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Joint Study on Evolution of Skeletal Muscle E-C Coupling

Research Project

Project/Area Number 06044163
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionTokushima University

Principal Investigator

INOUE Isao  Associate Professor, Institute for Enzyme Research Tokushima University, 酵素科学研究センター, 助教授 (80001973)

Co-Investigator(Kenkyū-buntansha) CHRACHRI Abdesslam  Research Fellow, Marine Biological Association of UK, 研究員
WILLIAMSON Roddy  Advanced Research Fellow, Marine Biological Association of UK, 上級研究員
BROWN Euan r  Advanced Research Fellow, Marine Biological Association of UK, 上級研究員
BONE Quentin  Research Director, Marine Biological Association of UK, 部長
KUKITA Fumio  Research Associate, National Institute for Physiological Sciences, 生体膜, 助手 (40113427)
TSUTSUI Izuo  Research Associate, National Institute for Physiological Sciences, 生体膜, 助手 (80202183)
YAMAGISHI Shunnichi  Professor, National Institute for Physiological Sciences, 生体膜, 教授 (70014032)
ABBOTT N.Joa  ロンドン大学キングス校, 副教授
BROWN R.Euan  プリムス海洋生物研究所, 研究員
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥9,900,000 (Direct Cost: ¥9,900,000)
Fiscal Year 1995: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1994: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsSkeletal muscle / E-C coupling / Calcium ion / Evolution / DHP-receptor / Cephalochordate / Agnathan / Nifedipine / Nerve
Research Abstract

A distinctive feature of the excitation-contraction coupling (E-C coupling) of skeletal muscle in higher vertebrates in that Ca^<2+> -influx is not necessary for contraction. This is because membrane depolarisation directly triggers Ca^<2+> release from the sarcoplasmic reticulum (SR). The molecule responsible for this signal transduction in the transverse tubular (T-tubular) membrane is a dihydropyridine (DHP) receptor, which is thought to be responsible for the dual function, of voltage sensor (underlying E-C coupling) and the L-type Ca^<2+> channel. In E-C coupling in invertebrate muscle fibers, influx of external Ca^<2+> is necessary for contraction, and a DHP -receptor is present, but responsible for only the L-type Ca^<2+> channel function. Therefore, the E-C coupling mechanism in higher vertebrates is qualitativele different from that in invertebrates. There must have been an evolutionary step to acquire the novel depolarisation-induced Ca^<2+> release mechanism. The main object … More ive of the present research project is to examine species representing different stages in animal evolution, and compare the biophysical and morphological properties of E-C coupling.
We reported that the evolutionry step might have occurred in lower chordates between amphioxus (Branchiostoma lanceolatum) and lamprey (Lampetra planeri). Whole-cell voltage clamp experiments on single fast muscle fibers suggest that this evolutionary change is accompanied by the appearance of a component of intramembrane charge movement, which is blocked by nifedipine, a DHP derivative, and by a remarkable slowing-down of the activation kinetics of the Ca^<2+> channel. In the fibers of lamprey, mouse, and dogfish, intracellular Ca^<2+>-release (measured using the Ca^<2+> sensitive flurescence dye fluo3) is associated with membrane depolarisation, is resistant to externally applied Co^<2+>, and is suppressed by nifedipine. In contrast, in the amphioxus fiber, Ca^<2+>-release is suppressed by Co^<2+>. The results strongly support the hypothesis that nifedipine-sensitive intramembrane charge movement is the signal tansduction mechanism of vertebrate skeletal muscle E-C coupling.
Marine invertebrates and lower vertebrates represent a unique 'gene-bank' of such diverse material and should provide us with chronological evidence about the molecular evolution of the DHP-receptor. Less

Report

(2 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report

Research Products

(42 results)

All Other

All Publications (42 results)

