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New Generation of Protein Crystallography

Research Project

Project/Area Number 06303018
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIKI Kunio  Kyoto Univ.Prof., 大学院・理学研究科, 教授 (10116105)

Co-Investigator(Kenkyū-buntansha) TOYOSHIMA Chikashi  Tokyo Univ.Prof., 分子細胞生物学研究所, 教授 (70172210)
TSUKIHARA Tomitake  Osaka Univ.Prof., 蛋白質研究所, 教授 (00032277)
FUKUYAMA Keiichi  Osaka Univ.Prof., 理学部, 教授 (80032283)
HIGUCHI Yoshiki  Kyoto Univ.Assoc.Prof., 大学院・理学研究科, 助教授 (90183574)
TANAKA Isao  Hokkaido Univ.Prof., 大学院・理学研究科, 教授 (70093052)
渡辺 信久  高エネルギー物理学研究所, 助手 (70212321)
山根 隆  名古屋大学, 工学部, 助教授 (80030055)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 1995: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1994: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsProtein Crystallography / Structural Biology / X-ray Structure Analysis / Supramacromolecular Complex / Synchrotron Radiation / シンクロトロン放射学 / シンクロトロン放射光
Research Abstract

We investigated the target and problems in the following five categories shown below in the field of protein crystallography and related methodology. The problems were extensively discussed in the symposium and the internal meeting.
1) Development and improvement of data collection and structure determination.
2) New aspects on the relationship between the three-dimensional structure and the function.
3) Survey of the target macromolecules for the structure determination.
4) New development of the usage of synchrontron radiation.
5) Cooperation with related method in the structure determination of biological macromolecules.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] H. Nishida: "Crystal Structure of NADH-Cytochrome b_5 Reductase from Pig Liver at 2.4 Å Resolution" Biochemistry. 34. 2763-2767 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Suzuki: "Crystal Structure of the Macrophage Migration Inhibitory Factor from Rat Liver" Nature Struct. Biol. 3. 259-266 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Higuchi: "Location of Active Sites of NiFe Hydrogenase Determined by the Combination of Multiple Isomorphous Replacement and Multiwavelength Anomalous Diffraction Methods" Acta Crystallogr.D50. 781-785 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N. Kunishima: "Pentacoordination of the Heme Iron of Arthromyces ramosus Peroxidase Shown by a 1.8 Å Resolution Crystallographic Study at pH 4.5" FEBS Lett.378. 291-294 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Tsukihara: "Structure of Metal Sites of Oxidized Bovine Heart Cytohrome c Oxidase at 2.8 Å" Science. 269. 1069-1074 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] C. Toyoshima: "The Structure of the R-type Straight Flagellar Filament of Salmonella at 9 Å Resolution by Elertron Cryomicroscopy" J. Mol. Biol.249. 69-87 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Nishida: "Crystal Structure of NADH-Cytochrome b_5 Reductase from Pig Liver at 2.4 Resolution" Biochemistry. 34. 2763-2767 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Suzuki: "Crystal Structure of the Macrophage Migration Inhibitory Factor from Rat Liver" Nature Struct.Biol. 3. 259-266 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Higuchi: "Location of Active Sites of NiFe Hydrogenase Determined by the Combination of Multiple Isomorphous Replacement and Multiwavelength Anomalous Diffraction Methods" Acta Crystallogr.D50. 781-785 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Kunishima: "Pentacoordination of the Heme Iron of Arthromyces ramosus Peroxidase Shown by a 1.8 Resolution Crystallographic Study at pH 4.5" FEBS Lett.378. 291-294 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Tsukihara: "Structure of Metal Sites of Oxidized Bovine Heart Cytochrome c Oxidase at 2.8" Science. 269. 1069-1074 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] C.Toyoshima: "The Structure of the R-type Straight Flagellar Filament of Salmonella at 9 Resolution by Electron Cryomicroscopy" J.Mol.Biol. 249. 69-87 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H. Nishida: "Crystal Structure of NADH-Cytochrome b_5 Reductase from Pig Liver at 2.4 A Resolution" Biochemistry. 34. 2763-2767 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M. Suzuki: "Cystal Structure of the Macrophage Migration Inhibitory Factor from Rat Liver" Nature Struct. Biol. 3. 259-266 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y. Higuchi: "Location of Active Sites of NiFe Hydrogenase Determined by the Combination of Multiple Isomorphous Replacement and Multiwavelength Anomalous Diffraction Methods" Acta Crystallogr.D50. 781-785 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] N. Kunishima: "Pentacoordination of the Heme Iron of Arthromyces ramosus Peroxidase Shown by a 1.8 Å Resolution Crystallographic Study at pH 4.5" FEBS Lett.378. 291-294 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Tsukihara: "Structure of Metal Sites of Oxidized Bovine Heart Cytochrome c Oxidase at 2.8 Å" Science. 269. 1069-1074 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] C. Toyoshima: "The Structure of the R-type Straight Flagellar Filament of Salmonella at 9 Å Resolution by Electron Cryomicroscopy" J. Mol. Biol.249. 69-87 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N.Katayama et al.: "Preliminary X-ray Crystallographic Studies of Photosynthetic Reaction Center from a Thermophilic Sulfur Bacterium,Chromatium tepidum" FEBS Lett.348. 158-160 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Saika et al.: "Crystallization of Mutant β Subunit of F_1-ATPase from Thermophilic Bacillus PS3" J.Mol.Biol.242. 709-711 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Kita et al.: "Crystal Structure Determination of a Flavoprotein FP_<390> from a Luminescent Bacterium,Photobacterium phosphoreum" J.Biochem.117. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Nishda et al.: "Crystal Structure of NADH-Cytochrome b_5 Reductase from Pig Liver at 2.4 A Resolution" Biochemistry. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.Saito et al.: "Crystallization and Preliminary X-ray Crystallographic Analysis of Chitosanase from Bacillus circulans MH-K1" Acta Crystallogr.Section D. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Inaka et al.: "Crystal Structure of a Gultathionylated Mutant Human Lysozyme:A Folding Intermediate Mimic in the Formation of a Disulfide Bond" Acta Crystallogr.Section D. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 三木邦夫(分担): "錯体化学-基礎と最近の話題" 講談社, 220 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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