造血幹細胞の増殖におけるMybインヒビターMomの役割

• Project/Area Number: 06277222
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: 石井 俊輔 理化学研究所, 分子遺伝学研究室, 主任研究員 (00124785)
• Project Period (FY): 1994
• Project Status: Completed (Fiscal Year 1994)
• Budget Amount: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 1994: ¥2,700,000 (Direct Cost: ¥2,700,000)
• Keywords: 造血幹細胞 / Myb / シグナル伝達

Research Abstract

未分化造血細胞の増殖に必須のMybは一群の遺伝子の転写を活性化する。標的遺伝子のひとつであるmim-1遺伝子の活性化にはMybと別の転写因子NF-IL6(C/EBPβ)が協調して作用する必要がある。私達はMybとDNA複合体の立体構造を決定し、MybのDNA結合ドメインの表面に疎水性領域が存在し、この領域を介してMybはNF-IL6と相互作用することを明らかにした。このように造血細胞増殖に関与する転写因子間の相互作用が原子レベルで理解されるようになってきた。

Research Products

• [Publications] Kanei-Ishii,C.: "C-Myb-induced trans-activation mediated by heat shock elements without sequence-specific DNA binding of c-Myb." J.Biol.Chem.269. 15768-15775 (1994)
• [Publications] Ogata,K.: "Solution structure of a specfic DNA complex of the Myb DNA-binding domain with cooperative recognition helices." Cell. 79. 639-648 (1994)
• [Publications] Terasawa,H.: "Structure of the N-terminal SH3 domain of GRB2 complexed with a peptide from the guanine nucleotide releasing factor Sos." Nature Structural Biology. 1. 891-897 (1994)
• [Publications] Ogata,K.: "Solution structures of the c-Myb DNA-binding domain:comparison between the free and DNA-complxed forms." Nature Structural Biology. 2(in press.). (1995)
• [Publications] Mizuguchi,G.: "c-Myb repression of c-erbB-2 trancription by direct binding to the c-erbB-2promoter." J.Biol.Chem.270(in press.). (1995)
• [Publications] Tashiro,S.: "Cell type-specific trans-activation by the B-myb gene product requirement of the putative cofactor binding to the C-terminal conserved domain." Oncogene. (in press.). (1995)