| Project/Area Number |
06304035
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
General surgery
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
DOHI Kiyohiko Hiroshima University School of Medicine, Professor, 医学部, 教授 (90034024)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMACHI Keizo Kyushu University, Faculty of Medcine, Professor, 医学部, 教授 (00038762)
MONDEN Morito Osaka University Medical School, Professor, 医学部, 教授 (00127309)
TAKAGI Hiroshi Nagoya University School of Medicine Professor, 医学部, 教授 (70154755)
ISONO Kaichi Chiba University, School of Medicine, Professor, 医学部, 教授 (70009489)
FUKAO Katashi University of Tsukuba, Institute of Clinical Medicine, Professor, 臨床医学系, 教授 (50091921)
内田 久則 東京大学, 医科学研究所, 教授 (30050420)
里見 進 東北大学, 医学部, 教授 (00154120)
内野 純一 北海道大学, 医学部, 教授 (40000989)
森 昌造 東北大学, 医学部, 教授 (70004877)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥28,000,000 (Direct Cost: ¥28,000,000)
Fiscal Year 1996: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1995: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1994: ¥14,000,000 (Direct Cost: ¥14,000,000)
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| Keywords | Liver transplantation / Organ preservation / Non-heart-beating donor / Ischemia / reperfusion injury / Network for organ transplantation / Living-related liver transplantation / Immuno-regulation / Xenotransplantation / 虚血再灌流障害 / 脳死ドナー / 再灌流障害 / 免疫寛容 / 抗接着分子抗体 |
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| Research Abstract |
1.Organ procurement and organ preservation : In the study regarding the relevance of donor nutritional state to graft liver function, it has been demonstrates that poor nutrition of donor caused the endotherial damage of liver sinusoids and results in the impediment to ability of mitochondrial ATP synthesis rates (Satomi and Tanaka). In the study regarding the organ harvesting, the effect of donor hypothermic therapy on graft function and the proper flow rate of initial flushing of graft organs were investigated (Taira). As an useful graft liver viability assays, hepatic protein synthesis rate assay using the graft tissues obtained by needle biopsy and graft effuent assay were developed (Isono and Sugimachi). For taking measures against ischemic damage of graft liver from non-heart beating donor, the novel methods such as the core cooling technique were investigated (Uchino, Satomi and Fukao).
2.Indication of liver transplantation : The objective criterions of indication and emergency l
… More
evel for liver transplantation were described for trial (0ta). For the development of proper registration program for liver transplantation recipients, the detailed follow-up-study of the registered recipientswas done (Sugimachi). A predictive equation for non-A,non-B acute hepatitis was developed for the criterion of indication for liver transplantation (Tanigawa).
3.Postoperative management for liver transplantation : The effect of graft liver size-matching on the postoperative graft function and the problem concerning FK506 immunosuppressive therapy in clinical living-related liver transplantation were researched (Yamaoka). As a novel development of immunosuppressive therapy, the drug-delivery modification for the conventional immunosuppressants (intraportal and intra-hepatic-arterial administration of FK506), the induction of immuno-hyporeactivity utilizing donor-antigen infusion (MMC-treated donor splenocyts infusion), the immuno-regulation by use of gene-therapy (CTLA-Ig gene transduction using adenovirus vector), the use of a novel immunosuppressant (Bactobolamine) were researched (Dohi, Monden, Takagi and Tomikawa).
4.Study of xenotransplantation : In order to develop the immuno-regulation method in concordant xenotransplantation, the effects of donor bone marrow utilization and the modification of donor antigen with MMC-treatment were investigated (Dohi and Monden). For the purpose of preventing hyperacute rejection in discordant xenotransplantation, human complement regulating genes were successfully transduced in pigs by use of retrovirus vector (Takagi). Less
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