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Breeding of nitrogen-fixation becteria in Southeast Asia

Research Project

Project/Area Number 06044159
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionOsaka University (1995)
Hiroshima University (1994)

Principal Investigator

MUROOKA Yoshikatsu  Professor, Osaka University, Faculty of Engineering, 工学部, 教授 (60029882)

Co-Investigator(Kenkyū-buntansha) OZAWA Takashi  Assistant Professor, Osaka Prefecture University, Faculty of Agriculture, 農学部, 助手 (20152481)
SEKI Tatsuji  Professor, Osaka University, International Center for Biotechnology, 生物工学国際交流センター, 教授 (50029245)
UOZUMI Takeshi  Professor, University of Tokyo, Faculty of Agriculture, 農学部, 教授 (40011978)
HIGASHI Shiro  Professor, Kagoshima University, Faculty of Science, 理学部, 教授 (60041216)
KOMAGATA Kazuo  Professor, Tokyo Agricultural University, Faculty of Agriculture, 農学部, 教授 (70013331)
NANYAKORN Bo  タイ国スラナリー工科大学, 生物工学部, 教授
BAYANI M Esp  フィリッピン大学, 生物工学研究所, 助教授
MADE Sri Pra  インドネシア国立科学研究所, 生物工学センター, 所長
BOONKERD Nantakorn  Professor, Suranaree University of Technology
PRAMA Made sri  Director, Indonesian National Institutes of Science, Center for Biotechnology
ESPIRITU Bayani m.  Associate Professor, University of Philippines, National Institute of Biotechnol
NANTAKORN Bo  タイ国スラナリー工科大学, 生物工学部, 教授
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥26,200,000 (Direct Cost: ¥26,200,000)
Fiscal Year 1995: ¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1994: ¥13,600,000 (Direct Cost: ¥13,600,000)
KeywordsNitrogen-fixing bacteria / Southeast Asia / Acidic soil / Nobulation / Genetic engineering / 窒素固定 / 遺伝子工学
Research Abstract

It has been many demands of collaborated works for nitrogenfixing bacteria from Asian researchers, since the bilogical nitrogen fixation is quite useful in poor soil, and there are common problems of this research in East Asian countries. Thus, we set up the joint research project that isolation, identification, breeding, and application of nitrogen-fixing bacteria in tropical area.
In particular, we focused on the isolation of acid tolerant Rhizobium strains from acidic soil in Thailand, Indonesia and Philippines. These newly isolated Rhizobia strains are identified by chemotaxonomical and genetical methods. Next, we selected useful strains which are acid tolerant and have high activity of nitrogen fixation. These strains are tried to further breedc to more powerful strain. These isolated and breed strains have been evaluated to be effective in the field of Thailand, Indonesia and Philippines.
Along with this project, in 1994 fiscal year, 3 Japanese researcheres visited Thailand, Indonesia and Philippines to do collaborated works for 7 to 14 days. Seven researchers from Thailand, Indonesia and Philippines were invited to Japanese research groups as collaborated works for 10 to 60 days in 1994. International Seminars of this poroject was held in Kohn Kaen, Thailand, October 13 to 15, 1994, and 14 collaborated researchers gave lectures and about 50 persons were attended.
In 1995 fiscal year, one senior researcher from Indonesia (10 days) and 3 junior researchers from Indonesia, Thailand and Philippines (50 days) were invited to Japanese University and worked with nitrogen-fixing bacteria isolated in their countries. International Seminar of this project was held in Yogjakarta and Denpasar, Indonesia and 17 collaborated researchers reported their results under this project and totally about 60 persons were attended the seminar and discussed the problems.

