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Studies on the Molecular Mechanisms That Produces and Controls Flagellar and Ciliary Movements

Research Project

Project/Area Number 06304006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field 動物生理・代謝
Research InstitutionUniversity of Tokyo

Principal Investigator

KAMIYA Ritsu  University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (10124314)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Munenori  Toyoma University Faculty of Science, Associate Professor, 理学部, 助教授 (30019004)
OGAWA Kazuo  National Institute for Basic Biology, Associate Professor, 基礎生物学研究所, 助教授 (30132731)
BABA Akitsugu  Ochanomizu Women's College, Faculty of Science, Professor, 理学部, 教授 (80011691)
TAKAHASHI Keiichi  International Christian Univ. Dept. of General Education, Professor, 教養学部, 教授 (40011481)
MOHRI Hideo  National Institure for Basic Biology, Director General, 基礎生物学研究所, 所長 (70012268)
能村 堆子  お茶の水女子大学, 名誉教授 (30022578)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥21,600,000 (Direct Cost: ¥21,600,000)
Fiscal Year 1996: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1995: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1994: ¥12,200,000 (Direct Cost: ¥12,200,000)
Keywordscilia / flagella / dynein / microtubule / calcium / cyclic AMP / cell movement / sperm / チューブリン / 滑り運動 / 燐酸化 / リン酸化 / 運動調節
Research Abstract

This research has been aimed at the elucidation of the molecular mechanism that generates and controls flagellar and ciliary movements. The beating of flagella and cilia is based on the sliding movement between adjacent microtubules, driven by dynein arms. Through examination of the structure of dynein arms and properties of the sliding movement, we obtained the following important findings : 1) Outer dynein arms in multicellular organisms always contain two heavy chains, whereas those in unicellular organisms have three heavy chains ; 2) The three intermediate chains in the outer arm dynein have enzyme activities involved in nucleotide metabolism ; 3) Inner arm dynein contains true actin as a subunit ; 4) The activity of dynein function may be regulated through nucleotide binding to multiple sites in the dynein heavy chain ; 5) Dynein itself may have a nature to produce oscillatory movements. 6) Outer arm dynein and inner arm dynein appears to have different properties of force production. Regarding the regulation of ciliary and flagellar movements, we examined the effect of protein phosphorylation and calcium ion. We first obtained evidence that the site of phosphorylation-based regulation in mammalian sperm fagella is located at their bases ; protein phosphorylation at the base appears to regulate whether the flagella initiate beating. In Paramecium cilia, Phosphorylation appears to modulate the sensitivity to calcium. Thus, ciliary movements appear to be regulated by the coordination of calcium ion and protein phosphorylation. In beating sea urchin sperm axonemes, microtuble sliding is apparently regulated by calcium ion. However, the velocity of microtubule sliding under load-free conditions is not affected by calcium. Thus, calcium ion appears to regulate the force generation, possibly by regulating the number of active dynein arms. How calcium regulates the activity of dynein remains an important future problem.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report

Research Products

(144 results)

All Other

All Publications (144 results)

