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Structures and Formation Mechanisms of Folding Intermediates of Proteins

Research Project

Project/Area Number 06304051
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

KATAOKA Mikio  Osaka University, Faculty of Science, Associate Professor, 理学部, 助教授 (30150254)

Co-Investigator(Kenkyū-buntansha) KIHARA Hiroshi  Kansai Medical University, Physics Laboratory, Professor, 医学部, 教授 (20049076)
GEKKO Kunihiko  Hiroshima University, Faculty of Science, Professor, 理学部, 教授 (10023467)
KUWAJIMA Kunihiro  Tokyo University, Faculty of Science, Associate Professor, 理学系研究科, 助教授 (70091444)
AKASAKA Kazuyuki  Kobe University, Faculty of Science, Professor, 理学部, 教授 (50025368)
GOTO Yuji  Osaka University, Faculty of Science, Associate Professor, 理学部, 助教授 (40153770)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥9,900,000 (Direct Cost: ¥9,900,000)
Fiscal Year 1995: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1994: ¥5,100,000 (Direct Cost: ¥5,100,000)
Keywordsprotein folding / molten globule / X-ray solution scattering / protein denturation / high pressure NMR / stopped-flow CD / chaperonine / gene engineering / 蛋白質変性 / 変性 / 静電的相互作用 / 疎水的相互作用 / 蛋白質非天然構造
Research Abstract

Solution structures of molten globules (MG) of various proteins were investigated in detail by solution X-ray scattering. The structure of MG can be classified into two categories : one is close to native structure and the other is composed of a hydrophobic core and flaring tail (s) . Some MG's are stabilized mainly by hydrophobic interaction, the other MG's are stabilized mainly by intramolecular SS bonds. The denatured states cannot be generalized by a term, random coil, because the structural diversity and variety in the denatured states were also indicated.
Kinetic studies of beta-lactoglobulin folding demonstrated the accumulation of intermediate with non-native secondary structure, which suggests the importance of non-hierarchical model for folding. Isothermal titration calorimetric measurements on MG formation indicated that the hydrophobic interaction existed in MG is almost 40% of that in native state.
It was revealed that denaturant-induced denaturation of alpha-subunit of tryp … More tophane synthase is 3 states, however its thermal denaturation is 2 states. Kinetic studies on proline mutants indicated the existence of two successive intermediates in the folding process of alpha-subunit of tryptophane synthase. Proline residues are contributed to the stability of the late intermediate.
The mechanism of target recognition by chaperonine have been investigated using MG of alpha-lactalbumin to reveal the importance of electro-static interaction.
Theoretical studies were performed on the global structures of MG and the determinant, especially the contribution of water to the MG formation. Models of MG were proposed to various proteins and the calculated scattering profiles were compared with the observed ones. Consequently the proposed theoretical method was turned out to be quite promising for the folding studies.
High pressure NMR method for protein solution was developed and applied to thermal denaturation of RNase A.It was revealed that RNase A undergoes two-state transition even under high pressure. A volume change by denaturation was negative and also a heat capacity at constant pressure was decreased significantly by addition of pressure. Adiabatic compressibility upon denaturation was measured precisely. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (172 results)

All Other

All Publications (172 results)

  • [Publications] Mikio Kataoka: "Energy coupling in an ion pump : The reprotonation switch of bacteriorhodopsin" Journal of Molecular Biology. 243. 621-638 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Nishii: "Cold-denaturation of the molten globule state of apomyoglobin and a profile of protein folding" Biochemistry. 33. 4903-4909 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikio Kataoka: "Structural characterization of molten globule and native states of apomyoglobin by solution X-ray scattering" Journal of Molecular Biology. 249. 215-228 (1995)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Nishii: "Thermodynamic stability of the molten globule states of apomyoglobin" Journal of Molecular Biology. 250. 223-228 (1995)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Konno: "Small angle X-ray scattering analysis of cold-, heat-, and urea-denatured state in a protein, Streptomyces subtilisin inhibitor" Journal of Molecular Biology. 251. 95-103 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshinobu Izumi: "The chain dynamics of mastoparan bound to calmodulin" Physica. B213&214. 772-774 (1995)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasushi Imamoto: "Reconstitution of photoactive yellow protein from apoprotein and p-coumaric acid derivatives" FEBS Letters. 374. 157-160 (1995)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 片岡幹雄: "タンパク質のX線溶液散乱" 生物物理. 35. 239-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Daizo Hamada: "Role of heme axial ligands in the conformational stability of the native and molten globule states of horse cytochrome C" Journal of Molecular Biology. 256. 172-186 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikio Kataoka: "The linker of calmodulin lacking Glu84 is elongated in solution ; Although it is bent in the crystal" Proteins : Structure, Function and Genetetics. (印刷中). (1996)

