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Mechanism of Intracellular Signaling via Ras Protein

Research Project

Project/Area Number 06280218
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionKobe University School of Medicine

Principal Investigator

KATAOKA Tohru  Kobe Univ.Sch.Med., Dept.Physiology II, Professor, 医学部, 教授 (40144472)

Co-Investigator(Kenkyū-buntansha) OKADA Tomoyo  Kobe Univ.Sch.Med., Dept.Physiology II, Instructor, 医学部, 助手 (80294205)
HU Chang-deng  Kobe Univ.Sch.Med., Dept.Physiology II, Instructor, 医学部, 助手 (70294204)
KATAOKA Yuriko  Kobe Univ.Sch.Med., Dept.Physiology II, Instructor (50233739)
KARIYA Ken-ichi  Kobe Univ.Sch.Med., Dept.Physiology II, Associate Prof. (40263371)
Project Period (FY) 1994 – 1999
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥41,300,000 (Direct Cost: ¥41,300,000)
Fiscal Year 1999: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1998: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1997: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1996: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1995: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1994: ¥7,000,000 (Direct Cost: ¥7,000,000)
Keywordsras Oncogene / Posttranslational Modification / Farnesylation / Phospholipase C / Adenylyl Cyclase / Adenylyl Cyclase-Associated Protein / raf Oncogene / rap1 Anti-Oncogene / GTP結合蛋白質 / raplA癌抑制遺伝子 / フォスフォリパーゼC / rap1A癌抑制遺伝子 / 合成ペプチド / Rasがん遺伝子 / Rap1A / がん抑制遺伝子 / エフェクター / rafがん遺伝子 / 線虫遺伝学 / Raf蛋白質 / アクチン結合蛋白質 / 中心体 / 細胞質分裂 / rasがん遺伝子 / 出芽酵母 / 線虫 / ゲルゾリン
Research Abstract

1. We discovered a novel interaction between Ras and its effectors ; human Raf and yeast adenylyl cyclase. This "second" interaction is essential for full activation of the effectors by Ras, and dependent on the "activator" region and the posttranslational modification (famesylation) of Ras. The second Ras-binding sites are located in the cysteine-rich domain of Raf and in a complex between cyclase-associated protein CAP and the adenylyl cyclase C-terminus. Based on these findings, we elucitated the molecular mechanisms by which the anti-oncogene product Rap1 antagonizes the Ras function, by which Ras and Rap1 exert differential regulatory activities toward Raf-1 and B-Raf, and by which phosphorylation of Rap1 by A-kinase results in inhibition of its Ras-antagonizing activity. We proposed that the strength of the second interaction is a critical determinant of effector regulation by Ras family small G-proteins.
2. By screening many single amino acid substitution mutants of H-Ras for interaction with various Ras effector proteins, we demonstrated that significant differences exist in the recognition mechanisms by which the multiple effector proteins associate with Ras, and obtained H-Ras mutants that could discriminate the effectors. Using these mutants, we examined which effector was really involved in some Ras-induced cellular events.
3. We discovered a novel Ras-effector candidate PLC-ε in nematode and isolated its human homologue. The human PLC-ε exhibited GTP-dependent binding to Ras/Rap1, and, when coexpressed with Ras and Rap1, was translocated from the cytoplasm to the plasma membrane and the Golgi apparatus, respectively, and had its phospholipase C activity greatly activated. Thus, PLC-ε is a real Ras effector. In addition, we discovered two novel proteins RA-GEF-1 and-2, which possessed both a RA domain associating directly with the GTP-bound Rap1 and a GEF domain catalysing GDP-GTP exchange on Rap1.

Report

(7 results)
  • 2000 Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (78 results)

All Other

All Publications (78 results)