  • [Publications] Yagi,T.: "Nanometer-scale vibration in mutant axonemes of Chlamydomonas" Cell Motility and the Cytoskeleton. 29. 177-185 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada,S.: "Functional reconstitution of Chlamydomonas outer dynein arms from α-β and γ subunits : requirement of a third factor" Journal of Cell Biology. 126. 737-745 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Muto,E.: "Immunological detection of actin in the 14S ciliary dynein of Tetrahymena" FEBS Letters. 343. 589-595 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida,M.: "Calcium and cyclic AMP mediate sperm activation,but Ca alone contributes sperm chemototaxis in the ascidian,Ciona savignyi" Develop Growth and Differentiation. 36. 589-595 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin,Z.X.: "Monoclonal antibodies against the protein complex that contains the flagellar movement-initiating phosphoprotein of Oncorhynchus keta" Journal of Biochemistry. 115. 885-890 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morisawa,M.: "Cell signalling mechanisms for sperm motility" Zoological Science. 11. 647-662 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin,Z.X.: "Isolation and properties of a protein complex that contains flagellar movement-initiating phosphoprotein from testes of a white salmon" Journal of Biochemistry. 115. 552-556 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mimori,Y.: "Extrusion of rotating microtubules on the dynein-track from a microtubule-dynein γ-complex" Cell Motility and the Cytoskeleton. 30. 180-191 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mimori,Y.: "ATP-induced sliding of microtubules on tracks of 22S dynein molecules aligned with the same polarity" Cell Motility and the Cytoskeleton. 27. 180-191 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoki,F.: "Analysis of flagellar bending in hyperactivated hamster and mouse spermatozoa" Journal of Reproduction and Fertility. 101. 397-403 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Minoura,I.: "Strikingly different propulsive forces generated by different dynein deficient Chlamydomonas mutants in viscous media" Cell Motil.Cytoskeleton. 31. 130-139 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yagi,T.: "Novel mode of hyper-oscillation in the paralyzed axoneme of a Chlamydomonas mutant lacking the central-pair microtubules" Cell Motil.Cytoskeleton. 31. 207-214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogawa,K.: "Interspecies conservation of outer arm dynein intermediac chain sequences defines two intermediate chain subclasses" Molec.Biol.Cell. 6. 685-696 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kamiya,R.: "Exploring the function of inner and outer dynein arms with Chlamydomonas mutants" Cell Motil.Cytoskeleton. 32. 98-102 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura,S.: "Assembly and function of Chlamydomonas flagellar mastigonemes as probed with a monoclonal antibody" J.Cell Sci.109. 57-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shingyoji,C.: "Effect of beat frequency on the velocity of microtubule sliding in reactivated sea urchin sperm flagella under imposed head vibration." J.Exp.Biol.198. 645-653 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shingyoji,C.: "Cyclic bending movements induced locally by successive iontophoretic application of ATP to an elastase-treated flagellar axoneme." J.Cell Sci.108. 1359-1369 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shingyoji,C.: "Flagellar quiescence response in sea urchin sperm induced by electric stimulation" Cell Motil.Cytoskel.31. 59-65 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi,K.: "Mechanisms of flagellar motility probed with microtechniques" Cell Motil.Cytoskel.32. 110-113 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimizu,T.: "Comparison of the motilie and enzymatic properties of two microtubule minus-end motors,ncd and cytoplasmic dynein" Biochemistry. 34. 1575-1582 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura,K.: "Proteolysis of tubulin isotypes within Tetrahymena axonemes" Biol.Chem.Hoppe Seyler. 372. 729-732 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takaya,C.: "Comparison of axonemal proteins from two kinds of Tetrahymena" Comp. Biochem. Physiol.112B. 727-732 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada,Y.: "Effects of neurotransmitters on ciliary beating of sea urchin plutei" Physiol.Zool.68. S48 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mohri,H.: "Outer arm dynein of sperm flagella and cilia in the animal kingdom" Memoires du Museum National D'Histoire Naturelle. 166. 15-22 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Oda,S.: "Sperm-activating proteins from unfertilized eggs of the Pacific herring,Clupea Pallasii" Develop.Growth & Differ.37. 257-261 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takai,H.: "Change in intracellular K^+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts" J.Cell Sci.108. 1175-1181 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Togo,T.: "Existence of three mechanisms for blocking polyspermy in oocytes of the mussel Mytilus edulis" Biol.Bull.189. 330-339 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kinoshita,S.: "Regulatory role of nucleotides in axonemal function" Cell Motil.Cytoskeleton. 32. 46-54 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Si,Y.: "Activation of mammalian sperm motility by regulation of microtubule sliding via cyclic adenosine 5'-monophosphate-dependent phosphrylation" Biol.Reprod.53. 