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Studies on Functions of bioactive phospholipids

Research Project

Project/Area Number 06304050
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field Structural biochemistry
Research InstitutionGIFU UNIVERSITY

Principal Investigator

NOZAWA Yoshinori  GIFU UNIVERSITY,FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (10021362)

Co-Investigator(Kenkyū-buntansha) SIMIZU Takao  TOKYO UNIVERSITY,FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (80127092)
IKEZAWA Hiroo  NAGOYA CITY UNIVERSITY,FACULTY OF PHARMACEUTICAL SCIENCES,PROFESSOR, 薬学部, 教授 (40080163)
INOUE Keizo  TOKYO UNIVERSITY,FACULTY OF PHARMACEUTICAL SCIENCES,PROFESSOR, 薬学部, 教授 (30072937)
KANOH Hideo  SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE,PROFESSOR, 教授 (70045475)
NISHIJIMA Masahiro  NATIONAL INSTITUTE OF HEALTH, THE HEAD, 細胞化学部, 部長 (60072956)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥27,600,000 (Direct Cost: ¥27,600,000)
Fiscal Year 1996: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1995: ¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 1994: ¥11,400,000 (Direct Cost: ¥11,400,000)
KeywordsPhospholipases (A2, C,D) / Bioactive phospholipid / PAF-receptor / PAF-acetylhydrolase / GPI-anchor / PA phosphohydrolase / PIP2-binding protein / ホスホリパーゼA2,C,D / 情報変換素子 / 情報変換ホスホリパーゼ / スフィンゴ脂質 / 膜リン脂質
Research Abstract

(1) PLD was synergistically activated by PKCalpha and RhoA.Rat PLD2 cDNA was cloned and mRNA levels of rPLD1a, 1b and 2 were observed to change during proliferation and differentiation (Nozawa). (2) cDNA of PAF-acetylhydrolase of guinea-pig blood plasma was isolated and the primary structure of the enzyme was determined (Nojima). (3) The CD14 was found to be the endotoxin (LPS) receptor. The signaling through LPS receptor requires PC breakdown by PLD (Nishijima). (4) The PLCdelta4 cDNA was cloned and its mRNA was expressed in the regenerating rat liver cell nuclei. A downstream molecules of Ash/Grb2 was PIP2 phosphatase (Takenawa). (5) DGKdelta and PAP2a, b were cloned. PAP2a was identified to be a Wunen homologue and PAP2b was Dri gene (Kanoh). (6) The image analysis with fluorescence microscope has shown that PS and some cytosolic factor(s) were required for the PS transfer to Golge bodies (Ohki). (7) PAF-acetylhydrolase was found to be composed of 3 subunits (alpha1, alpha2=catalytic subunits). beta subunit is the product of LIS-1 gene of Miller-Dieker syndrome (Inoue). (8) The amino acid sequences of the N-terminal and internal part of amino peptidase N were determined. GPI-anchored protein(s) was found in Sulfolobus (Ikezawa). (9) Expression of the intestinal phospholipase B-cDNA revealed that this enzyme was a novel serine esterase (Okamoto). (10) Sphingosine showed structural specific inhibition of DNA polymerase. PLA_2 purified from the chromatin fraction of rat ascitic hapatoma was 17kDa PLA_2II which was specifically localyzed (Koizumi). (11) It was demonstrated that 2 splicing variants of PAF-receptor genes were present and that leucocyte regulated by distinct promotors. Physiological functions were examined by using knock-out mice (Shimizu).

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (91 results)

All Other

All Publications (91 results)