    • Description
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    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hironari Kamikubo: "Structure of the N intermediate of bacteriorhodopsin revealed by X-ray diffraction" Proceedings of National Academy of Science, U. S. A.(印刷中). (1996)

    • Description
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    • Related Report
      1995 Final Research Report Summary
  • [Publications] Li Shi: "Conformational characterization of DnaK and its complexes with substate protein by small-angle X-ray scattering" Biochemistry. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Daizo Hamada: "Salt-induced formation of the molten globule state of cytochrome c studied by isothermal titration calorimetry" Proceedings of National Academiy of Science, U. S. A.91. 10325-10329 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshihisa Hagihara: "Comparison of the conformational stability of the molten globule and native states of horse cytochrome c" Journal of Molecular Biology. 237. 336-348 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshihisa Hagihara: "Thermal unfolding of tetrameric melittin : Comparison with the molten globule state of cytochrome c" Protein Science. 3. 1418-1429 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kentaro Shiraki: "Trifluoroethanol-induced stabilization of the α-helical structure of β-lactoglobulin : Implication for non-hierarchical protein folding" Journal of Molecular Biology. 245. 180-194 (1995)

    • Description
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    • Related Report
      1995 Final Research Report Summary
  • [Publications] Daizo Hama: "Salt-induced formation of the molten globule state of apomyoglobin studied by isothermal titration calorimetry" Thermochica Acta. 266. 385-400 (1995)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Daizo Hamada: "High helical propensity of the peptide fragments derived from β-lactoglobulin, a predominantly β-sheet protein" Journal of Molecular Biology. 254. 737-746 (1995)

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      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Effect of the order of antibody variable regions on the expression of the single-chain HYHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binnding properties" Biochemical and Biophysical Researcg Communication. 201. 546-551 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masafumi Odaka: "Tyr-341 of the β subunit is a major Km-determining residues of TF1-ATPase : Parallel effect its mutations on Kd(ATP)of the β subunit and on Km(ATP)of the α3β3γ complex" Journal of Biochemistry. 115. 789-796 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 油谷克英: "安定性" 蛋白質核酸酵素. 39. 1017-1026 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody binding" Journal of Biological Chemistry. 269. 28777-28782 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kyoko Ogasahara: "Unfolding-refolding Kinetics of the Tryptophan Synthase α Subunit by CD and fluorescence measurements" Journal of Molecular Biology. 236. 1227-1240 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kouhei Tsumoto: "Role of Tyr residues in the contact region of anti-lysozyme monoclonal antibody HyHEL10 for antigen binding" Journal of Biological Chemistry. 270. 18551-18557 (1995)

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      1995 Final Research Report Summary
  • [Publications] 油谷克英: "タンパク質の変性の熱力学" 生物物理. 35. 3-7 (1996)

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    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazufumi Takano: "Contribution of hydrophobic residues to the stability of human lysozyme" Journal of Molecular Biology. 254. 62-76 (1996)

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    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazufumi Yamazaki: "Folding pathway of Escherichia coli ribonuclease HI : A circular dichroism, fluorescence, and NMR study" Biochemistry. 34. 16552-16562 (1995)

    • Description
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    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tohru Yamaguchi: "Thermodynamics of Unfolding of Ribonuclease A Under High Pressure. A Study by Proton NMR" Journal of Molecular Biology. 250. 689-694 (1995)

    • Description
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    • Related Report
      1995 Final Research Report Summary
  • [Publications] Atsuo Tamura: "Dynamics of the three methionyl side chains of Streptomyces subtilisininhibitor. Deuterium NMR studies in solution and in solid" Protein Science. 5. 127-139 (1996)

    • Description
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    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaori Chiba: "Kinetic study on myoglobin refolding monitored by five optical stopped-flow methods" Protein : Structure, Function Genetics. 19. 110-119 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hideo Arakawa: "The Kinetics of conformational changes of α-2 macroglobulin determined by time resolved X-ray solution scattering" FEBS Letters. 337. 171-174 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroshi Kihara: "Stopped-flow apparatus for X-ray scattering and XAFS" Journal of Synchrotron Radiation. 1. 74-77 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirotsugu Tsuruta: "Kinetics of the quaternary structure change of aspartate transcarbamylase triggered by succinate, a competitive inhibitor" Biochemistry. 33. 10007-10012 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mamoru Sato: "The ATP-hydrolyzing excited structure of the reconstituted α3β3 complex of ATP synthase from thermophilic bacterium PS3 : the structural feature revealed by time-resolved small-angle x-ray scattering with synchrotron radiation" Journal of Biochemistry. 117. 113-119 (1995)