1081-1087 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Si,Y.: "Extrusion of microtubule doublet outer dense fibers 5-6 associating with fibrous sheath sliding in mouse sperm flagella" J.Exp.Zool.273. 355-362 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Omoto,C.K.: "Ability of paralyzed flagella mutants of Chlamydomonas to move" Cell Motility and the Cytokeleton. 33. 88-94 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugase,Y.: "Cloning and characterization of the actin-encoding gene of Chlamydomonas reinhardtii" Gene. 168. 117-121 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada,S.: "Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer-doublet microtubules" Cell Motility and the Cytoskeleton. 36. 68-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshimura,K.: "Conversion of beating mode in Chlamydomonas flagella induced by electric stimulation." Cell Motility and the Cytoskeleton. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamori,M.: "Structure and function of the pore canals of the sea urchin madreporite" Phil.Trans.Roy.Soc.Lond.B.351. 659-676 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noguchi,M.: "Effects of organic solvents on the coupling between ATP hydrolysis and sliding of microtubules in axonemes from Chlamydomonas flagella" Journal of Eukaryotic Microbiology. 44. 127-133 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogawa,K.: "Is outer arm dynein intermediate chain 1 multifunctional?" Molecular Biology of the Cell. 7. 1895-1907 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishijima,S.: "Calcium-dependent bidirectional power stroke on the dynein arms in sea urchin sperm" Journal of Cell Science. 109. 2833-2842 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogawa,K.: "A dynein motor superfamily" Cell Structure and Function. 21. 343-349 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imafuku,Y.: "Fluctuation in the microtubule sliding movement driven by kinesin in vitro" Biophysical Journal. 70. 878-886 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Edamatsu,M.: "Isolation and characterization of pos mutants defective in correct positioning of septum in Shizosaccharomyces pombe" Zoological Science. 13. 235-239 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Minamikawa,S.: "Acquisition,initiation and maintenance of sperm motility in the shark Trikias sehcha" Comparative Biochemistry and Physiology. 113. 387-392 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba,K.: "Identification of tubulins associated with the 950 kDa protease from salmon sperm" Biomedical Research. 17. 87-93 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Togo,T.: "Aminopeptidase-like protease released from oocytes suppresses acrosome reaction of sperm and established polyspermy block in oocytes of the mussel" Developmental Biology. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada,Y.: "Modification of ciliary beating in sea urchin larvae induced by neurotransmetters : beat-plane rotation and control of frequency fluctuation." Journal of Experimental Biology. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yagi, T.: "Nanometer-scale vibration in mutant axonemes of Chlamydomonas" Cell Motil. Cytoskel. 29. 177-185 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada, S.: "Functional reconstitution of Chlamydomonas outer dynein arms from alpha-beta and gamma subunits : requirement of a third factor" J.Cell Biol.126. 737-745 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Muto, E.: "Immunological detection of actin in the 14S ciliary dynein of Tetrahymena" FEBS Lett.343. 173-176 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida, M.: "Calcium and cyclic AMP mediate sperm activation, but Ca alone contributes sperm chemototaxis in the ascidian, Ciona savignyi" Devel. Growth Differ. 36. 589-595 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin, Z.X.: "Monoclonal antibodies against the protein complex that contains the flagellar movement-initiating phosphoprotein of Oncorhynchus keta" J.Biochem.115. 885-890 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morisawa, M.: "Cell signalling mechanisms for sperm motility" Zool. Sci.11. 647-662 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin, Z.X.: "Isolation and properties of a protein complex that contains flagellar movement-initiating phosphoprotein from testes of a white salmon" Journal of Biochemistry. 115. 552-556 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mimori, Y.: "Extrusion of rotating microtubules on the dynein-track from a microtubule-dynein gamma-complex" Cell Motil. Cytoskel.30. 180-191 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mimori, Y.: "ATP-induced sliding of microtubules on tracks of 22S dynein molecules aligned with the same polarity" Cell Motil. Cytoskel.27. 180-191 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoki, F.: "Analysis of flagellar bending in hyperactivated hamster and mouse spermatozoa" J.Repr. Fertil.101. 397-403 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Minoura, I.: "Strikingly different propulsive forces generated by different dynein deficient Chlamydomonas mutants in viscous media" Cell Motil. Cytoskel.31. 130-139 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yagi, T.: "A novel mode of hyper-oscillation in the paralyzed axoneme of a Chlamydomonas mutant lacking the central-pair microtubules" Cell Motil. Cytoskel.31. 