| Project/Area Number |
06044270
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| Research Category |
Grant-in-Aid for Overseas Scientific Survey.
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| Allocation Type | Single-year Grants |
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| Research Institution | Ocean Research Institute, University of Tokyo |
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Principal Investigator |
TAKEI Yoshio university of Tokyo, Ocean Research Insitute, Associate professor, 海洋研究所, 助教授 (10129249)
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| Co-Investigator(Kenkyū-buntansha) |
BALMENT Rich マンチェスター大学, 生物科学部, 教授
HAZON Neil セント, アンドリュース大学・ガッティー臨海実験所, 講師
HAMANO Kaoru National Research Institute of Fisheries Science, Research Associate, 中央水産研究所, 農林水産技官
HYODO Susumu University of Tokyo, College of arts and Sciences, Instructor, 教養学部, 助手 (40222244)
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| Project Period (FY) |
1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Keywords | Angiotensin / Endothelin / ANP / vasotocin / osmoregulation / eel / rainbow trout / flounder |
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| Research Abstract |
Takei visited Dr.Balment of Manchester University to conduct following experiments using flounder.
(1) Papaverine, a vasorelaxant drug, was injected into the flounder, and the time course of drinking rate, plasma angiotensin II and vasotocin levels were measured. The effect of angiotensin II inhibition on drinking rate and plasma vasotocin level after papaverine was also examined.
(2) We sequenced eel atrial natriuretic peptide (ANP) in eels for the first time in fishes. Synthetic eel ANP was injected into European eels, and the time course of change in drinking rate and plasma vasotocin level was examined.
2. Hyodo also visited Dr.Balment and conducted the following experiments.
(1) He found that the expression of vasotocin mRNA decreased after transfer of rainbow trout to seawater, and Dr.Balment found that plasma vasotocin level in the rainbow trout increased after transfer to seawater. Thus, they worked together to conduct similar experiments in the flounder.
(2) The time course of change in vasotocin mRNA expression in the paraventricular nucleus of the trout brain was examined by Northern blot analysis.
3. Hamano visited Dr.Hazon of University of St.Andrews and conducted the following experiments.
(1) She had worked with Dr.Hazon previously for the vasopressor effect of angiotensin II in the dogfish, and found that mechanism of the pressor effect was quite different from that of other animals. She further analyzed the mechanism using an isolated aortic ring of dogfish.
(2) She also examined the vasorelaxant activity of homologous C-type natriuretic peptide using isolated aortic ring of dogfish.
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