  • [Publications] Y. Banno: "Thrombin-mediated phosphoinositide hydrolysis in CHO cells overexpressing phospholipase C-δ1" J. Biol. Chem.269. 15846-15852 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Ohashi: "Peripheral blood cells are predominantly chimeric of affected and normal cells in patients with paroxysmal nocturnal hemoglobinuria" Blood. 83. 853-859 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Hattori: "Miller-Dieker lissencephaly gene encodes a subunit of brain platelet-activating factor acetylhydrolase" Nature. 370. 216-218 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Kai: "Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells" J. Biol. Chem.269. 18492-18498 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Asano: "Purification and characterization of nuclear phospholipase C specific for phosphoinositides" J. Biol. Chem.269. 12360-12366 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Tanaka: "C3G, a guanine nucleotide-releasing protein expressed ubiquitously, binds to the src homology 3 domains of crk and grb2/ash proteins" Proc. Natl. Acad. Sci.91. 3443-3447 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Okutani: "Grb2/Ash binds directly to tyrosine kinase 1068 and 1086 and indirectly to tyrosine 1148 of activated human epidermal growth factor receptors in intact vells" J. Biol. Chem.269. 31310-31314 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Bito: "Functional coupling of a rat hippocampal somatostatin receptor to inhibition of adenylate cyclase, activation of arachidonate release and MAP kinase" J. Biol. Chem.269. 12727-12730 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Gotoda: "Adult-onset spinocerebellar dysfunction caused by a mutation in the gene for the α-tocopherol-transfer protein" New Eng. J. Med.333. 1313-1318 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J. C. Hay: "ATP-dependent inositide phosphorylation required for Ca^<2+>-activated secretion" Nature. 374. 173-177 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Fukasawa: "Chinese hamster ovary cells expressing a novel type of acetylated low density lipoprotein receptor" J. Biol. Chem.270. 1921-1927 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] I. Wada: "Chaperon function of calreticulin when expressed in the endoplasmic reticulum as the membrane-anchored and soluble forms" J. Biol. Chem.270. 20298-20304 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Ouahchi: "Ataxia with isolated vitamin E deficiency is caused by mutations in the α-tocopherol transfer protein" Nature Genet.9. 141-145 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Hanada: "Both sphingolipids and cholesterol participate in the detergent insolubilityof alkaline phosphatase a glycosylphosphatidylinositol-anchored protein, in mammalian membranes" J. Biol. Chem.270. 6254-6260 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Banno: "Endogenous cleavage of phospholipase β3 agonist-induced calpain activation in human platelets" J. Biol. Chem.270. 4318-4323 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Mutoh: "Tissus-specific response of the human platelet-activating factor receptor gene to retinoic acid and thyroid hormone by alternative promoter usage" Proc. Natl. Acad. Sci.93. 774-779 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Ohguchi: "Regulation of membrane phospholipase D by protein kinase C in HL60 cells. Synergistic action of small GTP-binding protein RhoA" J. Biol. Chem. 271. 4366-4373 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Yokomizo: "cDNA cloning, expression, and mutagenesis study of leukotriene B4 12-hydroxy-dehydrogenase" J. Biol. Chem.271. 2844-2850 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Kai: "Identification and cDNA cloning of 35-kDa phosphatidic acid phosphatase (type 2) bound to plasma membranes" J. Biol. Chem.271. 18931-18938 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Moriya: "PDGF activates protein kinase C ε through redundant and independent signaling pathway involving phospholipase C γ or phosphatidylinositol 3-kinase" Proc. Natl. Acad. Sci.93. 151-155 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Tasaka: "Targeted mutagenesis of acyl-lipid desaturases in Synechocystis : Evidencefor the important roles of poly-unsaturated membrane lipids in growth, respiration and photosynthesis" EMBO J.15. 6416-6425 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Emoto: "Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis" Proc. Natl. Acad. Sci.93. 12867-12872 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Miki: "A novel actin-depolymerizing protein N-WASP regulates the cortical cytoskeletal rearrangement in a PIP2 dependent manner at the downstream of tyrosine kinase" EMBO J.15. 5326-5335 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y-S. Ho: "Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer" Nature. 385. 