207-214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogawa, K: "Interspecies conservation of outer arm dynein intermediae chain sequences defines two intermediate chain subclasses" Molec. Biol. Cell. 6. 685-696 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kamiya, R.: "Exploring the function of inner and outer dynein arms with Chlamydomonas mutants" Cell Motil. Cytoskel.32. 98-102 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura, S.: "Assembly and function of Chlamydomonas flagellar mastigonemes as probed with a monoclonal antibody" J.Cell Sci.109. 57-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shingyoji, C.: "Effect of beat frequency on the velocity of microtubule sliding in reactivated sea urchin sperm flagella under imposed head vibration" J.Exp. Biol.198. 645-653 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shingyoji, C.: "Cyclic bending movements induced locally by successive iontophoretic application of ATP to an elastasetreated flagellar axoneme" J.Cell Sci.108. 1359-1369 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shingyoji, C.: "Flagellar quiescence response in sea urchin sperm induced by electric stimulation" Cell Motil. Cytoskel.31. 59-65 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi, K.: "Mechanisms of flagellar motility probed with microtechniques" Cell Motil. Cytoskel.32. 110-113 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimizu, T.: "Comparison of the motilie and enzymatic properties of two microtubule minus-end motors, ncd and cytoplasmic dynein" Biochemostry. 34. 1575-1582 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura, K.: "Proteolysis of tubulin isotypes within Tetrahymena axonemes" Biol. Chem. Hoppe Seyler. 372. 729-732 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takaya, C.: "Comparison of axonemal proteins from two kinds of Tetrahymena" Comp. Biochem. Physiol.112B. 727-732 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada, Y.: "Effects of neurotransmitters on ciliary beating of sea urchin plutei" Physiol. Zool. 68. S48 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mohri, H.: "Outer arm dynein of sperm flagella and cilia in the animal kingdom" Memoires du Museum National D'Histoire Naturelle. 166. 15-22 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Oda, S.: "Sperm-activating proteins from unfertilized eggs of the Pacific herring, Clupea pallasii" Devel. Growth Differ.37. 257-261 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takai, H.: "Change in intracellular K^+ concentration caused by external osmolality change regulates sperm motility of marine and fresh water teleosts" J.Cell Sci.108. 1175-1181 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Togo, T.: "Existence of three mechanisms for blocking polyspermy in oocytes of the mussel Mytilus edulis" Biol. Bull.189. 330-339 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kinoshita, S.: "Regulatory role of nucleotides in axonemal function" Cell Motil. Cytoskel.32. 46-54 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Si, Y.: "Activation of mammalian sperm motility by regulation of microtubule sliding via cyclic adenosine 5'-monophosphate-dependent phosphrylation" Biol. Reprod.53. 1081-1087 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Si, Y.: "Extrusion of microtubule doublet outer dense fibers 5-6 associating with fibrous sheath sliding in mouse sperm flagella" J.Exp. Zool. 273. 355-362 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Omoto, C.K.: "Ability of paralyzed flagella mutants of Chlamydomonas to move" Cell Motil. Cytoskel.33. 88-94 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugase, Y.: "Cloning and characterization of the actin-encoding gene of Chlamydomonas reinhardtii" Gene. 168. 117-121 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada, S.: "Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer doublet microtubules" Cell Motil. Cytoskel.36. 68-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshimura, K.: "Conversion of beating mode in Chlamydomonas flagella induced by electric stimulation" Cell Motility and the Cytoskeleton.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamori, M: "Structure and function of the pore canals of the sea urchin madreporite" Phil. Trans. Roy. Soc. Lond. B.351. 659-676 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Noguchi, M.: "Effects of organic solvents on the coupling between ATP hydrolysis and sliding of microtubules in axonemes from Chlamydomonas flagella" J.Euk. Microbiol. 44. 127-133 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogawa, K.: "Is outer arm dynein intermediate chain 1 multifunctional?" Molec. Biol. Cell. 7. 1895-1907 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishijima, S.: "Calcium-dependent bidirectional power stroke on the dynein arms in sea urchin sperm" J.Cell Sci.109. 2833-2842 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogawa, K.: "A dynein motor superfamily" Cell Struct. Funct.21. 343-349 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imafuku, Y.: "Fluctuation in the microtubule sliding movement driven by kinesin in vitro" Biophysical Journal. 70. 878-886 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Edamatsu.M.: "Isolation and characterization of pos mutants defective in correct positioning of septum in Shizosaccharomyces pombe" Zoological Science. 13. 235-239 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Minamikawa, S.: "Acquisition, initiation and maintenance of sperm motility in the shark Trikias sehcha" Comp. Biochem. Physiol.113. 