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The immunologically and molecular biologically investigation for diagnostic methods of periodontal disease activity

Research Project

Project/Area Number 06304041
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

OKADA Hiroshi  Osaka Univ.Fac.of Dentistry, Dept.of Periodontol.and Endodontol., Professor., 歯学部, 教授 (40038865)

Co-Investigator(Kenkyū-buntansha) MAITA Eikichi  Tohoku Univ.School of Dentistry, Dept.of Endodontol.and Periodontol., Associate, 歯学部, 助教授 (80108547)
KATO Ihachi  Nagasaki Univ.School of Dentistry, Dept.of Periodontol., Professor., 歯学部, 教授 (30005087)
MAEDA Katumasa  Kyusyu Univ.Fac.of Dentistry, Dept.of Periodontol.and Endodontol., Professor., 歯学部, 教授 (00117243)
MURAYAMA Youji  Okayama Univ.Dental School, Dept.of Periodontol.and Endodontol., Professor., 歯学部, 教授 (50029972)
MURAI Seidai  Nihon Univ.Fac.of Dentistry, Dept.of Periodontol., Professor., 歯学部, 教授 (50059185)
和泉 雄一  九州大学, 歯学部, 教授 (60159803)
岡本 莫  広島大学, 歯学部, 教授 (50028742)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥16,400,000 (Direct Cost: ¥16,400,000)
Fiscal Year 1995: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1994: ¥10,100,000 (Direct Cost: ¥10,100,000)
KeywordsPeriodontal disease activity / Inflammatory cytokine / Alkaline phosphatase / Periodontopathic bacteria / Polymerase chain reaction / Neutrophil lysosomal proteinase / PH in periodontal pockets / Secretory leukocyte protease inhibitor / 歯周病 / 疾病活動 / 内毒素 / 蛋白分解酵素-インヒビター複合体 / DNAプローブ / リソゾーム性カリンプロテアーゼ / 歯肉溝浸出液 / 歯周ポケット
Research Abstract

This study was designed to immunologically and molecular biologically evaluate the diseased conditions of periodontitis and develop procedures to diagnose active lesions and susceptible patients. The techniques utilizing DNA probes and polymerase chain reaction were introduced to sensitively detect and identify periodontopathic bacteria in subgingival plaque. It was shown that P.gingivalis (Pg) was frequently detected in active diseased lesions. Additionally, T.denticola (Td) was always found in the unsuccessfully treated periodontal lesions but not in the successfully treated lesions, suggesting that Td would be a diagnostic indicator of the diseased conditions in the lesions after periodontal treatments. It was also suggested that Vgamma9/Vdelta2 T cells may play an important role in prevention of bacterial infection or tissue destruction. Futhermore, immune response against heat shock protein (HSP) 60 may react with both periodontitis-associated microorganisms and host cells because … More HSP 60 specific antibodies of periodontitis patients crossreact to proteins isolated from gingival fibroblast. In addition, it was also indicated that the levels of serum actibodies specific to Pg could be used as a risk indicator for the progression of periodontal diseases, because the susceptible patients to periodontal diseases exhibited significantly higher anti-Pg antibody levels than the resistant patients. Furthermore, the high levels of polyamines, alkaline phosphatase, platelet-activating factor activities, neutrophil lysosomal proteinase (medullasin and cathepsin G), secretory leukocyte protease inhibitor (SLPI), inflammatory cytokines (IL-1, IL-6, Il-8) and prostaglandin E_2 were frequently observed in gingival crevicular fluid. Also pH in periodontal pockets was significantly high in the sites with deep probing depth. Parameters showing above seem to be useful to diagnose active periodontal diseases. In conclusion, it was suggested that the combining host immune responses, inflammatory cytokines and mediators in local diseased sites examined in this study may enable us to diagnose periodontal dosease activity. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] H. Okada, et al.: "Diagnostic strategies of periodontitis based on the molecular mechanisms of periodontal tissue destruction" Oral Diseases. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Kunimatsu, et al.: "Identification and possible function of cathepsin G in gingival crevicular fluid from chronic adult periodontitis patients and from experimental gingivitis subjects" Journal of Periodontal Research. 30. 51-57 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] O. Fujise, et al.: "Colorimetric microtiter plate based assay for detection and quantification of amplified Actinobacillus actinomycetemcomitans DNA" Oral Microbiology and Immunology. 10. 372-377 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 村井 正大,他: "唾液、GCF中のALP活性について" 日大歯学(掲載予定). 70. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 結城 哲二,他: "16SrRNA遺伝子増幅による歯周病原性細菌の迅速な同定法の検討" (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大竹 徹,他: "DNAプローブを用いた細菌検査の予後判定への試み" 日本歯周病学会誌. 38(発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Okada et al: "Diagnostic strategies of periodontitis based on the molecular mechanisms of periodontal tissue destruction" Oral Diseases. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kunimatsu et al: "Identification and possible function of cathepsin G in gingival crevicular fluid from chronic adult periodontitis patients and from experimental gingivititis subjects" Journao of Periodontal Research. 30. 51-57 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] O.Fujise et al: "Colorimetric microtiter plate based assay for detection and quantification of amplified Actinobacillus actinocycetemcomitans DNA" Oral Microbiology and Immunology. 10. 372-377 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Okada et al: "Diagnostic strategies of periodontitis based on the molecular mechanisms of periodontal tissue destruction" Oral Diseases(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Kunimatsu et al: "Identification and possible function of cathepsin G in gingival crevicular fluid from chronic adult periodontitis patients and from experimental gingivitis subjects" Journal of Periodontal Reseach. 30. 51-57 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] O.Fujise et al: "Colorimetric microtiter plate based assay for detection and quantification of amplified Actinobacillus actinomycetemcomitans DNA" Oral Microbiology and Immunology. 10. 372-377 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 村井 正大 ら: "唾液、GCF中のALP活性について" 日本歯学. 70(掲載予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 結城 哲二 ら: "16SrRNA遺伝子増幅による歯周病原性細菌の迅速な同定法の検討" (発表予定).

    • Related Report
      1995 Annual Research Report
  • [Publications] 大竹 徹 ら: "DNAプローブを用いた細菌検査の予後判定への試み" 日本歯周病学会誌. 38(発表予定).

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2018-02-02  

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