89-93 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Higashida: "Progress in Brain Reserch" (ed. Kruger, L.). Elsevier Science Publisher B. V., 15 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Nakashima: "Handbook of Experimental Pharmacology ″Platelets and their factors″" Platelet phospholipase C, D (Bruchhausen, V. and Walter, U., eds.) Springer-Verlag, 15 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Takenawa: "Advance in Pharmacol vol. 36 Intracellular Signal Transduction" Signal transductions of SH2/SH3 : Ash/Grb2 downstream signals (eds. Narin and Hidaka), 14 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Nakashima: "Advances in Molecular and Cell Biology Series Vol. 17 The Platelet" Role of signal-transducing phospholipase A2, C and D in platelet activation (Lapetina EG, ed.). JAI Press Inc, 21 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Kanoh: "Guidebook on calcium binding proteins" Diacylglycerol kinase (ed. by Celio, M. R.) Oxford University Press (in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Banno: "Thrombin-mediated phosphoinositide hydrolysis in CHO cells overexpressing phospholipase C-delta1" J.Biol.Chem. 269. 15846-15852 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Ohashi: "Peripheral blood cells are predominantly chimeric of affected and normal cells in patients with paroxysmal nocturnal hemoglobinuria" Blood. 83. 853-859 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Hattori: "Miller-Dieker lissencephaly gene encodes a subunit of brain platelet-activating factor acetylhydrolase" Nature. 370. 216-218 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kai: "Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells" J.Biol.Chem.269. 18492-18498 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Asano: "Purification and characterization of nuclear phospholipase C specific for phospho-inositides." J.Biol.Chem.269. 12360-12366 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Tanaka: "C3G,a guanine nucleotide-releasing protein expressed ubiquitously, binds to the src homology 3 domains of crk and grb2/ash proteins" Proc.Natl.Acad.Sci.91. 3443-3447 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Okutani: "Grb2/Ash binds directly to tyrosine kinase 1068 and 1086 and indirectly to tyrosine 1148 of activated human epidermal growth factor receptors in intact vells." J.Biol.Chem.269. 31310-31314 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Bito: "Functional coupling of a rat hippocampal somatostatin receptor to inhibition of adenylate cyclase, activition of arachidonate release and mitogen-activated (MAP) kinase" J.Biol.Chem.269. 12727-12730 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Gotoda: "Adult-onset spinocerebellar dysfunction caused by a mutation in the gene for the alpha-tocopherol-transfer protein" New Eng.J.Med.333. 1313-1318 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.C.Hay: "ATP-dependent inositide phosphorylation required for Ca^<2+>-activated secretion" Nature. 374. 173-177 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Fukasawa: "Chinese hamster ovary cells expressing a novel type of acetylated low density lipoprotein receptor" J.Biol.Chem.270. 1921-1927 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] I.Wada: "Chaperon function of calreticulin when expressed in the endoplasmic reticulum as the membrane-anchored and soluble forms" J.Biol.Chem.270. 20298-20304 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ouahchi: "Ataxia with isolated vitamin E deficiency is caused by mutations in the alpha-tocopherol transfer protein" Nature Genet.9. 141-145 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Hanada: "Both sphingolipids and cholesterol participate in the detergent insolubility of alkaline phosphatase a glycosylphosphatidylinositol-anchored protein, in mammalian membranes." J.Biol.Chem.270. 6254-6260 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Banno: "Endogenous cleavage of phospholipase beta3 by agonist-induced calpain activation in human platelets" J.Biol.Chem.270. 4318-4323 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Mutoh: "Tissue-specific response of the human platelet-activating factor receptor gene to retinoic acid and thyroid hormone by alternative promoter usage" Proc.Natl.Acad.Sci.93. 774-779 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ohguchi: "Regulation of membrane phospholipase D by protein kinase C in HL60 cells. Synergistic action of small GTP-binding protein RhoA" J.Biol.Chem.271. 4366-4373 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Yokomizo: "cDNA cloning, expression, and mutagenesis study of leukotriene B4 12-hydroxy-dehydrogenase" J.Biol.Chem.271. 2844-2850 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kai: "Identification and cDNA cloning of 35-kDa phosphatidic acid phosphatase (type 2) bound to plasma membranes." J.Biol.Chem.271. 18931-18938 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Moriya: "PDGF activates protein kinase Cepsilon through redundant and independent signaling pathway involving phospholipase Cgamma or phosphatidylinositol 3-kinase" Proc.Natl.Acad.Sci.93. 151-155 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Tasaka: "Targeted mutagenesis of acyl-lipid desaturases in Synechocystis : Evidence for the important roles of polyunsatureted membrane lipids in growth, respiration and photosynthesis" EMBO J.15. 