースウェスタンリザーブ大学, 医学部, 助教授
O;ENA Jacenk  ハーバード大学, 医学部, 研究員
SUNEEL Apte  ハーバード大学, 医学部, 研究員
OOHASHI Toshitaka  Okayama University Medical School, Assistant Professor, 医学部, 助手 (50194262)
MURAGAKI Yasuteru  Wakayama Medical College, Associate Professor, 病理学, 講師 (40190904)
BJORN Olsen  ハーバード大学, 医学部, 教授
APTE Suneel  Harvard Medical School, Research Associate
OLSEN Bjorn  Harvard Medical School, Professor
WARMAN Matt  Case Western Reserve University, Assistant Professor
JACENKO Olena  Harvard Medical School, Research Associate
Project Period (FY) 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1994: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsType VIII Collagen / Smooth Muscle Cells / Keratinocytes / Transgenic Mice
Research Abstract

In order to know which cells or organs produce type VIII collagen, we extracted RNA from various cells and tissues and performed Northern blot analysis using specific cDNAs. Cultured human aortic endothelial cells were found to keep synthesizing alpha1 (VIII) and alpha2 (VIII) chains until tenth passage. These cells synthesize collagen type I,III,and V at the same time. In separate experiment rat aortic smooth muscle cells were tested whether they produce collagens. What we detected was that the cells synthesized 75 kDa bacterial collagenase sensitive materials. Of interest was that production of this materials was enhanced 20 to 30 times by addition of heparin into the culture medium. Currently we are testing whether this bacterial collagenase sensitive material is the alpha-chain of type VIII by Northern blotting analysis. We also demonstrated that mouse keratinocytes produce collagen VIII at the mRNA level using Northern-blotting and in situ hybridization.
Several lines of transgenic mice did show strange expression pattern of type VIII collagen gene during development. It seemed that its pattern was strict and developmentally regulated. However, we did not know whether the pattern was artifact or real, since we did not predict the expression pattern ; transient in certain tissues. Direct in situ hybridization was performed using the specific probes for mouse collagen type VIII at the developmental stage and proved that the expression pattern was real.

Report

(1 results)
  • 1994 Final Research Report Summary
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Oohashi T.,Sugimoto M.,Mattei M.-G.,and Ninomiya Y.: "Identification of a new collagen IV chain,α6(IV),by cDNA isolation and assignment of the gene to chromosome Xq22,which is the same locus for COL4A5." J Biol Chem. 269. 7520-7526 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugimoto M.,Oohashi T.,and Ninomiya Y.: "The two genes,COL4A5 and COL4A6,coding for the two new basement membrane collagen chains,α5(IV) and α6(IV),are located head-to-head in close proximity on chromosome Xq22 and COL4A6 is transcribed from two alternate promoters." Proc.Natl.Acad.Sci.U.S.A.91. 11679-11683 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iyama K.-I.,Kitaoka M.,Monda M.,Ninomiya Y.,and Hayashi M.: "Co-expression of collagen types II and XmRNAs in newly formed hypertrophic chondrocytes of the embryonic chick vertebral body demonstrated by double fluorescence in situ hybridization." Histochem.J.26. 844-849 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Inoguchi K.,Yoshioka H.,Khaleduzzaman M.,and Ninomiya Y.: "The mRNA for the α(XIX)collagen chain,a new member of FACITs,contain a long unusual 3′ untranslated region and displays many unique splicing variants." J.Biochem.(Tokyo). 117. 137-146 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshioka H.,Greenwel P.,Inoguchi K.,Truter S.,Inagaki Y.,Ninimiya Y.,and Ranirez F.: "Structural and functional analysis of the promoter of the human α(XI) collagen gene." J.Biol.Chim.270. 418-424 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Li Y.,Lacerda D.A.,Warman M.L.,Beier D.R.,Yoshioka Y.,Ninomiya Y.,Oxford J.T.,Morris N.P.,Andrikopoulos K.,Ramirez F.,Wardell B.B.,Lifferth G.D.,Teuscher C.,Woodward S.R.,Taylor B.A.,Seegmiller R.E.,and Olsen B.R.: "A fibrillar collagen gene,Col11al,is essential for skeletal morphogenesis." Cell. 80. 423-430 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 二宮善文: "IV型コラーゲンの遺伝子構造とその発現調節" 医学と薬学32巻4号,日本DPCコ-ポレーション, 701-705 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 二宮善文,吉岡秀克,阿部信寛: "軟骨コラーゲン" THE BONE Vol.8 No.4(株)メディカルレビュー社, 39-49 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 吉岡秀克,大橋俊孝,二宮善文: "腎糸球体のコラーゲン" 腎と透析 第37巻第6号 東京医学社, 1075-1083 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Oohashi T., Sugimoto M., Mattei M.-G., and Ninomiya Y.: "Identification of a new collagen IV chain, alpha6 (IV), by cDNA isolation and assignment of the gene to chromosome Xq22, which is the same locus for COL4A5." J Biol Chem. 269. 7520-7526 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugimoto M., Oohashi T., and Ninomiya Y.: "The two genes, COL4A5 and COL4A6, coding for the two new basement membrane collagen chains, alpha5 (IV) and alpha6 (IV), are located head-to-head in close proximity on chromosome Xq22 and COL4A6 is transcribed from two alternate promoters." Proc.Natl.Acad.Sci.U.S.A.91. 11679-11683 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iyama K.-I., Kitaoka M., Monda M., Ninomiya Y., and Hayashi M.: "Co-expression of collagen types II and X mRNAs in newly formed hypertrophic chondrocytes of the embryonic chick vertebral body demonstrated by double fluorescence in situ hybridization." Histochem.J.26. 844-849 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Inoguchi K., Yoshioka H., Khaleduzzaman M., and Ninomiya Y.: "The mRNA for the alpha1 (XIX) collagen chain, a new member of FACTTs, contain a long unusual 3'untranslated region and displays many unique splicing variants." J.Biochem. (Tokyo). 117. 137-146 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshioka H., Greenwel P., Inoguchi K., Truter S., Inagaki Y., Ninomiya Y., and Ramirez: "Structural and functional analysis of the promoter of the human alpha1 (XI) collagen gene." J.Biol.Chem.270. 418-424 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Li Y., Lacerda D.A., Warman M.L., Beier D.R., Yoshioka Y., Ninomiya Y., Oxford J.T., Morris N.P., Andrikopoulos K., Ramirez F., Wardell B.B., Lifferth G.D., Teuscher C., Woodward S.R., Taylor B.A., Seegmiller R.E., and Olsen B.R.: "A fibrillar collagen gene, Col11a1, is essential for skeletal morphogenesis." Cell. 80. 423-430 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshifumi Ninomiya: Medicine and Pharmacology. Structure and regulation of collagen type IV genes, 701-705 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshifumi Ninomiya, Hidekatsu Yoshioka, and Nobuhiro Abe: Medical Review Corporation. Cartilage collagens, 39-49 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hidekatsu Yoshioka, Toshitaka Oohashi, and Yoshifumi Ninomiya: Tokyo Medicals. Glomerular collagens in kidney, 1075-1083 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary

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Published: 1994-04-01   Modified: 2016-04-21  

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