| Project/Area Number |
06304013
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 総合 |
|---|
| Research Field |
園芸・造園学
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
YAMAKI Shohei Nagoya University, Agricultural Sciences, Professor, 農学部, 教授 (70210341)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NITO Nobumasa Saga University, Agriculture, Professor, 農学部, 教授 (80015809)
SUGIURA Akira Kyoto University, Agriculture, Professor, 農学部, 教授 (00026379)
MII Masahiro Chiba University, Horticulture, Professor, 園芸学部, 教授 (30093074)
MINAMIKAWA Takao Tokyo Metoro.University, Science, Professor, 理学部, 教授 (30087001)
AKIHAMA Tomoya Meiji University, Agriculture, Professor, 農学部, 教授 (90167841)
|
|---|
| Project Period (FY) |
1994 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
|
| Budget Amount *help |
¥22,300,000 (Direct Cost: ¥22,300,000)
Fiscal Year 1996: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1995: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1994: ¥12,200,000 (Direct Cost: ¥12,200,000)
|
|---|
| Keywords | ACCsynthase cDNA / Cysteine protease gene / Sucrose phosphate synthase cDNA / Sorbitol dehydrogenase cDNA / S6PDH transgenic apple / Transgenic grape / Transgenic citrus / Transgenic persimmon / ACC合成酵素遺伝子 / ACC酸化酵素遺伝子 / S6PDH遺伝子 / SPS遺伝子 / リンゴの形質転換体 / ソルビトール6リン酸脱水素酵素遺伝子 / PSP遺伝子 / カキの形質転換 / キウイフルーツの形質転換 / カンキツの形質転換 / リンゴの形質転換 / ACC酸化酵素cDNA / ソルビトール-6-リン酸脱水素酵素cDNA / ソルビトール脱水素酵素 / ペプチダーゼcDNA / β-ガラクトシダーゼ / キウィフルーツの形質転換 |
|---|
| Research Abstract |
This project was conducted by 2 groups. The first group tried the purification and cloning of some enzymes which concern closely the improvement of fruit quality. The base sequence coding cDNA of sorbitol dehydrogenase which converts sorbitol of translocating sugar in Rosaceae fruit to fructose was determined in apple fruit. In tomato fruit, cDNAs of 6 isozymes of ACCsynthase were isolated and the expressions were investigated using the specific antibody for each isozyme. From melon fruit, the cDNA encoding ACCsynthase and ACCoxidase were isolated, the base sequences were determined and the gene expression were investigated with fruit development. The base sequence of cDNA encoding sucrose phosphate synthase, sucrose synthase and acid invertase concverning the biosynthesis and degradation of sucrose of translocating sugar in Citrus were determined. The gene encoding cysteine protease which functions the maturation and senscence was isolated from French bean seed and Vigna mungo seedling, and the structure of promotor and the gene expression were investigated. alpha-Arabinofurano sidase concerning the softening of Japanese pear fruit was purified.
The second group tried to establish the plant-regeneration system and gene transfer method to for the transgenic fruit tree. In apples, the plant-regeneration from the callus of apple cultivar and the gene transfer via Agrobacterium became possible, and we succeeded the transfer of S6PDH chimeric gene. In persimmon, the plant was regenerated from callus and protoplast, and the recombinant of marker gene was formed through Agrobacterium infection. Further, in grape and citurs belonging sub-family of Rutaceae, we succeeded to get the embryogenic callus and to form the recombinant of GUS gene by co-culturing the callus with Agrobacterium. As described above, we could collect some genes concerning closely the fruit quality, and establish the plant-regeneration method and the gene trans fer method in main fruits crops in Japan.
|