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      1995 Final Research Report Summary
  • [Publications] Yoshihiko Igarashi: "Solution X-ray scattering study on the chaperonin GroEL for Escherichia coli" Biophysical Chemistry. 53. 259-266 (1995)

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      1995 Final Research Report Summary
  • [Publications] Akira Okazaki: "The chaperonin GroEL does not recognize apo-alpha-lactalbumin in the molten globule state" Nature Structural Biology. 1. 439-446 (1994)

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      1995 Final Research Report Summary
  • [Publications] 桑島邦博: "蛋白質の折れ畳みの速度論" SR科学技術情報. 4(12). 2-10 (1994)

    • Description
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      1995 Final Research Report Summary
  • [Publications] 桑島邦博: "折れ畳み機構" 蛋白質核酸酵素. 39. 1011-1016 (1994)

    • Description
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      1995 Final Research Report Summary
  • [Publications] 桑島邦博: "球状タンパク質のモルテン・グロビュール状態" CBINews. 14. 11-13 (1994)

    • Description
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      1995 Final Research Report Summary
  • [Publications] N. N. Kalnin: "Kinetic folding and unfolding of staphylococcal nuclease and its six mutants studied by stopped-flow circular dichroism" Proteins : Structure, Function, Genetics. 23. 163-176 (1995)

    • Description
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      1995 Final Research Report Summary
  • [Publications] Akira Okazaki: "What is the molten globule? Reply" Nature Structure Biology. 2. 10-11 (1995)

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      1995 Final Research Report Summary
  • [Publications] Hidefumi Uchiyama: "Effects of amino acid substitutions in the hydrophobic core of α-lactalbumin on the stability of the molten globule state" Protein Engineering. (印刷中). (1995)

    • Description
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      1995 Final Research Report Summary
  • [Publications] Kunihiro Kuwajima: "The molten globule state of α-lactalbumin" FASEB Journal. 10. 102-109 (1996)

    • Description
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      1995 Final Research Report Summary
  • [Publications] Kumiko Katsumata: "Effect of GroEL on the refolding kinetics of α-lactalbumin" Journal of Molecular Biology. (印刷中). (1996)

    • Description
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    • Related Report
      1995 Final Research Report Summary
  • [Publications] 桑島邦博: "GroELによる標的タンパク質認識の分子機構" 蛋白質・核酸・酵素. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunihiko Gekko: "Point mutationa at glycine-121 of Escherichia coli dihydrofolate reductase : Important roles of a flexible loop in the stability and function" Journal of Biochemistry. 116. 34-41 (1994)

    • Description
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      1995 Final Research Report Summary
  • [Publications] 月向邦彦: "高圧下における食品高分子のゾル-ゲル転移" New Food Industry. 36. 68-79 (1994)

    • Description
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      1995 Final Research Report Summary
  • [Publications] Youjiro Tamura: "Compactness of thermally and chemically denatured ribonuclease A as revealed by volume and compressibility" Biochemistry. 34. 1878-1884 (1995)

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      1995 Final Research Report Summary
  • [Publications] 月向邦彦: "密度と部分比容の測定" 蛋白質・核酸・酵素. 40. 2461-2465 (1995)

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      1995 Final Research Report Summary
  • [Publications] Kunihiko Gekko: "A large compressibility change of protein induced by a single amino acid substitution" Protein Science. 5(印刷中). (1996)

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      1995 Final Research Report Summary
  • [Publications] Eiji Ohmae: "Effects of point mutation at a flexible loop glycine-67 of Escherichia coli dihydrofolate reductase on the stability and function" Journal of Biochemistry. (印刷中). (1996)

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      1995 Final Research Report Summary
  • [Publications] Kunitsugu Soda: "Transfer-thermodynamic quantities and the hydorphobic effect" Journal of Physical Society of Japan. 63. 814-824 (1994)

    • Description
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      1995 Final Research Report Summary
  • [Publications] 曽田邦嗣: "タンパク質の構造安定性の熱力学と疎水効果" ぶんせき. 1995(8). 630-637 (1995)

    • Description
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      1995 Final Research Report Summary
  • [Publications] Hideki Tsujishita: "Potential-scaled/hot-solute molecular dynamics for modeling of the three-dimensional structure of polypeptides in solution" Biophysical Journal. 66. 1815-1822 (1994)

    • Description
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  • [Publications] Kyohei Kato: "Solution sructure of HIV-1 protease P allophenylnorstatine derivative inhibitor obtained from molecular dynamics simulation" Chemical Pharmaceutical Bullettin. 42. 176-178 (1994)

