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Use of Bombyx mori Mutants to Study on Insect Biochemistry

Research Project

Project/Area Number 06044175
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

FUJII Hiroshi  FACULTY OF AGRICULTURE・KYUSHU UNIVERSITY・ASSOCIATE PROFESSOR, 農学部, 助教授 (10038268)

Co-Investigator(Kenkyū-buntansha) LAW Jhon h  ARIZONA UNIVERSITY PROFESSOR, 教授
WELLS Michael a  ARIZONA UNIVERSITY・PROFESSOR, 教授
TSUCHIDA Kozo  NATIONAL INSTITUTE HEALTH・RESEARCHER, 主任研究官 (40231435)
MAEKAWA Hideaki  NATIONAL INSTITUTE HEALT・HEAD, 室長 (60100096)
SUMITA Motoyuki  KYOTO INSTITUTE TECHNOLOGY・ASSOCIATE PROFESSOR, 繊維学部, 助教授 (50127164)
KOBAYASHI Masahiko  TOKYO UNIVERSITY・PROFESSOR, 農学部, 教授 (60162020)
SUZUKI Akinori  TOKYO UNIVERISITY・PROFESSOR, 農学部, 教授 (90011907)
DOIRA Hiroshi  KYUSHU UNIVERSITY・PROFESSOR, 農学部, 教授 (30038210)
LAW John H.  アリゾナ大学, 生化学部・評議員, 教授
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥18,100,000 (Direct Cost: ¥18,100,000)
Fiscal Year 1995: ¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 1994: ¥9,400,000 (Direct Cost: ¥9,400,000)
KeywordsBombyx mori / CBP / YI and Y+^I midgut / Cnad+^C silkgland / LTP / membrane permeability / immunocytology / immuno-gold / electron microscop / 突然変異系統 / カロチノイド透過性 / 人口試料 / cDNAライブラリー / カロチノイド結合タンパク質
Research Abstract

Wells lab isolated lutein bound protein (40kDa) from the midgut of silkworm. Fuji also isolated and characterized carotinoid binding proitein (600kDa) from the midgut of the Y+^I silkworm. This protein is identical with CBP in hemolymph on the bisis of immunological similarity and the same biochemical properties as to CBP.The midgut of the YI silkworm contained a yellow colored protein, but did not in the Y+^I silkworm. The color pigment was carotinoid. But the protein did not react with anti-CBP antibody. Hence it is a new carotinoid binding protein other than CBP.
cDNA library from the midgut of the Y+^I silkworm was constracted. cDNA encoding CBP has been cloned by using anti-CBP antibody and several clones were obtained. Two clones were analyzed nucleotide sequence, but they were not identical to CBP.
Immunocytochemical analysis of carotinoid binding protein (CBP) in the midgut and middle silkgland of silkworm, Bombyx mori. Immunogold technique showed that CBP was located in rough ER,and in the small vesicle associated with smooth ER.Carotinoid was secreated into hemolymph from midgut as carotinoid bound CBP complex by exocytosis, incorporated into the middle part of silkgland from hemolymph as the complex and finally tansferrd to sericin prior to excretion of sericine particles.
A lipid transfer particles (LTP) has been purified from the hemolymph of Bombyx mori. the LTP has three apoproteins (79% of particles mass) apo LTP-I,apoLTP=IInd apoLTP=III,Mr=350,000,85,000and 60,000. The lipid of LTP is phosphalipid (54%), monoacylgicerol (8.3%), diaacylglycerol (17%), free fatty acid (14.3%), triacylglycerol (5.9%) and hydrocarbon (0.4%).This protein is able to catalyze the transfer of a number of lipids among different lipid particle.

Report

(2 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 藤井 博: "カイコ非膠着卵変異体の粘液腺の発育に伴う蛋白質と核酸の変動" 九大農学芸誌. 49. 61-66 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiwara Haruhiko: "Molecular genetic characterization of chromosomal fragment in genetic mosaic strains of Bombyx mori by chorion RFLP analysis." Chromosoma. 103. 468-474 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuchida Kozo: "Determing gene location on chromosomes of Bombyx mori using fluorescence in situ hybridization." Appl.Entmol.Zoo.,. 30. 225-230 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ziegler R.: "Apolipopholine-III and adipokinetic hormone in lipid metabolism of larval Manduca sexta." Insect Biochem.Molec Biol.,. 21. 101-108 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Maekawa Hideaki: "A non-LTR retrotransposon is dispersed throughout the genome of silkworms." J.Cell.Biochem.21A. 200 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuchida Kozo: "Lipophorin and lipid transfer protein of Bombyx mori." J.Cell.Biochem.,. 21A. 220 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujii Hiroshi: "Changes of protein and nucleic acid synthesis in the developing colleterial glands of the silkworm, Bombyx mori." Sci.Bull.Fac.Agr., Kyushu Univ.49. 61-66 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuiwara Haruhiko: "Molecular genetic characterization of chromosomal fragment in genetic mosaic strains of Bombyx mori by chorion RFLP analysis." Chromosoma. 103. 468-474 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuchida Kozo: "Determing gene location on chromosomes of Bombyx mori using fluorescence in situ hybridization." Appl.Entmol.Zoo.30. 225-230 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ziegler R.: "Apolipopholine-III and adipokinetic hormone in lipid metabolism of larval Manduca sexta." Insect Biochem.Molec Biol.21. 101-108 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Maekawa Hideaki: "A non-LTR retrotransposon is dispersed throughout the genome of silkworms." J.Cell.Biochem.21A. 200 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuchida Kozo: "Lipophorin and lipid transfer protein of Bombyx mori." J.Cell.Biochem.21A. 200 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujii Hiroshi: "Haemolymph protein profiles in allatectomy-induced trimolters resembie those of a recessive trimolting mutant of the silkwworm,Bombyx mori" Comp.Biochem.Physiol.,. 107B. 579-584 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sumida Motoyuki: "Sucrose activity and its kinetic properties in peritrophic membrane,and in membrane-bound and soluble fractions of midgut in the silkworm,Bombyx mori" Comp.Biochem.Physiol.,. 108A. 255-264 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sumida Motoyuki: "Purification and some properties of soluble beta-fructofuranosidase from larval midgut of the silkworm,Bombyx mori" Comp.Biochem.Physiol.,. 107B. 273-284 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Michael A.Wells: "Cloning and sequencing of the early trypsin gene from Ades aegypti." Insect Moiec.Biol.,. in press. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Michael A.Wells: "cDNA sequence of an elastase-like protein from the midgut of larval Manduca sexta." Insect Molec.Biol.,. in press. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小林正彦: "ポリメラーゼ連鎖反応による飼育残沙を含めた大量試料からの核多角体病蚕の検出" 日本蚕糸学雑誌. 63. 392-398 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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