Report

(2 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Y.Xu,A.Yokota,K.Sanada,M.Hisamatsu,M.Araki,H.-J.Cho,T.Morinaga and Y.Murooka: "Enterobacter cloacae A105,isolated from the surface of root nodules of Astragalus sinicus cv. Japan,stimulates nodulation by Rhizobium huakuii bv. renge" Journal of Fermentation and Bioengineering. 77. 630-635 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Inoue,T.Shigematsu,M.Hidaka,H.Masaki and T.Uozumi: "Functions of DRAT and DRAG and the transcriptional regulation of dra TG operon in Azospirillum lipoferum" Abstract of 4th Annual Meeting for plant-Microbe Interacfions. 4. 82-84 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Akao,T.Nakayama,S.H.Kim,P.B.Francisco,S.Higashi: "Rhizobium host-range extension by introduction of Rhizobium trifolii nod gene" Soil Science and Plant Nutrition. 40. 703-708 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Higashi,T.Yoneyama and Sa.Higashi: "Forest-restoration sffects at Alnus hirsuta (Spach) Rupr. plantations on two small islands in northern Japan" Report of Faculty of Science,Kagoshima University. 27(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.J.Cho,Y.Xu and Y.Murooka: "Formation of adventitious shoots and plant regeneration by culture of cotyledon segment in Astragalus sinicus (Chinese Milk Vetch)" Plant Tissue Culture Letter. 12. 87-90 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.-J.Cho,H.Morikawa and Y.Murooka: "Expression pattern of bacterial polycistronic gene in tabaco cells" Journal of Fermentation and Bioengineering. 80. 276-279 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Xu and Y.Murooka: "A large plasmid isolated from Rhizobium huakuii bv. renge that include genes for both nodulation of Astragalus sinicus cv. Japan and nitrogen fixation" Journal of Fermentation and Bioengineering. 80. 276-279 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Uchiumi,S.Higashi and M.Abe: "Nodule formation by clover-Rhizobium carring chromosomal nod genes" Journal of Genaral and Applied Microbiology. 41. 9-20 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Ozawa,K.Kato and T.Matsunami: "Concomitant in hibition of nitrogen fixation and N-phenyl-1-naphylamine uptake activity of bacteroids of a Bradyrhizobium japonicum strain by nitrate" Soil Science and Plant Nutrition. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Teaumroong,Y.Murooka and N.Boonkerd: "Acid tolerance and antibiotic resistance of some strians of Bradyrhizobium applied in Thailand" Suranaree journal of Science and Technology. 2. 75-80 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Inoue,T.Shigematsu,M.Hidaka,H.Masaki and T.Uozumi: "Cloning,sequencing and transcriptional requlation of the draT and draG genes of Az-ospirillum lipoferum FS" Gene. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Abe,S.Higashi and T.Uchium: "Identification of intection signals between Rhizobium and its host Leguminoseae" Plant Cell Physiol.35. s63 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Uchiumi,M.Abe and S.Higashi: "Efficient expression of integrated chromosomal nod genes in Rhizobium leguminosarum bv. trifolii" Plant Cell Physiol.36. s121 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Uozumi(分担執筆)Eds.Y.Murooka and T.Imanaka: "Recombinant Microbes for Industrial and Agricultural Applications" Marcel Dekker,Inc.New York, 9 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Murooka and T.Imanaka: "Recombinant Microbes for Industrial and Agricultural Applications" Marcel Dekker,Inc.New York, 877 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 魚住武司: "微生物機能の多様性" 学会出版センター, 7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Abe,H.Yamashita,A.Higashi and T.Uchiumi(分担執筆)A.Tikhonovich et al.: "Nitrogen Fixation : Fundamentals and Applications" Kluwer Academic Publishers, 1 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Xu, A.Yokota, K.Sanada, M.Hisamatsu, M.Araki, H.-J.Cho, T.Morinaga and Y.Murooka: "Enterobacter cloacae A105, isolated from the surface of root nodules of Astragalus sinicus cv. Japan, stimulates nodulation by Rhizobium huakuii bv. renge" Journal of Fermentation and Bioengineering. 77. 630-635 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Inoue, T.Shigematsu, M.Hidaka, H.Masaki and T.Uozumi: "Functions of DRAT and DRAG and the transcriptional regulation of dra TG operon in Azospirillum lipoferum" Abstract of 4th Annual Meeting for plant-Microbe Interactions. 4. 82-84 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Akao, Y.Nakayama, S.H.Kim, P.B.Francisco, S.Higashi: "Rhizobium host-range extension by introduction of Rhizobium trifolii nod gene" Soil Science and Plant Nutrition. 40. 703-708 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Higashi, T, Yoneyama and Sa. Higashi: "Forest-restoration effects at Alnus hirsuta (Spach) Rupr. plantations on two small islands in northern Japan" Report of Facullty of Science, Kagoshima University. 