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  • [Publications] Noriyuki Yamaotsu: "Solvation free energies of amino acids calculated by molecular dynamics/free energy perturbation method" Chemical Pharmaceutical Bullettin. 43. 717-721 (1995)

    • Description
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      1995 Final Research Report Summary
  • [Publications] M.Kataoka, H.Kamikubo, F.Tokunaga, L.S.Brown, Y.Yamazaki, A.Maeda, M.Sheves, R.Needleman & J.K.Lanyi: "Energy coupling in an ion pump : The reprotonation switch of bacteriorhodopsin" Journal of Molecular Biology. 243. 621-638 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] I.Nishii, M.Kataoka, F.Tokunaga & Y.Goto: "Cold-denaturation of the molten globule state of apomyoglobin and a profile of protein folding" Biochemistry. 33. 4903-4909 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] D.Hamada, H.Fukada, K.Takahashi & Y.Goto: "Salt-induced formation of the molten globule state of cytochrome c studied by isothermal titration calorimetry" Proc.Natl.Acad.Sci.U.S.A.91. 10325-10329 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Hagihara, Y.Tan & Y.Goto: "Comparison of the conformational stability of the molten globule and native states of horse cytochrome c.Effects of acetylation, heat, urea and guanidine hydrochlolide" Journal of Molecular Biology. 237. 336-348 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Hagihara, M.Oobatake & Y.Goto: "Thermal unfolding of tetrameric melittin : Comparison with the molten globule state of cytochrome c" Protein Science. 3. 1418-1429 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Tsumoto, Y.Nakaoki, Y.Ueda, K.Ogasahara, K.Yutani, K.Watanabe & I.Kumagai: "Effect of the order of antibody variable regions on the expression of the single-chain HYHEL10Fv fragment in E.coli and the thermodynamic analysis of its antigen-binding properties" Biochem.Biophys.Res.Comm.201. 546-551 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Odaka, C.Kaibara, T.Amano, T.Matsui, E.Muneyuki, K.Ogasahara, K.Yutani & M.Yoshida: "Tyr-341 of the beta subunit is a major Km-determining residues of TF1-ATPase : Parallel effect of its mutations on Kd (ATP) of the beta subunit and on Km (ATP) of the alpha3beta3gamma complex" Journal of Biochemistry. 115. 789-796 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Yutani: "Stability (in Japanese)" Protein, Nucleic Acid, Enzyme. 39. 1017-1026 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Tsumoto, Y.Ueda, K.Maenaka, K.Watanabe, K.Ogasahara, K.Yutani & I.Kumagai: "Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody binding" J.Biol.Chem.269. 28777-28782 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Ogasahara & K.Yutani: "Unfolding-refolding kinetics of the tryptophan synthase alpha subunit by CD and fluorescence measurements" Journal of Molecular Biology. 236. 1227-1240 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Chiba, A.Ikai, Y.Kawamura-Konishi, D.Eliezer & H.Kihara: "Kinetic study on myoglobin refolding monitored by five optical stopped-flow methods" Proteins : Struct.Funct.Genet.19. 110-119 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Arakawa, T.Urisaka, H.Tsuruta, Y.Amemiya, H.Kihara & A.Ikai: "The Kinetics of conformational changes of alpha-2 mecroglobulin determined by time resolved X-ray solution scattering" FEBS Letters. 337. 171-174 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kihara: "Stopped-flow apparatus for x-ray scattering and XAFS" J.Synchrotron Rad.1. 74-77 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Tsuruta, P.Vachette, T.Sano, M.F.Moody, Y.Amemiya, K.Wakabayashi & H.Kihara: "Kinetics of the quaternary structure change of aspartate transcarbamylase triggered by succinate, a competitive inhibitor" Biochemistry. 33. 10007-10012 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Okazaki, T.Ikura, K.Nikaido & K.Kuwajima: "The chaperonin GroEL does not recognize apo-alpha-lactalbumin in the molten globule state" Nature Struct.Biol.1. 439-446 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kuwajima & T.Ikura: "Folding Mechanism (in Japanese)" Protein, Nucleic Acid, Enzyme. 39. 1011-1016 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kuwajima: "Molten globule states of globular proteins (in Japanese)" CBI News. 14. 11-13 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Gekko, Y.Kunori, H.Takeuchi, S.Ichihara & M.Kodama: "Point mutationa at glycine-121 of Escherichia coli dihydrofolate reductase : Important roles of a flexible loop in the stability and function" Journal of Biochemistry. 116. 34-41 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Gekko: "Sol-gel transition of food polymer under high pressure (in Japanese)" New Food Industry. 