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Analysis of the cross-talk mechanisms in biological systems

Research Project

Project/Area Number 06045035
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionKagawa Medical University

Principal Investigator

IRINO Shozo  Kagawa Medical University・President, 学長 (50033056)

Co-Investigator(Kenkyū-buntansha) WAISMAN David M  University of Calgary・Professor, 医学部, 教授
TAMAOKI Taiki  University of Calgary・Professor, 医学部, 教授
SMITH Eldon  カルガリ大学, 医学部長
YAMAGUCHI Fuminori  Kagawa Medical University・Research Associate, 医学部, 助手 (40271085)
TOKUDA Masaaki  Kagawa Medical University・Associate Professor, 医学部, 助教授 (10163974)
TAKAHARA Jiro  Kagawa Medical University・Professor, 医学部, 教授 (00033085)
TAKEUCHI Yoshiki  Kagawa Medical University・Professor, 医学部, 教授 (20116619)
HATASE Osamu  Kagawa Medical University・Professor, 医学部, 教授 (50033220)
HOSOKAWA Kiyoshi  Kagawa Medical University・Vice President, 副学長 (70093698)
ELDON Smith  University of Calgary・Dean
細見 弘  香川医科大学, 医学部, 教授 (70030864)
WANG Jerry H  カルガリ大学, 医学部, 教授
村上 哲英  香川医科大学, 医学部, 教授 (20032873)
宇多 弘次  香川医科大学, 副学長 (80107044)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsCdk5 / p35 / calcineurin / Ca / Calmodulin kinases / calcium / Lymphocyte / Cartilage / Brain / Retina / Cyclin-dependent kinase 5 / アルツハイマー病 / calmodulin kinase-IV / リン酸化 / 細胞内情報伝達 / クロストーク / パーキンソン病 / カルモジュリンキナーゼIV / 白血病細胞 / Cdk 5 / アルツハイマー脳 / MARCKS / カルモジュリン依存性リン酸化酵素IV
Research Abstract