  • [Publications] Chrachri A.: "Ionic currents in primary sensory hair cells dissociated from the statocytes of squid" Soc.Neurosci.Abst.25. 168- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Brown E.R.: "Coupling between Schwann cells from the squid giant axon" Proc.Poy.Soc.Lond.B. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Brown E.R.: "Dihydropyridine sensitive chargemovement in isolated crayfish neurons." J.Physiol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Williamson R.: "Ionic currents in secondary sensory hair cells isolated from the statocytes of squid and cuttlefish" J.Comp.Physiol.A.177. 261-271 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Williamson R.: "The sensory bases for orientation in cephalopods" J.Marine.Boil.Assoc.U.K.75. 83-92 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kishimoto U.: "The detection and properties of electronic Na^+ and K^+ transport in the squid axon membrane In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 53-72 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inoue I.: "Resting and active K+ channels in the squiod axon membrane In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 73-84 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamagishi S.: "The effects of inaternal Ca^<2+> and Mg^<2+> on ion channels in the squid giant axon In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 153-160 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abbott N.J.: "Electrophysiology of squid Schwann cells In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 197-212 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Pichon Y.: "Periaxonal ion regulation in the squid In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 229-244 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Bone O.: "The structure and physiology of cehalopod muscle fibres In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 301-330 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nicholson C.: "Diffusion properties of the microenvironment of cephalopod brain In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 383-398 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Buderlmann B.U.: "Cephalopod brains : promissing preparation for brain physiology In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 399-414 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Williamson R.: "The statocysts of cephalopod In “Cephalopod Neurobioloty"" Oxford University Press,Oxford, 503-520 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inoue I.: "Evolution of skeletal muscle excitation-contraction coupling In “Toward Molecular Biology of Ion Channels"" Elsevier Scientific Inc.Amsterdai (in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inoue I.: "A phylogenetic to understanding the molecular mechanism of striated muscle excitation-contraction coupling. In “Basic Neuroscience in Invertebrates"" Japan Scientific Societies Press,Tokyo (in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chrachri, A.: "Ionic currents in primary sensory hair cells dissociated from the statocytes of squid" Soc.Neurosci, Abst.25. 168 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Brown, E.R.: "Coupling between Schwann cells from the squid giant axon" Proc.Roy.Soc.Lond.B. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Brown, E.R.: "Dihydropyridine sensitive charge-movement in isolated crayfish neurons." J.Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Williamson, R.: "Ionic currents in secondary sensory hair cells isolated from the statocytes of squid and cuttlefish" J.Comp.Physiol.A. 177. 261-271 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Williamson, R.: "The sensory bases for orientation in cephalopods" J.Marine Boil.Assoc.UK.75. 83-92 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kishimoto, U.: Oxford University Press, Oxford. The detection and properties of electronic Na^+ and K^+ transport in the squid axon membrane. In "Cephalopod Neurobioloty", 53-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inoue, I.: Oxford University Press, Oxford. Resting and active K^+ channels in the squiod axon membrane. In "Cephalopod Neurobioloty", 73-84 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamagishi, S.: Oxford University Press, Oxford. The effects of internal Ca^<2+> and Mg^<2+> on ion channels in the squid giant axon. In "Cephalopod Neurobioloty", 153-160 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abbott, N.J.: Electrophysiology of squid Schwann cells In "Cephalopod Neurobioloty"25HC04 : Oxford University Press, Oxford, 197-212 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Pichon, Y.: Oxford University Press, Oxford. Periaxonal ion regulation in the squid In "Cephalopod Neurobioloty", 229-244 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Bone, Q.: Oxford University Press, Oxford. The structure and physiology of cephalopod muscle fibers In "Cephalopod Neurobioloty", 301-330 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nicholson, C.: Oxford University Press, Oxford. Diffusion properties of the microenvironment of cephalopod brain In "Cephalopod Neurobioloty", 383-398 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Buderlmann, B.U.: Oxford University Press, Oxford. Cephalopod vrains : promissing preparation for brain physiology In "Cephalopod Neurobioloty", 399-414 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Williamson, R: Oxford University Press, Oxford. The statocysts of cephalopod In "Cephalopod Neurobioloty", 503-520 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inoue, I: Elsevier Scientific Inc.Amsterdam (in press). Evolution of skeletal muscle excitation-contraction coupling In "Toward Molecular Biology off Ion Channels",

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Inoue, I.: Japan Scientific Societies Press, Tokyo (in press). A phylogenetic approach to understanding the molecular mechanism of striated muscle excitation-contraction coupling. In "Basic Neuroscience in Invertebrates",

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Minami,K: "Regulation of a non-selective cation channel of cultured porcine coronary artery smooth muscle cells by tyrosine kinase" Pflugers Arch.426. 254-257 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Inoue,I.: "Evolution of skeletal muscle excitation-contraction coupling and the appearance ofdihydropyridine-sensitive intramembrane charge movement" Proc.Roy Soc.Lond B.255. 181-187 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Beauvais,F.: "Anti-IgE induces the opening of non-selective cation channels on human basophils Fundam.Clin.Phrmacol." Fundam.Clin.Phrnacol.8. 246-250 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Inoue,I.: "Chloride channel as acetylcholine receptor in buccal muscle cell of Aplysia" Jap.J.Physiol.44. 149-155 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagahama,T: "Acetylcholine-activated two types of ion channels prduce hyperpolarisation or depolarisation at different blocks of buccal myscle in Aplysia" J.Physiol.(Lond). (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Inoue,I.: "ATP-sensitive K_+ channel of the inner membrane of rat liver mitochondria “Handbook of Membrane Channels:Molecular and Cellular Physiology" Academic press,New York, 549-554 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Inoue,I.: "Evolution of skeletal muscle excitation-contraction coupling “Towards Molecular Biophysics of Ion Channels"" Elsevier Scientific Inc.Amsterdam(in press),

    • Related Report
      1994 Annual Research Report
  • [Publications] Inoue,I.: "Resting and active K channels in the squid axon membrane Cephalopod Neurobiology" Oxford University Press,Oxford (in press),

    • Related Report
      1994 Annual Research Report
  • [Publications] Kishimoto,U.: "Voltage dependence of the ouabain-sensitive current of the squid axon membrane Cephalopod Neurobiology" Oxford University Press,Oxford (in press),

    • Related Report
      1994 Annual Research Report
  • [Publications] Pichon,Y.: "Periaxonal K_+ accumulation and regulation in the squid Cephalopod Neurobiology" Oxford University Press,Oxford (in press),

    • Related Report
      1994 Annual Research Report

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Published: 1994-03-31   Modified: 2016-04-21  

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