36. 68-79 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Soda & N.Tsuruta: "Transfer-thermodynamic quantities and the hydorphobic effect" J.Phys.Soc.Jpn.63. 814-824 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Tsujishita, I.Moriguchi & S.Hirono: "Potential-scaled/hot-solute molecular dynamics for modeling of the three-dimensional structure of polypeptides in solution" Biophysical Journal. 66. 1815-1822 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] R.Kato, O.Takahashi, Y.Kiso, I.Moriguchi & S.Hirono: "Solution structure of HIV-1 protease P allophenylnorstatine derivative inhibitor obtained from molecular dynamics simulation" Chem.Pharm.Bull.42. 176-178 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Kataoka, I.Nishii, T.Fujisawa, T.Ueki, F.Tokunaga & Y.Goto: "Structural characterization of molten globule and native states of apomyoglobin by solution X-ray scattering" Journal of Molecular Biology. 249. 215-228 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] I.Nishii, M.Kataoka & Y.Goto: "Thermodynamic stability of the molten globule states of apomyoglobin" Journal of Molecular Biology. 250. 223-228 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Konno, M.Kataoka, Y.Kamatari, K.Kanaori, A.Nosaka & K.Akasaka: "Small angle X-ray scattering analysis of cold-, heat-, and urea-denatured states in a protein, Streptomyces subtilisin inhibitor" Journal of Molecular Biology. 251. 95-103 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Izumi, K.Sakai, H.Yoshino & M.Kataoka: "The chain dynamics of mastoparan bound calmodulin" Physica. B213&214. 772-774 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Imamoto, T.Ito, M.Kataoka & F.Tokunaga: "Reconstitution of photoactive yellow protein from apoprotein and p-coumaric acid derivatives" FEBS Letters. 374. 157-160 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Kataoka: "X-ray solution scattering of proteins (in Japanese)" Seibutsu-butsuri. 35. 239-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Shiraki, K.Nishikawa & Y.Goto: "Trifluoroethanol-induced stabilization of the alpha-helical structure of beta-lactoglobulin : Implication for non-hierarchical protein folding" Journal of Molecular Biology. 245. 180-194 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] D.Hamada, H.Fukuda, K.Takahashi & Y.Goto: "Salt-induced formation of the molten globule state of apomyoglobin studied by isothermal titration calorimetry" Thermochica Acta. 266. 385-400 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] D.Hamada, Y.Kuroda, T.Tanaka & Y.Goto: "High helical propensity of the peptide fragments derived from beta-lactoglobulin, a predominantly beta-sheet protein : Implication for non-hierarchical protein folding" Journal of Molecular Biology. 254. 737-746 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Tsumoto, K.Ogasahara, Y.Ueda, K.Watanabe, K.Yutani & I.Kumagai: "Role of Tyr residues in the contact region of anti-lysozyme monoclonal antibody HyHEL10 for antigen binding" J.Biol.Chem.270. 18551-18557 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Yutani: "Thermodynamics of protein denaturation (in Japanese)" Seibutsubutsuri. 35. 3-7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Takano, K.Ogasahara, H.Kaneda, Y.Yamagata, S.Fujii, E.Kanaya, M.Kikuchi, M.Oobatake & K.Yutani: "Contribution of hydrophobic residues to the stability of human lysozyme : Calorimetric studies and X-ray structural analysis of the five isoleucine to valine mutants" Journal of Molecular Biology. 254. 62-76 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Yamazaki, K.Ogasahara, K.Yutani, M.Oobatake & S.Kanaya: "Folding pathway of Escherichia coli ribonuclease HI : A circular dichroism, fluorescence, and NMR study" Biochemistry. 34. 16552-16562 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Yamaguchi, H.Yamada & K.Akasaka: "Thermodynamics of unfolding of ribonuclease A under high pressure. A study of proton NMR" Journal of Molecular Biology. 250. 689-694 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Sato, Y.Ito, M.Harada, H.Kihara, H.Tsuruta, S.Ohta & Y.Kagawa: "The ATP-hydrolyzing excited structure of the reconstituted alpha3beta3 complex of ATP synthase from thermophilic bacterium PS3 : the structural feature revealed by time-resolved small-angle x-ray scattering with synchrotron radiation" Journal of Biochemistry. 117. 113-119 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Igarashi, K.Kimura, K.Ichimura, S.Matsuzaki, T.Ikura K.Kuwajima & H.Kihara: "Solution X-ray scattering study on the chaperonin GroEL for Escherichia coli" Biophysical Chemistry. 53. 