  • [Publications] Minato,T., et al.: "Quantitative analysis of the mutually competitive binding of human Raf-1 and yeast adenylyl cyclase to Ras proteins"J.Biol.Chem.. 269. 20845-20851 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masuda,T., et al.: "Protein kinase Byr2 is a target of Ras1 in fission yeast Schizosaccharomyces pombe"J.Biol.Chem.. 270. 1979-1982 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hu,C.-D., et al.: "Cysteine-rich region or Raf-1 interacts with activator domain of post-translationally modified H-Ras"J.Biol.Chem.. 270. 30274-30277 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okada,T., et al.: "Post-translational modification of H-Ras is required for activation of, but not for association with, B-Raf"J.Biol.Chem.. 271. 4671-4678 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akasaka,K., et al.: "Differential Structural requirements for interaction of Ras protein with its distinct downstream effectors"J.Biol.Chem.. 271. 5353-5360 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shinkai,M., et al.: "Difference i n the mechanism of interaction of Raf-1 and B-Raf with H-Ras"Biochem.Biophys.Res.Comm.. 223. 729-734 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shima,F., et al.: "Effect of association with adenylyl cyclase-associated protein on the interaction of yeast adenylyl cyclase with Ras protein"Mol.Cell.Biol.. 17. 1057-1064 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okazaki,M., et al.: "Synergistic activation of c-fos proinoter activity by Raf and Ral GDP dissociation stimulator"Oncogene. 14. 515-521 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yanagihara,C., et al.: "Association of elongation factor 1α and ribosomal protein L3 with the proline-rich region of yeast adenylyl cyclase-associated protein CAP"Biochem.Biophys.Res.Comm.. 232. 503-507 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hu,C-D., et al.: "Coassociation of Rap1A and Ha-Ras with Raf-1 N-terminal region interferes with Ras-dependent activation of Raf-1"J.Biol.Chem.. 272. 11702-11705 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tamada,M., et al.: "Membrane recruitment of Raf-1 is not the only function of Ras i n Raf-1 activation"Oncogene. 15. 2959-2964 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shibatohge,M., et al.: "Identification of PLC210, a Caenorhabditis elegans phospholipase C, as a putative effector of Ras"J.Biol.Chem.. 273. 6218-6222 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shirouzu,M., et al.: "Interactions of the amino acid residue at position 31 of the c-Ha-Ras protein with Raf-1 and RalGDS"J.Biol.Chem.. 273. 7737-7742 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohnishi,M., et al.: "Selective inhibition of Ras interaction with its particular effector by synthetic peptides corresponding to the Ras effector region"J.Biol.Chem.. 273. 10210-10215 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kim,J.H., et al.: "Activation of Ras and its downstream extracellular signal-regulated protein kinases by the CDC25 homology domain of mouse Son-of-sevenless 1 (mSos1)"Oncogene. 16. 2597-2607 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishida,Y., et al.: "Coiled-coil interaction of N-terminal 36 residues of cyclase-associated protein with adenylyl cyclase is sufficient for its function in Saccharomyces cerevisiae Ras pathway"J.Biol.Chem.. 273. 28019-28024 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watari,Y., et al.: "Identification of Ce-AF-6, a novel Caenorhabditis elegans protein, as a Ras putative effector"Gene. 224. 53-58 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hu,C-D., et al.: "Effect of phosphorylation on activities of Rap1A to interact with Raf-1 and to suppress Ras-dependent Raf-1 activation"J.Biol.Chem.. 274. 48-51 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Goshima,M., et al.: "Characterization of a novel Ras-binding protein Ce-FLI-1 comprising leucine-rich repeats and gelsolin-like domains"Biochem.Biophys.Res.Comm.. 257. 111-116 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okada,T., et al.: "The strength of interaction at the Raf cysteine-rich domain is a critical determinant of response of Raf to Ras family small GTPases"Mol.Cell.Biol.. 19. 6057-6064 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka,Y., et al.: "H-Ras signals to cytoskeletal machinery in induction of integrin-mediated adhesion of T cells"J.Immunol.. 163. 6209-6216 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Liao,Y., et al.: "RA-GEF, a novel Rap1A guanine nucleotide exchange factor containing a Ras/Rap1A-associating domain, is conserved between nematode and humans"J.Biol.Chem.. 274. 37815-37820 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shima,F., et al.: "Association of yeast adenylyl cyclase with cyclase-associated protein CAP forms a second Ras-binding site which mediates its Ras-dependent activation"Mol.Cell.Biol.. 20. 26-33 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kataoka,T., et al.: "Cytoskeleton and G-proteins in the regulation of cancer"Noboru Kuzumaki. 184 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Minato et al.: "Quantitative analysis of the mutually competitive binding of human Raf-1 and yeast adenylyl cyclase to Ras proteins"J.Biol.Chem.. 269. 20845-20851 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Masuda et al.: "Protein kinase Byr2 is a target of Ras1 in fission yeast Schizosaccharomyces pombe"J.Biol.Chem.. 270. 