27 (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.J.Cho, Y.Xu and Y.Murooka: "Formation of adventitious shoots and plant regeneration by culture of cotyledon segment in Astragulus sinicus (Chinese Milk Vetch)" Plant Tissue Culture Letter. 12. 87-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.-J.Cho, H.Morikawa and Y.Murooka: "Expression pattern of bacterial polycistronic gene in tabaco cells" Journal of Fermentation and Bioengineering. 80. 276-279 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Xu and Y.Murooka: "A large plasmid isolated from Rhizobium huakuii bv renge that includes genes for both nodulation of Astragalus sinicus cv. Japan and nitrogen fixation" Journal of Fermentation and Bioengineering. 80. 276-279 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Uchiumi, S.Higashi and M.Abe: "Nodule formation by clover-Rhizobium carrying chromosomal nod genes" Journal of General and Applied Microbiology. 41. 9-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Ozawa, K.Kato and T.Matsunami: "Concomitant inhibition of nitrogen fixation and N-phenyl-1-naphtylamine uptake activity of bacteroids of a Bradyrhizobium japonicum strain by nitrate" Soil Science and Plant Nutrition. (submitted). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Teaumroong, Y.Murooka and N.Boonkerd: "Acid tolerance and antibiotic resistance of some strains of Bradyrhizobiumapplied in Thailand" Suranaree journal of Science and Technology. 2. 75-80 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Inoue, T.Shigematsu, M.Hidaka, H.Masaki and T.Uozumi: "Cloning, sequencing and transcriptional regulation of the dra T and dra G genes of Azospirillum lipoferum FS" Gene. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Abe, S.Higashi and T.Uchium: "Identification of intection signals between Rhizobium and its host Leguminoseae" Plant Cell Physiol.35. s63 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Uchiumi, M.Abe and S.Higashi: "Efficient expression of integrated chromosomal nod genes in Rhizobium leguminosarum bv. trifolii" Plant Cell Physiol.36. s121 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Uozumi Eds. Y.Murooka and T.Imanaka: Marcel Dekker, Inc. New York. Recombinant Microbes for Industial and Agricultural Applications, 9 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Murooka and T.Imanaka: Marcel Dekker, Inc. New York. Recombinant Microbes for Industrial and Agricultural Applications, 877 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Uozumi: Gakkai syuppan center. Biodiversity of Microbial Functions, 7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Abe, H.Yamashita, A.Higashi and T.Uchiumi Eds. A.Tikhonovich et al.: Kluwer Academic Publishers. Nitrogen Fixation : Fundamentals and Applications, 1 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yong Xu: "Enterobacter cloacae A105,isolated from the surface of root nodules of Astragalus cinicus cv.Japan,stimulates nodulation by Rhizobium huakuii bv.renge." Jouranl of Fermentation and Bioengineering. 77. 630-635 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nobuhiko NOMURA: "Characterization and nucleotide sequence of the region reqired for the replication a non-self transmissible plasmid pEC3 isolated from Erwinia carotovora subsp.carotovora" Journal of Fermentaion and Bioengineering. 78. 250-254 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hyon-Ju CHO: "Hormonal control of morphogenesis and tissue competency for shoot organogenesis in Astragalus sinicus(Chinese Milk Vetch)." Culture Letter. in press (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.AKAO: "Rhizobium host-range extension by intro-duction of Rhizobium trifolii nod genes." Soil Science and Plant Nutrition. 40. 703-708 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shiro HIGASHI: "Forest-restoration effects at Alnus hirsuta(Spach)Rupr.plantations on tow small islands in northern Japan." Report of Faculty of Science,Kagoshima University. 27(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.UCHIUMI: "Nodule formation by clover-Rhizobium carrying chromosomal nod genes." Journal of Gen.Appl.Microbiol.41. 9-20 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akio INOUE: "Function of DRAT and DRAG and the trans-criptional regulation of draTG operon in Azospirillum lipoferum." Abstruct of 4th Annual Meeting for Plant-Microbe Interaction. 4. 82-84 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takashi OZAWA: "Concomitant inhibition of nitrogen fixation and N-phenyl-1-naphtylamine uptake activity of bacteroids of a Bradyrizobium japonicum strain by nitrate." Soil Science and Plant Nutrition. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshikatsu MUROOKA: "Recombinant Microbes for Industrial and Agricultural Applications" Marcel Dekker,Inc.,New York, 877 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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