259-266 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.N.Kalnin & K.Kuwajima: "Kinetic folding and unfolding of staphylococcal nuclease and its six mutants studied by stopped-flow circular dichroism" Proteins : Struct.Funct.Genet.23. 163-176 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Okazaki, T.Ikura & K.Kuwajima: "What is the molten globule? Reply" Neture Struct.Biol.2. 10-11 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Uchiyama, E.M.Perez-Prat, K.Watanabe, I.Kumagai & K.Kuwajima: "Effects of amino acid substitutions in the hydrophobic core of alpha-lactalbumin on the stability of the molten globule state" Protein Engineering. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Tamura & K.Gekko: "Compactness of thermally and chemically denatured ribonuclease A as revealed by volume and compressibility" Biochemistry. 34. 1878-1884 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Gekko: "Measurements of density and partial specific volume (in Japanese)" Protein, Nuclecic Acid, Enzyme. 40. 2461-2465 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Soda: "Thermodynamics of the structural stability of proteins and the hydrophobic effect (in Japanese)" Bunseki. 1995 (8). 630-637 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Yamaotsu, I.Moriguchi & S.Hirono: "Solvation free energies of amino acids calculated by molecular dynamics/free energy perturbation method" Chem.Pharm.Bull.43. 717-721 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] D.Hamada, Y.Kuroda, M.Kataoka, S.Aimoto, T.Yoshimura & Y.Goto: "Role of heme axial ligands in the conformational stability of the native and molten globule states of horse cytochrome c" Journal of Molecular Biology. 256. 172-186 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Kataoka, A.Persechini, F.Tokunaga & R.H.Kretsinger: "The linker of calmodulin lacking Glu84 is elongated in solution ; Although it is bent in the crystal" Proteins : Struct, Funct, Genet. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kamikubo, M.Kataoka, G.Varo, T.Oka, F.Tokunaga, R.Needleman & J.K.Lanyi: "Structure of the N intermediate of bacteriorhodopsin revealed by X-ray diffraction" Proc.Natl.Acad.Sci.U.S.A.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] L.Shi, M.Kataoka & A.L.Fink: "Conformational characterization of DnaK and its complexes with substrate protein by small-angle X-ray scattering" Biochemistry. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Tamura, M.Matsushita, A.Naito, S.Kojima, K.-I.Miura & K.Akasaka: "Dynamics of the three methionyl side chains of Streptomyces subtilisininhibitor. Deuterium NMR studies in solution and in solid" Protein Science. 5. 127-139 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kuwajima: "The molten globule state of alpha-lactalbumin" FASEB Journal. 10. 102-109 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Katsumata, A.Okazaki & K.Kuwajima: "Effect of GroEL on the refolding kinetics of alpha-lactalbumin" Journal of Molecular Biology. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kuwajima: "Molecular mechanism of target protein recognition by GroEL (in Japanese)" Protein, Nucleic Acid, Enzyme. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Gekko, Y.Tamura, E.Ohmae, H.Hayashi, H.Kagamiyama & H.Ueno: "A large compressibility change of protein induced by a single amino acid substitution" Protein Science. 5 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] E.Ohmae, K.Iriyama, S.Ichihara & K.Gekko: "Effects of point mutation at a flexible loop glycine-67 of Escherichia coli dihydrofolate reductase on the stability and function" Journal of Biochemistry. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mikio Kataoka: "Structural characterization of molten globule and native states of apomyoglobin by solution X-ray scattering" Journal of Molecular Biology. 249. 215-228 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Nishii: "Thermodynamic stability of the molten globule states of apomyoglobin" Journal of Molecular Biology. 250. 223-228 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takashi Konno: "Small angle X-ray scattering analysis of cold-,heat-,and urea-denatured state in a protein,Streptomycessubtilisin inhibitor" Journal of Molecuar Biology. 251. 95-103 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshinobu Izumi: "The chain dynamics of mastoparan bound to calmodulin" Physica. B213&214. 772-774 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasushi Imamoto: "Reconstitution of photoactive yellow protein from apoprotein and p-coumaric acid deribatives" FEBS Letters. 374. 157-160 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 片岡幹雄: "タンパク質のX線溶液散乱" 生物物理. 35. 239-241 (1995)

