| Project/Area Number |
06304044
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
NAKANISHI Mamoru Nagoya City Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (90090472)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAJIMA Kouichiro Kyoto Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (30025689)
HASHIDA Mitsuru Kyoto Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (20135594)
KIRINO Yutaka Tokyo Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (10012668)
IMAI Kazuhiro Tokyo Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (50012620)
KAMO Naoki Hokkaido Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (10001976)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1995: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | Durg absorption / Confocal Microscopy / Scanning Probe Microscopy / Investigation of New Drug / Absorption and Transportion / Optical microscopic technique / 顕微光学技術 / 経皮吸収 / 初回通回効果 / 高分子薬物 |
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| Research Abstract |
A renaissance and a revolution in light microscopy are now under way. Several new technologies, used in conjunction with the light microscope, have yeilded a new way of seeing and measuring drugs in living cells. One major contribution to the new methodology is the use of computers for the digital processing and analysis of images. The second vital innovation in the new microscopy is the development of multicolor fluorescent dye molecules which represent a modern version ofselective stains relies on by earlier generations of microscopists. In this projects we have tried to study bioimaging for drug a bsorption in living cells by new light microscopes. We obtained various new findings for this subject. They were the studies of the surface expression of CD63 antigen in P815 mastocytoma cells by transfected IgE receptors, the conducance change in phospholipid bilayr membrace by an electroneutral ionophore (monensin), the application and prospects for physiologically based pharmacokinetic models involving pharmaceutical agents, the galactosylated proteins recognized by the liver according to the surface density of galactose, and cloning and characterization of rat H^+/peptidecotransporter mediating absorption of beta-lactam antibiotics in the intestine and kidney. Thus, the present Grant-in-Aid for Co-operative Research was able to obtain an extreemly high gool in the field of drug absorption in living cells.
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