| Project/Area Number |
06304022
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | the University of Tokyo |
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Principal Investigator |
HASEGAWA Atsuhiko UNIV.of TOKYO,PROFESSOR, 農学部, 教授 (90011923)
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| Co-Investigator(Kenkyū-buntansha) |
KUNIEDA Tetsuo UNIV.of OKAYAMA,ASSOCIATE PROFESSOR, 農学部, 助教授 (80178011)
WATARI Toshihiro UNIV.of TOKYO,RESEARCH ASSOCIATE, 農学部, 助手 (50220950)
HIRAI Katsuya UNIV.of GIFU,PROFESSOR, 農学部, 教授 (30021702)
MATSUMOTO Toshitsugu UNIV.of TOKYO,ASSOCIATE PROFESSOR, 農学部, 助教授 (00173922)
TSUJIMOTO Hajime UNIV.of TOKYO,ASSOCIATE PROFESSOR, 農学部, 助教授 (60163804)
高橋 清志 酪農学園大学, 酪農学部, 教授 (90048108)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1995: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | ANIMAL / DNA DIAGNOSIS / HEREDITARY DISEASE / INFECTIONS DISEASE / TUMOR / VIRUS / RICKETTSIA / 個体識別 / 臨床診断 / ネコ白血病ウイルス / ネコ免疫不全ウイルス / MHC / クラミジア / 白血球粘着分子異常症 / 遺伝子再構成 |
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| Research Abstract |
To establish various DNA diagnosis systems in the veterinary medicine, we carried out diagnostic researches on hereditary diseases, infectious diseases, tumors and animal MHC_s.
Hereditary diseases : In bovine leukocyte adhesion deficiency, 128Asp of CD 18 molecule was found to be substituted by Gly. In bovine blood coagulation factor XIII deficiency, 83Phe of FXIIIA gene was shown to be substituted by Ser. PCR diagnosis systems were developed for both of these hereditary diseases.
Infectious diseases : PCR detection systems could be developed for Chlamydia psittaci and Coxiella burnetii in various animal species. In feline immunodeficiency virus infectoin, in addition to the establishment of PCR detection sysytem, identification system for FIV subtypes could be developed. PCR detection system for bovine immunodeficiency virus was also established.
Tumors : DNA diagnosis system to identify lineage and clonarity of the lymphoid tumor cells was developed by using Southern blot analysis with T-cell receptor and immunoglobulin gene probes. PCR-SSCP analysis of canine p53 gene demonstrated the presence of p53 mutaions in a variety of tumors including lymphoma, leukemia, osteosarcoma and colon cancer. Examination of oncogenes and retroviral common integration sites revealed viral-induced abnormalities in 5 of 11 naturally occurring lymphomas and a correlation of c-myc abnormality with abnomality of other oncogenes.
Animal MHC_s : Two kinds of MHC DRB clones were isolated from a canine B lymphoblastois cell line, and MHC typing became available by using these sequences.
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