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      1995 Annual Research Report
  • [Publications] Daizo Hamada: "Role of heme axial ligands in the conformational stability of the native and molten globule states of horse cytochrome c" Journal of Molecular Biology. 256. 172-186 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mikio Kataoka: "The linker of calmodulin lacking Glu84 is elongated in solution;Although it is bent in the crystal" Proteins:Structure,Function and Genetetics. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hironari Kamikubo: "Structure of the N intermediate of bacteriorhodopsin revealed by X-ray diffraction" Proceedings of National Academy of Science,U.S.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Lishi: "Conformational characterization of Dnak and its complexes with substrate protein by small-angle X-ray scatterig" Biochemistry. (印刷中). (1996)

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      1995 Annual Research Report
  • [Publications] Kentaro Shiraki: "Trifluoroethanol-induced stabilization of the α-helical structure of β-lactoglobulin:Implication for non-hierarchical protein folding" Journal of Molecular Biology. 237. 336-348 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Daizo Hama: "Salt-induced formation of the molten globule state of apomyoglobin studied by isothermal titration calorimetry" Thermochica Acta. 226. 385-400 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Daizo Hamada: "High helical propensity of the peptide fragments derived from β-lactoglobulin,a predominantly β-sheet protein:Implication for non-hierarchical protein folding" Journal of Molecular Biology. 254. 737-746 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kouhei Tsumoto: "Role of Tyr residues in the contact region of anti-lysozyme monoclonal antibody HyHEL10 for antigen binding" Journal of Biological Chemistry. 270. 18551-18557 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 油谷克英: "タンパク質の変性の熱力学" 生物物理. 35. 3-7 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazufumi Takano: "Contribution of hydrophobic residues to the stability of human lysozyme:Calorimetric studies and X-ray structural analysis of the five Ile to Valmutants" Journal of Molecular Biology. 254. 62-76 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuhiko Yamazaki: "Folding pathway of Escherichia coli ribonuclease HI:A circular dichroism,fluorescence,and NMR study" Biochemistry. 34. 16552-16562 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Youjiro Tamura: "Compactness of thermally and chemically denatured ribonuclease A as revealed by volume and compressibility" Biochemistry. 34. 1878-1884 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 月向邦彦: "密度と部分比容の測定" 蛋白質・核酸・酵素. 40. 2461-2465 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kunihiko Gekko: "A large compressibility change of protein induced by a single amino acid substitution" Protein Science. 5(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Eiji Ohmae: "Effects of point mutation at a flexible loop glycine-67 of Escherichia coli dihydrofolate reductase on the stability and function" Journal of Biochemistry. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mamoru Sato: "The ATP-hydrolyzing excited structure of the reconstituted α3β3 complex of ATP synthase from thermophilic bacterium PS3:the structural feature revealed by time-resolved small-angle x-ray scattering with synehrotron radiation" Journal of Biochemistry. 117. 113-119 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshihiko Igarashi: "Solution X-ray scattering study on the chaperonin GroEL for Escherichia coli" Biophysical Chemistry. 53. 259-266 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N.N.Kalnin: "Kinetic folding and unfolding of staphylococcal nuclease and its six mutants studied by stopped-flow circular dichroism" Proteins:Structure,Functionh,Genetics. 23. 163-176 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Akira Okazaki: "What is the molten globule?Reply" Nature Structure Biology. 2. 10-11 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hidefumi Uchiyama: "Effects of amino acid substitutions in the hydrophobic core of α-lactalbumin on the stability of the molten globule state" Protein Engineering. (印刷中). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kunihiro Kuwajima: "The molten globule state of α-lactalbumin" FASEB Journal. 10. 102-109 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kumiko Katsumata: "Effect of GroEL on the refolding kinetics of α-lactalbumin" Journal of Molecular Biology. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 桑島邦博: "GroELによる標的タンパク質認識の分子機構" 蛋白質・核酸・酵素. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Noriyuki Yamaotsu: "Solvation free energies of amino acids calculated by molecular dynamics/free energy perturbation method" Chemical Pharmacology Bullettin. 43. 717-721 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tohru Yamaguchi: "Thermodynamics of unfolding of ribonuclease A under high pressure.A study by Proton NMR" Journal of Molecular Biology. 250. 689-694 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Atsuo Tamura: "Dynamics of the three methionyl side chains of Streptomyces subtilisininhibitor Deuterium NMR studies in solution and in solid" Protein Science. 5. 127-139 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 曽田邦嗣: "タンパク質の構造安定性の熱力学と疎水効果" ぶんせき. 1995(8). 630-637 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Nishii: "Cold-denaturation of the molten globule state of apomyoglobin and a profile of protein folding" Biochemistry. 33. 4903-4909 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mikio Kataoka: "Energy coupling in an ion pump:The reprotonation switch of bacteriorhodopsin" Journal of Molecular Biology. 243. 621-638 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mikio Kataoka: "Structural characterization of molten globule and native stares of apomyoglobin by solution X-ray scattering" Journal of Molecular Biology. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshihisa Hagihara: "Comparison of the conformational stability of the molten globule and native states of horse cytochrome c:Effects of acetylation,heat,urea and guanidine-hydrochloride" Journal of Molecular Biology. 237. 336-348 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Daizo Hamada: "Salt-induced formation of the molten globule state of cytochrome c studied by isothermal titration calorimetry" Proceedings of National Academy of Science U.S.A.91. 10325-10329 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yohihisa Hagihara: "Thermal unfolding of tetrameric melittin:Comparison with the molten globule state of cytochrome c" Protein Science. 3. 1418-1429 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masaru Hoshino: "Perchlorate-induced formation ofthe α-helical structure of mastoparan" Journal of Biochemistry. 116. 910-915 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kentaro Shiraki: "Trifluoroethanol-induced stabilizaytion of the α-helical structure of β-lactoglobulin:Implication for non-hierarchical protein folding" Journal of Molecular Biology. 245. 180-194 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kouhei Tsumoto: "Contribution to antibody-antigen interaction of structurally purterbed anatigenetic residues upon antibody binding" Journal of Biological Chemistry. 269. 28777-28782 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kouhei Tsumoto: "Effect of the order of antibody variable regions on the expression of the single-chain hyhe110 FV fragment in E,coli and the thermodynamic analysis of its antigen binding properities" Biochemical Biophysical Research Communication. 201. 546-551 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Odaka: "Tyr-341 of the beta subunit is a major Km-determining residue of TF1-ATPase:Parallel effect of its mutations on Kd(ATP)of the β subunit and on Km(ATP)of the α3β3γ complex" Journal of Biochemistry. 115. 789-796 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kyoko Ogasahara: "Unfolding-refolding kinetics of the tryptophan synthase alpha subunit by the CD and fluorescence measurements" Journal of Molecular Biology. 236. 1227-1240 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazuyuki Akasaka: "Local order,conformation,and interaction in nematic 4-n-pentyloxy-4'-cyanobiphenyl and its one-to-one mixture with 1-(4'-cyanophenyl)-4-propylcyclohexane" Journal of Physical Chemistry. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazuyuki Akasaka: "Three-dimensional structure of gurumarin,a sweet taste-suppressing polypeptide" Jounal of Biomolecular NMR. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hideo Arakawa: "Conformational changes of α-2 macroglobulin determined by time resolved X-ray solution scattering" FEBS Letters. 337. 171-174 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kaori Chiba: "Kinetic study on myoglobin refolding monitored by five optical stopped-flow methods" Proteins. 19. 110-119 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiroshi Kihara: "Stoped-flow apparatus for X-ray scattering and XAFS" Journal of Synchrotron Radiation. 1. 74-77 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hirotsugu Tsuruta: "Kinetics of the quartemary structure change of aspartate transcarbamylase triggered by succinate a competitive inhibitor" Biochemistry. 33. 10007-10012 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshihiko Igarashi: "Solution X-ray scattering study on the chaperonin GroEL for Escherichia coli" Biophysical Chemistry. 53. 259-266 (1995)