6416-6425 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Emoto: "Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis" Proc.Natl.Acad.Sci.93. 12867-12872 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Miki: "A novel actin-depolymerizing protein N-WASP regulates the cortical cytoskeletal rearrangement in a PIP_2 dependent manner at the downstream of tyrosine kinase" EMBO J.15. 5326-5335 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y-S.Ho: "Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer" Nature. 385. 89-93 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Higashida (ed.Kruger, L.): Elsevier Science Publisher B.V.Progress in Brain Research, 15 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Nakashima: Platelet phospholipase C, D (Bruchhausen, V.and Walter, U., eds.) Springer-Verlag. Handbook of Experimental Pharmacology Platelets and factors, 15 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Takenawa: Signal transductions of SH2/SH3 : Ash/Grb2 downstream signals (eds. Naarin and Hidaka). Advance in Pharmacol vol.36, Intracellular Signal Transduction, 14 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Nakashima: Role of signal-transducing phospholipase A2, C,D in platelet activation (Lapetina E.G., ed.) JAI Press Inc. Advances in Molecular and Cell Biology Series Vol.17 The Platelet, 21 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanoh: Diacylglucerol kinase (ed.by Celio, M.R.) Oxford University Press. Guidebook on calcium binding proteins (in press), (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Miyahara: "Human group II phospholipase A2 in normal and diseased intervertebral discs" Biochim.Biophys.Acta. 1316. 183-190 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] "High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipoase C by the Bacillus brevis host-vector system" Jpn.J.Med.Sci.Biol.49. 103-112 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi,T.: "Replication of protein-A mediates the interaction between calf thymus DNA polymerase α-primase and a deoxyguanine-rich DNA sequence" J.Biochem.119. 766-772 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Liu,N.: "A new phospholipase C δ4 is induced at S-phase of cell cycle and appears in nucleus" J.Biol.Chem.271. 355-360 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukami,K.: "Identification of a PIP2-binding site in chicken skeletal muscle α-actinin" J.Biol.Chem.271. 2646-2650 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Banno,Y.: "Differential translocation of phospholipase C isozymes to integrin-mediated cytoskeletal complexes in thrombin-stimulated human platelets" J.Biol.Chem.271. 14989-14994 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kai,M.: "Identification and cDNA cloning of 35-kDa phosphatidic acid phosphatase (type 2) bound to plasma membranes." J.Biol.Chem.271. 18931-18938 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakane,F.: "Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein tyrosine kinases" J.Biol.Chem.271. 8394-8401 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kuge,O.: "Post-translational processing of the phosphatidylserine decarboxylase gene product in Chinese hamster ovary cells" Biochem.J.319. 33-38 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ho,Y-S.: "Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer" Nature. 385. 89-93 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Ishii,S.: "Bronchial hyperreactivity,increased endotoxin lethality,and melanocytic tumorigenesis in transgenic mice overexpressing platelet-activating factor receptor" EMBO J.16. 133-142 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohguchi,K.: "Enhancement of phospholipase D activity during differentiation in human promyelocytic leukemic HL60 cells" J.Biol.Chem.272. 1990-1996 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Higashida,H.: "Progress in Brain Research" Bradykinin B2 receptors and signal transduction analyzed in NG108-15 neuroblastoma x glioma hybrid cells,B2 receptor-transformed CHO cells and ras-transformed NIH3T3 fibroblasts (ed.Kruger,L.). Elsevier Science Publisher B.V., 15 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakashima,S.: "Advances in Molecular and Cell Biology Series,Vol.17,The Platelet" Role of signal-transducing phospholipase A2,C and D in platelet activation. JAI Press inc,Greenwich, 21 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakashima,S.: "Handbook of Experimental Pharmacology,"Platelets and their factors"" Platelet phospholipase C,D (Bruchhausen,V.and Walter,U.,eds.) Springer-Verlag,Berli, 15 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takenawa,T.: "Advance in Pharmacol,vol.36 Intracellular Signal Transduction" Signal transductions of SH2/SH3 : Ash/Grb2 downstream signals (eds.Narin and Hidaka), 14 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohguchi K.: "Regulation of membrane phospholipase D by protein kinase C in HL60 cells.Synergistic action of small GTP-binding rotein RhoA" J. Biol. Chem.271. 4366-4373 (1996)