1979-1982 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C-D.Hu et al.: "Cysteine-rich region of Raf-1 interacts with activator domain of post-translationally modified H-Ras"J.Biol.Chem.. 270. 30274-30277 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Okada et al.: "Post-translational modification of H-Ras is required for activation of, but not for association with, B-Raf"J.Biol.Chem.. 271. 4671-4678 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Akasaka et al.: "Differential structural requirements for interaction of Ras protein with its distinct downstream effectors"J.Biol.Chem.. 271. 5353-5360 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Shinkai et al.: "Difference in the mechanism of interaction of Raf-1 and B-Raf with H-Ras"Biochem.Biophys.Res.Comm. 223. 729-734 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] F.Shima et al.: "Effect of association with adenylyl cyclase-associated protein on the interaction of yeast adenylyl cyclase with Ras protein"Mol.Cell.Biol.. 17. 1057-1064 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Okazaki et al.: "Synergistic activation of c-fos promoter activity by Raf and Ral GDP dissociation stimulator"Oncogene. 14. 515-521 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C.Yanagihara et al.: "Association of elongation factor 1α and ribosomal protein L3 with the proline-rich region of yeast adenylyl cyclase-associated protein CAP"Biochem.Biophys.Res.Comm.. 232. 503-507 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C-D.Hu et al.: "Coassociation of Rap1 A and Ha-Ras with Raf-1 N-terminal region interferes with Ras-dependent activation of Raf-1"J.Biol.Chem.. 272. 11702-11705 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Tamada et al.: "Membrane recruitment of Raf-1 is not the only function of Ras in Raf-1 activation."Oncogene. 15. 2959-2964 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Shibatohge et al.: "Identification of PLC210, a Caenorhabditis elegans phospholipase C, as a putative effector of Ras."J.Biol.Chem.. 273. 6218-6222 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Shirouzu et al.: "Interactions of the amino acid residue at position 31 of the c-Ha-Ras protein with Raf-1 and RalGDS."J.Biol.Chem.. 273. 7737-7742 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Ohnishi et al.: "Selective inhibition of Ras interaction with its particular effector by synthetic peptides corresponding to the Ras effector region."J.Biol.Chem.. 273. 10210-10215 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] J.H.Kim et al.: "Activation of Ras and its downstream extracellular signal-regulated protein kinases by the CDC25 homology domain of mouse Son-of-sevenless 1 (mSos 1)"Oncogene. 16. 2597-2607 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Nishida et al.: "Coiled-coil interaction of N-terminal 36 residues of cyclase-associated protein with adenylyl cyclase is sufficient for its function in Saccharomyces cerevisiae Ras pathway."J.Biol.Chem.. 273. 28019-28024 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Watari et al.: "Identification of Ce-AF-6, a novel Caenorhabditis elegans protein, as a Ras putative effector"Gene. 224. 53-58 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C-D.Hu et al.: "Effect of phosphorylation on activities of Rap 1 A to interact with Raf-1 and to suppress Ras-dependent Raf-1."J.Biol.Chem.. 274. 48-51 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Goshima et al.: "Characterization of a novel Ras-binding protein Ce-FLI-l comprising leucine-rich repeats and gelsolin-like domains"Biochem.Biophys.Res.Comm.. 257. 111-116 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Okada et al.: "The strength of interaction at the Raf cysteine-rich domain is a critical determinant of response of Raf to Ras family small GTPases"Mol.Cell.Biol.. 19. 6057-6064 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Tanaka et al.: "H-Ras signals to cytoskeletal machinery in induction of integrin-mediated adhesion of T cells"J.Immunol.. 163. 6209-6216 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Liao et al.: "RA-GEF, a novel Rap 1 A guanine nucleotide exchange factor containing a Ras/Rap 1 A-associating domain, is conserved between nematode and humans"J.Biol.Chem.. 274. 37815-37820 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] F.Shima et al.: "Association of yeast adenylyl cyclase with cyclase-associated protein CAP forms a second Ras-binding site which mediates its Ras-dependent activation"Mol.Cell.Biol.. 20. 26-33 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Goshima, M., et al.: "Characterization of a novel Ras-binding protein Ce-FLI-1 Comprising leucine-rich repeats and gelsolin-like domains"Biochem. Biophys. Res. Comm.. 257. 111-116 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Okada, T., et al.: "The strengh of interaction at the Raf cysteine-rich domain is a critical determinant of response of Raf to Ras family small GTPases"Mol. Cell. Biol.. 19. 6057-6064 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanaka, Y., et al.: "H-Ras signals to cytoskeletal machinery in induction of integrin-mediated adhesion of T cells"J. Immunol.. 163. 6209-6216 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Liao, Y., et al.: "RA-GEF, a novel Rapl A guanine nucleotide exchange factor containing a Ras/Rapl A-associating domain, is conserved between nematode and humans"J. Biol. Chem.. 274. 37815-37820 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shima, F., et al.: "Association of yeast adenylyl cyclase with cyclase-associated protein CAP forms a second Ras-binding site which mediates its Ras-dependent activation"Mol. Cell. Biol.. 20. 