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      1994 Annual Research Report
  • [Publications] Akira Okazaki: "The chaperonin GroEL does not recognize apo-α-lactalbumin in the molten globule state" Nature:Structural Biology. 1. 439-446 (1994)

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      1994 Annual Research Report
  • [Publications] 桑島邦博: "折れ畳み機構" 蛋白質核酸酵素. 39. 1011-1016 (1994)

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      1994 Annual Research Report
  • [Publications] Kunihiko Gekko: "Point mutations at glycine-121 of Escherichia coli dehydrofolate reductase:Important roles of a flexible loop in the stability and function" Journal of Biochemistry. 116. 34-41 (1994)

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      1994 Annual Research Report
  • [Publications] Yoichi Tamura: "Compactness of thermally and chemically denatured ribonclease A as revealed by volume and compressibility" Biochemistry. 34. 1878-1884 (1994)

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      1994 Annual Research Report
  • [Publications] 月向邦彦: "高圧下における食品高分子のゾル-ゲル転移" New Food Industry. 36. 68-79 (1994)

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      1994 Annual Research Report
  • [Publications] Kunitsugu Soda: "Transfer-thermodynamic quantities and the hydrophobic effect" Journal of Physical Society of Japan. 63. 814- (1994)

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      1994 Annual Research Report
  • [Publications] 曽田邦嗣: "蛋白質の構造安定性の熱力学と疎水効果" ぶんせき. 4(印刷中). (1995)

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      1994 Annual Research Report
  • [Publications] Shuichi Hirono: "Non-congeneric structure-pharmacokinetic property correlation studies using fuzzy adaptive least-squares″:Volume of distribution" Biol.Pharm.Bulletin. 17. 686-690 (1994)

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      1994 Annual Research Report
  • [Publications] H.Tsujishita: "Potential-scaled/hot-solute molecular dynamics for modeling of the three-demensional structure of polypeptides in solution" Biophysical Journal. 66. 1815-1822 (1994)

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Published: 1994-04-01   Modified: 2016-04-21  

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