| Project/Area Number |
06304029
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
HARADA Shoji Institute of Community Medicine, University of Tsukuba Associate Professor, 社会医学系, 助教授 (60086618)
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| Co-Investigator(Kenkyū-buntansha) |
KUROSAKI Kunihiko Dept.Legal Med., Chiba, Univ.Lecturer, 医学部, 講師 (60240701)
SUZUKI Koichi Dept.Legal Med., Ohita Med.Coll., Professor, 医学部, 教授 (60171211)
YAMANOUCHI Haruo Dept.Legal Med., Niigata, Univ.Studies, 医学部, 教授 (30134919)
TAMAKI Yoshihiro Dept.Legal Med., Ohita Med.Coll., Professor, 医学部, 教授 (20028377)
KATSUMATA Yoshinao Dept.Legal Med., Nagoya, Univ.Professor, 医学部, 教授 (30109326)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1995: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | Human specific DNA / Y-Chromosome specific DNA / Microsatellite DNA / Minisatellite DNA / MVR-PCR / Tandem repeats / Heterozeigosity / DNA Profiling / Y-chromosome specific DNA / Tandem Repeats |
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| Research Abstract |
It provides important information for forensic DNA profiling to evaluate technology relating DNA analysis, forensic application of DNA polymorphic loci such as STR and RFLP,data base and biostatistical analysis, legal and ethical aspects of DNA test. In these aspects, we made intensive study concerning diagnosis of human specific region, sex typing and individual indentification from forensic materials. Also symposium and meetings were held in the period of research project.
1) For determination of genetic uniqueness of individual species, human specific DNA region (HSS) were analyzed using PCR method for forensic materials (saliva, sperms, blood stains, hairs, tissues, bones and tooth). It was confirmed that HSS region was detected only in human genomes involving Mongoloid, Caucasoid and Negroid, and was absent in non-human primates.
2) DXZ 4 and SPY genes were examined to ascertain the feasibility of a sex-typing procedure using PCR.The DXZ 4 amplification could detect the 80bp fragment from female DNA.The SPY probe identified a male-specific sequence on chromosome Y.
3) Individual identification from forensic blood samples were performed for MCT118, ACTBP2, FGA,D8S320, D11S488, TH01 and D16S537 loci using PCR and relating technology. Allelic frequencies of these loci were obtained from 200-300 Japanese population. In addition, microsatellite variant repeat (MVR) mapping using PCR at D1S8 (MS32) was applied to a practical cases.
4) DNA sequence data for DNA profiling of forensic materials using STR systems are necessary and quality assurance guidances must be established in Japan. In addition, new technology for DNA sequencing requires to be introduced in forensic field.
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