In the nervous system, we found the novel regulatory factor p35 which is related to cyclins and controls cyclin-dependent kinase 5 (Cdk5). In the Alzheimer is brain, both Cdk 5 and p35 were shown to in crease in amount. We also demonstrated that Tau protein could cause the tangle formation observed in the senile plaques of Alzheimer is deisease by phosphorylating Cdk5/p35. The distribution of Cdk5 and p35 in the nervous system was examined, and both were shown to exist in Purkinje cells but not in granular cells. Both were present in retina, especially in the inner nuclear layr and ganglion cells. p39, an isoform of p35, was also present in retina.
In the normal white blood cells (WBC) and leukemic WBC,cross-talk mechanisms were compared. Especially, the phosphorylation level of the intrinsic proteins was much higher in the leukemic WBC than the normal WBC.We showed the intracellular calcium level was 20-30% higher in leukemic WBC than normal WBC,and the amount of calcium/calmodulin-dependent protein kinase IV (CaMK-IV) was increased also in leukemic WBC.
CaMK-IV was shown to be expressed during the initial atages of the chondrogenesis, especially in the proliferating zone, but not in the later stages. This indicates that CaMK-IV is actively involved in the chodrogenesis during the initial differentiation of immature chondrocytes. The substrate proteins have been analyzed.
The simultaneous analysis of CaMK-I,-II and IV and calcineurin (CaN), a calcium/calmodulin-dependent phosphatase, was performed in the rat hippocampus. We successfully demonstrated that CaMK-I and -IV as well as already reported CaMK-II and CaN were expressed in the hippocampus. The change in the amount of these enzymes in the Alzheimer is brain has been analysing.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] Osamu Hatase et al.: "Neural transplantation approaches for the treatment of epilepsy." Epilepsia. 36. 269 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuya Masada et al.: "Protective effect of vagus nerve stimulation on forebrain ischaemia in gerbil hippocampus." NeuroReport. 7. 446-448 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuhito Tomizawa et al.: "Localization and developmental changes in the neuron-specific Cdk5 activator (P35nck5a) in the rat brain." Neuroscience. 74. 519-529 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuhiko Toyoshima et al.: "Expression of calbindin-D28K by reactive astrocytes in gerbil hippocampus after ischaemia." NeuroReport. 7. 2087-2091 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hironobu Morita et al.: "Fos induction in rat brain neurons following stimulation of the hepatoportal Na-sensitive mechanism." American Journal of Physiology. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuyuki Hirooka et al.: "Reticalmin : A novel calcium/calmodulin-dependent Protein kinase IV like protein in rat retina." Vision Research. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuyuki Hirooka et al.: "Developmental alteration of the expression and kinase activity of cyclin-dependent kinase 5 (Cdk5)/p35nck5a in the rat retina." Journal of Neurochemistry. 67. 2478-2483 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Bushra Yaqoob Ahmed et al.: "A chronological study of the expression of glial fibrillary acidic protein and calbindin-D28K by reactive astrocytes in the electrically lesioned rat brain." Neuroscience Research. 26. 271-278 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiyoshi Moriwaki et al.: "Immunosuppressant FK506 prevents mossy fiber sprouting induced by kindling stimulation." Neuroscience Research. 25. 191-194 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuya Masada et al.: "Embryonic transplantation and ischemic memory deficit." Neuroscience Research. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masaaki Tokuda et al.: "Involvement of calmodulin-dependent protein kinases I and IV in long term potentiation." Brain Research. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Osamu Hatase et al.: "Neural transplantation approaches for the treatment of epilepsy." Epilepsia. 36. 269 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuya Masada et al.: "Protective effect of vagus nerve stimulation on forebrain ischaemia in gerbil hippocampus." NeuroReport. 7. 446-448 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuhito Tomizawa et al.: "Localization and developmental changes in the neuron-specific Cdk5 activator (P35nck5a) in the rat brain." Neuroscience. 74. 519-529 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuhiko Toyoshima et al.: "Expression of calbindin-D28K by reactive astrocytes in gerbil hippocampus after ischaemia." NeuroReport. 7. 2087-2091 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hironobu Morita et al.: "Fos induction in rat brain neurons following stimulation of the hepatoportal Na-sensitive mechanism." American Journal of Physiology. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuyuki Hirooka et al.: "Reticalmin : A novel calcium/calmodulin-dependent Protein kinase IV like protein in rat retina." Vision Research. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuyuki Hirooka et al.: "Developmental alteration of the expression and kinase activity of cyclin-dependent kinase 5 (Cdk5) /p35nck5a in the rat retina." Journal of Neurochemistry. 67. 2478-2483 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Bushra Yaqoob Ahmed et al.: "A chronological study of the expression of glial fibrillary acidic protein and calbindin-D28K by reactive astrocytes in the electrically lesioned rat brain." Neuroscience Research. 26. 271-278 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiyoshi Moriwaki et al.: "Immunosuppressant FK506 prevents mossy fiber sprouting induced by kindling stimulation." Neuroscience Research. 25. 191-194 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuya Masada et al.: "Embryonic transplantation and ischemic memory deficit." Neuroscience Research. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masaaki Tokuda et al.: "Involvement of calmodulin-dependent protein kinases I and IV in long term potentiation." Brain Research. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuhito Tomizawa et al.: "Localization and developmental changes in the neuron-specific Cdk5 activalor (P35nck5a) in the rat brain." Neuroscience. 74・2. 519-529 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tetsuhiko Toyoshima et al.: "Expression of calbindin-D28K by reactive astrocytes in gerbil hippocampus after ischaemia." NeuroReport. 7・13. 2087-2091 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuyuki Hirooka et al.: "Reticalmin : A novel calcium/calmodulin-dependent protein kinase IV like protein in rat retina." Vision Research. (in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuyuki Hirooka et al.: "Developmcntal alteration of the expression and kinase activity of cyclin-depenent kinase 5 (Cdk5)/p35nck5a in the rat retina." Journal of Neurochemistry. 67・6. 2478-2483 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Bushra Yaqoob Ahmed et al.: "A chronological study of the expression of glial fibrillary acidic protein and calbindin-D28K by reactive astrocytes in the electrically lesioned rat brain." Neuroscience Research. 26・3. 271-278 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akiyoshi Moriwaki et al.: "Immunosuppressant FK506 prevents mossy fiber sprouting induced by kindling stimulation." Neuroscience Research. 25. 191-194 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tetsuya Masada et al.: "Embryonic transplantation and ischemic memory deficit." Neuroscience Research. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masaaki Tokuda et al.: "Involvement of calmodulin-dependent protein kinases I and IV in long term potentiation." Brain Research. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hajime Nishio,et al.: "pp60^<c-src> expression in rat spermatogenesis." Biochem.Biophys.Res.Commun.206(2). 502-510 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masayuki Matsushita,et al.: "Developmental changes of cyclin dependent kinase 5 subcellular localization in the rat cerebrellum." NeuroReport. 6(9). 1267-1270 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsutomu Masaki,et al.: "Increase in src-related protein tyrosine kinases in human liver cirrhosis and hepatocellular carcinoma." Internatl.Hepatol.Commun.4. 54-60 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takaya Gotoh,et al.: "Identification of rapl as a target for the Crk SH3 domain-binding guanine nucleotide-relasing factor C3G." Mol.Cell.Biol.15(2). 6146-6153 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsutomu Masaki,et al.: "Enhanced expression of the protein kinase substrate annexin I in human hepatocellular carcinoma." Hepatology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuhito Tomizawa,et al.: "Localization and developmental changes in the neuron-specific Cdk5 activator (P35 nck 5a) in the rat brain." Neuroscience. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujisawa,Mutsuo: "Mutual interaction between host and graft tissues in the embryonic neural transplantion." NeuroReport. 5. 805-808 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Moia,Lizomar de J.M.P.: "Immunosuppressants and calcineurin inhibitors,cyclosporin A and FK506 inhibit epileptogenesis of amydgaloid kindling rat in reversible manner." Brain Research. 648. 337-341 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohnishi,Makoto: "Changes in annexins I and II Ievels during tne postnatal development of rat pancreatic islets." Journal of Cell Science. 108. 2117-2125 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masaki,Tsutomu: "Involvement of annexin I and annexin II in hepatocyte proliferation:Can annexins I and II be markers for proliferative hepatocytes?" Hepatology. 20. 425-435 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miyamoto,Kazuhiro: "In situ localization of rat testis-specific calcineurin B subunit isoform β1 in the developing rat testis." Biochemical and Biophysical Research Communications. 203. 1275-1283 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hosokawa,Jiro: "Morphological changes in the hippocampus in amygdaloid kindled mouse." Epilepsy Research. 20. 11-20 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tomizawa,Kazuhito: "Developmental alteration and neuron-specific expression of bone morphogenetic protein(BMP)-6 mRNA in rodent brain." Molecular Brain Research. 28. 122-128 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nisho,Hajime: "pp60^<c-Src> exptession in rat spermatogenesis." Biochemical and Biophysical Research Communications. 206. 502-510 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohnishi,Makoto: "Involvement of annexin I in glucose-induced insulin secretion in rat pancreatic islets." Endcrinology. 印刷中.

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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