387-392 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inada, K.: "Identification of tubulins associated with the 950 kDa protease from salmon sperm" Biomedical Research. 17. 87-93 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Togo, T.: "Aminopeptidase-like protease released from oocytes suppresses acrosome reaction of sperm and established polyspermy block in oocytes of the mussel" Developmental Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wada, Y.: "Modification of ciliary beating in sea urchin larvae induced by neurotransmetters : beat-plane rotation and control of frequency fluctuation" Journal of Experimental Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Omoto,C.K.: "Ability of paralyzed flagella mutants of Chlamydomonas to move" Cell Motility and the Cytoskeleton. 33. 88-94 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sugase,Y.: "Cloning and characterization of the actin-encoding gene of Chlamydomonas reinhardtii" Gene. 168. 117-121 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takada,S.: "Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer-doublet microtubules" Cell Motility and the Cytoskeleton. 36. 68-75 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshimura,K.: "Conversion of beating mode in Chlamydomonas flagella induced by electric stimulation." Cell Motility and the Cytoskeleton. 36. 印刷中 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tamori,M.: "Structure and function of the pore canals of the sea urchin madreporite" Philosphical Transaction of.Royal.Society.London.B.351. 659-676 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Noguchi,M.: "Effects of organic solvents on the coupling between ATP hydrolysis and sliding of microtubules in axonemes from Chlamydomonas flagella" Journal of Eukaryotic Microbiology. 44. 127-133 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ogawa,K.: "Is outer arm dynein intermediate chain 1 multifunctional?" Molecular Biology of the Cell. 7. 1895-1907 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishijima,S.: "Calcium-dependent bidirectional power stroke on the dynein arms in sea urchin sperm" Journal of Cell Science. 109. 2833-2842 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ogawa,K.: "A dynein motor superfamily" Cell Structure and Function. 21. 343-349 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Imafuku,Y.: "Fluctuation in the microtubule sliding movement driven by kinesin in vitro" Biophysical Journal. 70. 878-886 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Edamatsu,M.: "Isolation and characterization of pos mutants defective in correct positioning of septum in Shizosaccharomyces pombe" Zoological Science. 13. 235-239 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Minamikawa,S.: "Acquisition,initiation and maintenance of sperm motility in the shark Trikias sehcha" Comparative Biochemistry and Physiology. 113. 387-392 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inaba,K.: "Identification of tubulins associated with the 950 kDa protease from salmon sperm" Biomedical Research. 17. 87-93 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Togo,T.: "Aminopeptidase-like protease released from oocytes suppresses acrosome reaction of sperm and established polyspermy block in oocytes of the mussel" Developmental Biology. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Wada,Y.: "Modification of ciliary beating in sea urchin larvae induced by neurotransmetters:beat-plane rotation and control of frequency fluctuation." Journal of Experimental Biology. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Minoura, I.: "Strikingly different propulsive forces generated by different dynein deficient chlamydomonas mutants in viscous media" Cell Motil. Cytoskeleton. 31. 130-139 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yagi, T.: "Novel mode of hyper-ocillation in the paralyzed axoneme of a Chlamdomonas mutant lacking the centralpair microtubules" Cell Motil. Cytoskeleton. 31. 207-214 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ogawa, K.: "Interspecies conservation of outer arm dynein intermediac chain sequences defines two intermediate chain subclasses" Molec. Biol. Cell. 6. 685-696 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kamiya, R.: "Exploring the function of inner and outer dynen arms with Chlamydomonas mutants" Cell Motil. Cytoskeleton. 32. 98-102 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakamura, S.: "Assembly and function of Chlamydomonas flagellar mastigonemes as probed with a monclonal antibody" J. Cell Sei.109. 57-62 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Omoto, C. K.: "Abillty of paralyzed flagella mutants of Chlamydomonas to move" Cell Motil. Cytoskeleton. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Sugase, Y.: "Cloning and characterization of the actin-encoding gene of Chlamydomonas reinhardtii" Gene. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Shigyoji, C.: "Effect of beat frequency on the velocity of microtubule sliding in reactivated sea urchin sperm flagella under imposed head vibration." J. Exp. Biol.198. 645-653 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shingyoji,C.: "Cyclic bending movements induced locally by successive iontophoretic application of ATP to an elastase-treated flagellar sxoneme." J. Cell Sci.108. 1359-1369 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shingyoji, C.: "Flagellar quiescence response in sea urchin sperm induced by electric stimulation" Cell Motil. Cytoskel.31. 