Molecular cloning and analysis of requlatory-strucutral genes for arginine biosynthtic pathway in Bacillus amyloliquefaciens
| Project/Area Number |
06044248
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| Research Category |
Grant-in-Aid for Overseas Scientific Survey.
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| Allocation Type | Single-year Grants |
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YAMANE Kunio Institute of Biological Sciences, University of Tsukuba, Professor, 生物科学系, 教授 (20013336)
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| Co-Investigator(Kenkyū-buntansha) |
KYUNG Hee Mi Dept. of Biology. Sookmyung Women's Univer, Professor
NAKAMURA Kouji Institute of Biological Sciences, University of Tsukuba, Lectural Professor, 生物科学系, 講師 (40212097)
MIN Kyung-Hee Dept.of Biology, Sookmyung Women's University, Professor
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| Project Period (FY) |
1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Keywords | Arg Genes / Arginine Biosynthetic Pathway / Bacillus Amyloliquefaciens |
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| Research Abstract |
As a model system to compare with those of Escherichia coli, the structure of the arg genes and their molecular genetic property in Bacillus amyloliquefaciens will be investigated. In the case of E.coli, other genes except for the argECBH cluster for the biosynthesis of arginine from glutamate are not tightly linked on the chromosome. Their expressions are also requlated by the product of unlinked gene, argR.The structure of the arg genes and the operon in E.coli has been studied by many researchers. Although the study on the arg auxotroph and the biosynthesis of arginine have been done a little in other microorganisms, molecular genetics of arg genes in Bacillus sp.remains to be elucidated. In professor Min's laboratory the argEC genes and the arg operon in Corynebacterium glutamicum has been cloned and studied. Therefore, the arg genes in Bacillus amyloliquefaciens will be cloned by a complementation analysis of E.coli arg auxotropic mutant. After the nucleotide sequence analysis, the gene organization and requlation of the arg operon in the B.amyloliquefaciens chromosome will be compared with those of E.coli and Corynebacterium system.
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Research Products
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