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      1995 Annual Research Report
  • [Publications] Karasawa K.: "Arteriosclerosis and platelet-activating factor(PAF)-molecular biology of PAF-acetylhydrolase" Sugiyama Chemical and Industrial Laboratory Annual Report. 55-68 (1995)

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      1995 Annual Research Report
  • [Publications] Hanada K.: "Both sphingolipids and cholesterol participate in the detergent insolubility of a alkaline phosphatase, a glycosylphosphatidylinositol-anchored protein, in mammalian membranes" J. Biol. Chem.270. 6254-6260 (1995)

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      1995 Annual Research Report
  • [Publications] Liu,N.: "A new phospholipase Cd4 is induced at S-phase of the cell cycle and appears in the nucleus" J. Biol. Chem.271. 355-360 (1996)

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      1995 Annual Research Report
  • [Publications] Sakane F.: "Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein tyrosine kinases" J. Biol. Chem.(in press).

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      1995 Annual Research Report
  • [Publications] Takahashi T.: "Structural and thermotrpic properties of calcium-dimyristoylphosphatidic acid complexes at acidic and neutral pH conditions" Biophys. J.69. 1464-1472 (1995)

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      1995 Annual Research Report
  • [Publications] Hattori M.: "Cloning and expression of a cDNA encoding the b-subunit(30-kDa subunit)of bovine brain platelet-activating factor acetylhydrolase" J. Biol. Chem.270. 31345-31352 (1995)

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      1995 Annual Research Report
  • [Publications] Tomita M.: "Studies of the catalytic sites on glycosylphosphatidylinositol-anchoring aminopeptidase N from the larval midgut of the silkworm, Bombyx mori, and its expression virus-infected silkworm21GC08:Protein Engineering" 8. 947- (1995)

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      1995 Annual Research Report
  • [Publications] Sigiura T.: "Group II phospholipase A2 activates mitogen-activated protein kinase in cultured rat mesangial cells" FEBS Lett.370. 141-145 (1995)

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      1995 Annual Research Report
  • [Publications] Kawaguchi M.: "Selective inhibition of DNA polymerase e by phosphatidylinositol" J. Biochem.117. 1095-1099 (1995)

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      1995 Annual Research Report
  • [Publications] Watanabe T.: "Prostaglandin F2a stimulates formation of p21ras-GTP complex and mitogen-activated protein kinase in NIH-3T3 cells via Gq-protein-coupled pathway" J. Biol. Chem.270. 8984-8990 (1995)

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      1995 Annual Research Report
  • [Publications] Nojima,S.: "Antibodies to PAF and PAF‐associated diseases." J.Lipid Mediators Cell Signalling.10. 191-192 (1994)

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      1994 Annual Research Report
  • [Publications] Pugin,J.: "CD14 is a pattern recognition receptor." Immunity.1. 509-516 (1994)

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      1994 Annual Research Report
  • [Publications] Fukami,K.: "α‐Actinin and vinculin are PIP2‐binding proteins involved in signaling by tyrosine kinase." J.Biol.Chem.269. 1518-1522 (1994)

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      1994 Annual Research Report
  • [Publications] Kai,M.: "Molecular cloning of a diacylglycrerol kinase isozyma predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells." J.Biol.Chem.269. 18492-18498 (1994)

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      1994 Annual Research Report
  • [Publications] Hattori,M.: "Miller‐Diekerlissencephaly gene encodes a subunit of brain platelet‐activating factor." Nature.370. 216-218 (1994)

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      1994 Annual Research Report
  • [Publications] Taguchi,R.: "Microheterogeneity in glycosylphosphatidylinositol anchor structures of bovine liver 5′‐nucleotidase." Biochemistry.33. 1017-1022 (1994)

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      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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