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天然超分子化学の総合的研究

Research Project

Project/Area Number 06240106
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionOsaka University

Principal Investigator

楠本 正一  大阪大学, 大学院・理学研究科, 教授 (30028253)

Co-Investigator(Kenkyū-buntansha) 小倉 協三  東北大学, 反応化学研究所, 教授 (80006303)
三川 潮  富山医科薬科大学, 薬学部, 教授 (60012613)
橘 和夫  東京大学, 大学院・理学系研究科, 教授 (70142081)
杉浦 幸雄  京都大学, 化学研究所, 教授 (40025698)
山田 靜之 (山田 静之)  名古屋大学, 大学院・理学研究科, 教授 (90022540)
Project Period (FY) 1994 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥110,300,000 (Direct Cost: ¥110,300,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 1995: ¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 1994: ¥89,200,000 (Direct Cost: ¥89,200,000)
Keywords超分子 / 構造認識 / 生物活性 / 天然有機化合物 / DNA / 脂質 / 糖質 / 蛋白質
Research Abstract

生物活性天然有機化合物が示す多様な作用は、いずれもそれらの化合物がまず生体側の分子によって特異的に構造を認識され、超分子というべき複合体を形成することに始まる。本研究は天然有機化合物による生物機能発現の最初の段階にあたる超分子の形成過程と機能発現の機構を有機化学的に明らかにすることを目指して平成6〜8年度の3年間にわたって実施した重点領域研究「天然超分子の化学」の研究成果を取りまとめるために行ったものである。
比較的低分子量の有機分子から核酸、脂質、糖質、蛋白質にいたる広範な天然有機化合物を対象とした研究成果を整理し、領域研究に参画した多数の研究者の今後の研究の展開に活用するとともに、これを広く公表して関連分野の発展に資することを目指して以下の活動を行った。すなわち、6月に大阪で「成果取りまとめシンポジウム」を公開で開催し、各班から選ばれた6名が領域を代表して3年間の研究を中心に講演した。この際、できるだけ全班員の参加を得るために、交付を受けた予算から班員の旅費を支出した。このシンポジウムの機会に各班長がそれぞれの研究成果の概要を集め、取りまとめの方針を検討する資料とした。またこの資料を中心として研究終了ヒアリングの説明資料およびその際に提出する報告書を作成した。そこには新聞報道などを通じて社会的に与えたインパクトも紹介するよう務めた。この報告書は各班の主な成果の概要をわかりやすくまとめ、各研究者の主な発表論文を収録して情報源としての価値を高めたもので、これを領域ニュースとしても全員に配付した。続いて個々の3年間の研究成果について各班員から和文4、英文2ページの要旨を集めて、和英の研究成果報告書を印刷し、これを広く配付した。