59-65 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahashi, K.: "Mechanisms of flagellar motility probed with microtechniques" Cell Motil. Cytoskel. 32. 110-113 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimuzu, T.: "Comparison of the motilie and enzymatic properties of two microtubule minus-end motors, ncd and cytoplasmic dynein" Biochemistry. 34. 1575-1582 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakamura, K.: "Proteolysis of tubulin isotypes within Tetrahymena axonemes" Biol. Chem. Hoppe Seyler. 372. 729-732 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takaya, C.: "Comparison of axonemal proteins from two kinds of Tetrahymena" Comp. Biochem. Physiol. 112B. 727-732 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wada, Y.: "Effects of neurotransmitters on cillary beating of sea urchin plutei" Physiol. Zool.68. S48 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mohri, H.: "Outer arm dynein of sperm flagella and cilla in the animal kingdom" Memoires du Museum National D'Histoire Naturelle. 166. 15-22 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Minamikaw, S.: "Acquistion, initiation and maintenance of sperm motility in the shark, Triakis scyllia" Comp. Biochem. Physiol.(印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Oda. S.: "Sperm-activating proteins from unfertilized eggs of the Pacific herring, Clupea Pallasii" Develop. Growth & Differ.37. 257-261 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takai, H.: "Change in intracellular K^+ concentration caused by external osmolality chage regulates sperm motility of marine and freshwater teleosts" J. Cell. Sci.108. 1175-1181 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Togo, T.: "Existence of theree mechanisms for blocking polyspermy in oocytes of the mussel Mytilus edulis" Biol. Bull.189. 330-339 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Inaba, K.: "Identification of tubulins associated with the 950 kDa protease from salmon sperm" Biomed. Res.(印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Kinoshita, S.: "Regulatory role of nucleotides in axonemal function" Cell Motil. Cytoskeleton. 32. 46-54 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Si, Y.: "Activation of mammalian sperm motility by regulation of microtubule sliding via cyclic adenosine 5'- monophosphate-dependent phosphrylation" Biol. Reprod.53. 1081-1087 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Si, Y.: "Extrusion of microtubule doublet outer dense fibers 5-6 associating with fibrous sheath sliding in mouse sperm flagella" J. Exp. Zool.273. 355-362 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yagi,T.: "Nanometer-scale vibration in mutant axonemes of Chlamydomonas." Cell Motil.Cytoskeleton. 29,. 177-185 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takada,S.: "Functional reconstitution of Chlamydomonas outer dynein arms from α-β and γ subunits:requirement of a third factor." J.Cell Biol.126. 37-745 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Muto,E.: "Immunological detection of actin in the 14S ciliary dynein of Tetrahymena." FFBS Lett.343. 173-176 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Yoshida: "Calcium and cyclic AMP mediate sperm activation,but Ca alone contributes sperm chemotaxis in the ascidian,Clons savignyl" Develop,Growth & Differ.36. 589-595 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Z.X.Jin: "Monoclonal antibodles against the protein complex that contains the flagellar movement-initiating phosphoprotein of Oncorhynchus keta" J.Biochem. 115. 885-890 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Morisawa: "Cell signalling mechanisms for sperm motility" Zooi.Sci.11. 647-662 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Z.X.Jin: "Isolation and properties of a protein complex that contains flagellar movement-initiating phosphoprotein from testes of a white salmon" J.Biochem.115. 552-556 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimizu,T.: "Characterization of the motile and enzymatic properties of two minus-end directed microtubule motors,cytoplasmic dynein and ncd." Biochemistry. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mimori,Y.: "Extrusion of rotating microtubules on the dynein-track from a microtubule-dynein γ-complex" Cell Motil.Cytoskel.30. 17-25 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mimori,Y.: "ATP-induced sliding of microtubules on tracks of 22S dynein molecules aligned with the same polarity" Cell Motil.Cytoskel.27. 180-191 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Si: "The extrusion of 5-6 outer doublet microtubules associating with fibrous sheath sliding in mouse sperm flagella" J.Exp.Zool.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] F.Aoki: "Analysis of flagellar bending in hyperactivated hamster and mouse spermatozoa" J.Reprod.& Fertil.101. 397-403 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shingyoji,C.: "Effect of beat frequency on the velocity of microtubule sliding in reactivated sea urchin sperm flagella under imposed head vibration." J.Exp.Biol.198(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shingyoji,C.: "Cyclic bending movements induced locally by successive iontophoretic application of ATP to an elastase-treated flagellar axoneme." J.Cell Sci.108(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shingyoji,C.: "Flagellar quiescence response in sea urchin sperm induced by electric stimulation." Cell Motil.Cytoskel.(in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-03-31   Modified: 2016-04-21  

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