26-33 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohnishi,M.,et al.: "Selective inhibition of Ras interaction with its particular effector by synthetic peptides corresponding to the Ras effector region." J.Biol.Chem.273巻17号. 10210-10215 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kim,J.H.,et al.: "Activation of Ras and its downstream extracellular signal-regulated protein kinases by the CDC25 homology domain of mouse Son-of-sevenless 1(mSosl)." Oncogene. 16巻20号. 2597-2607 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishida,Y.,et al.: "Coiled-coil interaction of N-terminal 36 residues of cyclase-associated protein with adenylyl cyclase in sufficient for its function in Saccharomyces cerevisiae Ras pathway." J.Biol.Chem.273巻43号. 28019-28024 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watari,Y.,et al.: "Identification of Ce-AF-6,a novel Caenorhabditis elegans protein, as a putative Ras effector." Gene. 224巻1-2号. 53-58 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hu,C-D.,et al.: "Effect of phosphorylation on activities of RaplA to interact with Raf-1 and to suppress Ras-dependent Raf-1 activation." J.Biol.Chem.274巻1号. 48-51 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Goshima,M.,et al.: "Characterization of a novel Ras-binding protein Ce-FLI-1 comprising leucine-rich repeats and gelsolin-like domains." Biochem.Biophys.Res.Comm.未定(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hu,C-D., et al.: "Coassociation of Rap1A and Ha-Ras with Raf-1 N-terminal region interferes with Ras-dependent activation of Raf-1" J.Biol.Chem.272巻. 11702-11705 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tamada,M., et al.: "Membrane recruitment of Raf-1 is not the only function of Ras in Raf-1 activation." Oncogene. 15巻. 2959-2964 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shibatohge,M., et al.: "Identification of PLc210, a Caenorhabditis elegans Phospholipase C, as a putative effector of Ras." J.Biol.Chem.273巻. 6218-6222 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shirouzu,M., et al.: "Interactions of the amino acid residue at position 31 of the c-Ha-Ras protein with Raf-1 and Ral-GDS." J.Biol.Chem.273巻(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohnishi,M., et al.: "Selective inhibition of Ras interaction with its particular effector by synthetic peptides corresponding to the Ras effector region." J.Biol.Chem.273巻(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Shinkai et al.: "Difference in the mechanism of interaction of Raf-1 and B-raf with H-Ras." Biochem.Biophys.Res.Comm.223巻3号. 729-734 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] F.Shima et al.: "Effect of association with adenylyl cyclase-associated protein on the interaction of yeast adenylyl cyclase with Ras protein." Mol.Cell.Biol.17巻3号. 1057-1064 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Kariya et al.: "C.elegans homologs of ralGDS,AF-6,Cdc25 and phospholipase Cβ interact with Let-60." Worm Breeder's Gazette. 14巻5号. 34-35 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Okazaki et al.: "Synergistic activation of c-fos promoter activity by Raf and Ral GDP dissociation stimulator." Oncogene. 14巻3号. 515-521 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] C-D. Hu et al.: "Coassociation of Rap1A and Ha-Ras with Raf-1 N-terminal region interferes with Ras-dependent activation of Raf-1." J.Biol.Chem.272巻(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] C.Yanagihara et al.: "Association of elongation factor 1α and ribosomal protein L3 with the proline-rich region of yeast adenylyl cyclase-associated protein." Biochem.Biophys.Res.Comm.233または234巻(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Masuda et al.: "Protein kinase Byr2 is a target of Rast in the fission yeast Schizosaccharomyces pombe." J. Biol. Chem.270. 1979-1982 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Kariya et al.: "The novel Ras-binding protein Ach-1 is associated with cetrosomes." Worm Breeder's Gazette. 14. 78-79 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] C.-D. Hu et al.: "Cystein-rich region of Rail-1 interacts with activator domain of post-translationally modified Ha-Ras." J. Biol. Chem.270. 30274-30277 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Okada et al.: "Post-translational modification of H-Ras is required for activation of, but not for association with, B-Raf." J. Biol. Chem.271. 4671-4678 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Akasaka et al.: "Differential structural requirements for interaction of Ras protein with its distinct downstream effectors." J. Biol. Chem.271. 5353-5360 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 苅谷研一他: "出芽酵母のRAS" 実験医学(増刊). 12. 259-260 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Minato et al.,: "Quantitative analysis of mutually competitive binding of human Raf-1 and yeast adenylyl cyclase to Ras proteins." J.Biol.Chem.269. 20845-20851 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Masuda et al.,: "Protein kinase Byr2 is a target of Ras1 in the fission yeast Schizosaccharomyces pombe." J.Biol.Chem.270. 1979-1982 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Yamawaki-Kataoka et al.,: "A novel candidate of Ras-target protein in Caenorhabditis elegans with homology to the actin-severing protein family." Mol.Cell.Biol.,. 15(発表予定). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2019-02-15  

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