Report

(4 results)
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] H.Sone: "Isolation and stereostructure of dolasatin I,a cytotoxic cyclic hexapeptide from the Japanese sea hare Dolabella" Tetrahedron. 53. 8149-8154 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Nagaoka: "Distinct phosphate backbone contacts revealed by some mutant peptides of zinc finger protein Spl" Biochemistry. 35. 8761-8768 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Morii: "Cooperative oligomerization enhances sequence-selective DNA binding by a short peptide." J.Am.Chem.Soc.5. 124-128 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] G.K.Paul: "Chemical structures of amphidinols 5 and 6 isolated from marine dinoflagellate amphidinium klebsii and their cholesterol-" J.Mar.Biotechnol.5. 124-128 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Oikawa: "New Efficient Synthesis of a Biosynthetic Precursor of Lipid A." Bull.Chem.Soc.Jpn.70'6. 1435-1440 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Morita: "Molecular Cloning of Pea cDNA Encoding Cycloartenol Synthase Its Functional Expression in Yease." Biol.Pharm.Bull.20. 770-775 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Okuno: "Interation of C-1027 chromophore with d(GTATAC)_2:A binding model based on NMR experiments." J.Am.Chem.Soc.118. 4729-4730 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Takahashi: "DNA cleavage by a nine-membered masked enediyne,an analogue of the kedarcidin and C-1027 chromophores." Angew.Chem.Int.Ed.Engl.35. 1835-1837 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Konoki: "Blockade of MTX-induced Ca^<2+> influx in rat glioma C6 cells by alkylamines." J.Natural Tosins. 5. 209-217 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Sasaki: "The complete structure of maitotoxin,part I;configuration of the C1-C14 side chain." Angew.Chem.,Int.Ed.Engl.35. 1672-1675 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Nonomura: "Complete structure of Maitotoxin,part II;configuration of C135-C142 side chain and absolute configuration of the entire" Angew.Chem.,Int.Ed.Engl.35. 1675-1678 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ushio Sankawa: "cDNA and genomic DNA cloning of chalcone synthase from pueraria lobata." Biol.Pharm.Bull.19. 71-76 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuo Suda: "Separation of wild-type lipopolysaccharides by centrifugal partition chromatoqraphy:isolation of smooth form species." J.Endotoxin Res.3・1. 1-7 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koichi Fukase: "Synthetic study of a bioactive ^3H-labeled analogue of lipid A." Synlett. 1996・3. 252-254 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Sekljic: "Synthesis of neoglycoproteins containing 5-O-phosphorylated kdomonosaccharide,4-O and 5-O- phospholylated" J.Endotoxin Res.3. 151-164 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koichi Fukase: "A novel method stereoselective glycosidation with thioglycosides:promotion by hypervalent iodine reagents" Tetrahedron. 52・11. 3897-3904 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Saito: "Novel actin depolymerizing macrolide aplyronine A." J.Biochem.120・3. 552-555 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Suenaga: "Isolation and stereostructure of aurilide,a novel cyclodepsipeptide from the Japanese Sea Hare Dolabella" Tetrahedron Lett.37・37. 6771-6774 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ushio Sankawa: "Cloning of chalcone-flavanone isomerase cDNA from pueraria lobta and Its overexpression in Escherichia coli." Protein Expression and Purification. 8. 183-190 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ushio Sankawa: "Cloning of the polyketide synthase gene at X fro aspergillus terreus and its identification as the 6-methylsalicylic acid" Mol.Gen.Genet.253. 1-10 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koichi Fukase: "Synthesis of an Analog of Biosynthetic Precursor Ia of Lipid A by an Improved Method: A Novel Antagonist Containing" Tetrahedron Lett.36. 7455-7458 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Koichi Fukase: "Synthetic Route for ^<14>C-labeling of a Bioactive Lipid A Analogue." Tetrahedron Lett.36. 8645-8648 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Makoto Ojika: "Dolastian E, a New Cyclic Hexapeptide Isolated form the Sea Hare Dolabella auricularia." Tetrahedron Lett.36. 5057-5058 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Makoto Ojika: "Dolabelides A and B,Cytotoxic 22-Membered Macrolides Isolated from the Sea Hare Dolabella auricularia" Tetrahedron Lett.36. 7491-7494 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tetsuya Kusakabe: "Sequence Preference for Strand Cleavage of Gapped Duplexes by Dynemicin A: Possible Mechanism of Sequence-Dependent" Biochemistry. 34. 9944-9950 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masayuki Kusano: "Molecular cloning of cDNA encoding rat 2,3-oxidosqualene: lanosterol cyclase" Biol.Pharm.Bull.18. 195-197 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Koichi Fukase: "Synthesis and purification by HPLC of des-O-acyl partial structures of Escherichia coil Re lipopolysaccharide." J.Endotoxin Res.1. 149-163 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuo Suda: "Difference of macrophage activation in response to S-and R-form lipopolysaccharides(LPS) separated by centrifugal partition" Biochem.Biopys.Res.Commun.in press (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Ojika: "Absolute Stereochemistry of Aplyronine A,a potent Antitumor Substance of Marine Origin." J.Am.Chem.Soc.116. 7441-7442 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Okuno: "Computer modeling analysis for enediyne-chromophore-apoprotein complex of macromolecular antitumor antibiotic C-1027." J.Med.Chem.37. 2266-2273 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takuyuki Matsumoto: "Alterations of binding mode and cutting site by G I replacement in preferred cleavage sequence 5'-agg of chromoprotein C-1027." Biochem.Biopys.Res.Commun.205. 1533-1538 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masayuki Kusano: "Molecular cloning of cDNA encoding rat 2,3-oxidosqualene:lanosterol cyclase" Biol.Pharm.Bull.18. 195-197 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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