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Elucidation of Reaction Mechanisms of Chain-elongation Enzymes in Isoprenoid Biosynthesis

Research Project

Project/Area Number 06403033
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

OGURA Kyozo  Tohoku Univ., Institute for Chemical Reaction Science Professor, 反応化学研究所, 教授 (80006303)

Co-Investigator(Kenkyū-buntansha) SAGAMI Hiroshi  Tohoku Univ., Institute for Chemical Reaction Science Associate Professor, 反応化学研究所, 助教授 (10134058)
KOYAMA Tanetoshi  Tohoku Univ., Department of Biochemistry and Engineering Associate Professor, 工学部, 助教授 (20089808)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥30,900,000 (Direct Cost: ¥30,900,000)
Fiscal Year 1996: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1995: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1994: ¥23,700,000 (Direct Cost: ¥23,700,000)
KeywordsIsoprenoid / Biosynthesis / Farnesyl Diphosphate Synthase / Geranylgeranyl Diphosphate Synthase / Prenyltransferase / Heptaprenyl Diphosphate Synthase / Isopentenyl Diphosphate Synthase / Terpenoid
Research Abstract

1. Site-directed mutations were introduced into thermostable farnesyl diphosphate synthase of Bacillus stearothermophilus, and structure-reactivity relation was investigated. As a result, it has been demonstrated that Phe-220 and Gln-221 in the conserved motif FQXXDD are both essential for catalysis and that Lys-47 and Lys-183 are involved in the binding of isopentenyl diphosphate. Coupled together, these findings have led to a better understanding of the mechanism of prenyltransferase reaction.
2. The genes for the two components of B.stearothermophilus heptaprenyl diphosphate synthase were cloned, sequenced, and overexpressed. The gene encoding a methyltransferease responsible for the final step in menaquinone biosynthesis was found to be located between the two genes for heptaprenyl diphosphate synthase.
3. The purification of geranylgeranyl diphosphate synthase from bovine brain was achived, and the catalytic function was clarified.
4. A novel type of protein modification by isoprenoid derived materials was discovered by investigating the metabolism of mevalonate in halobacterium halobium.
5. Farnesol phosphokinase was found in Botryococcus braunii and its properties was disclosed.
6. A new metabolic system for the conversion of dolichols and dehydrodolichols into their aldehydes was discovered.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (116 results)

All Other

All Publications (116 results)

  • [Publications] H.Sagami et al.: "Purification and Properties of Geranylgeranyl Diphosphate Synthase from Bovine Brain" J.Biol.Chem.269. 20561-20566 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Inoue et al.: "Phosphorylation of Farnesol by a Cell-free System from Botryococcus Braunii" Biochem.Biophys.Res.Commun.200. 1036-1041 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Inoue et al.: "Formation of Farnesyl Oleate and Three Other Farnesyl Fatty Acid Esters by Cell-free Extracts from Botryococcus Braunii B Race" Phytochemistry. 36. 1203-1207 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Site-directed Mutagenesis of Farnesyl Diphosphate Synthase:Effect of Substitution on the Three Carboxyl Terminal Amino Acids" Can.J.Chem.72. 75-79 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Thermostable Farnesyl Diphosphate Synthase of Bacillus stearothermophilus:Crystallization and Site-directed Mutagenesis" Acta Biochim.Polonica.41. 281-292 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "Purification of Geranylgeranyl Diphosphate from Bovine Brain" Acta Biochim.Polonica.41. 293-302 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Maki et al.: "Farnesyl Diphosphate Synthase Reactions of Geranyl Diphosphate Analogues Having Oxygen Atoms in Their Alkyl Chains" Chem.Lett.1841-1844 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Structural and Functional Roles of the Cysteine Residues of Bacillus stearothermophilus Farnesyl Diphosphate Synthase" Biochemistry. 33. 12644-12648 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "Novel Isoprenoid Modified Proteins in Halobacteria" Biochem.Biophys.Res.Commun.203. 972-976 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Maki et al.: "Farnesyl Diphosphate Synthase Reaction with Dimethylallyl Diphosphate Analogues Having Oxygen Atoms in Their Chains" Chem.Lett.389-390 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H Sagami et al.: "A Novel Type of Protein Modification by Isoprenoid-derived Materials" J.Biol.Chem.270. 14851-14854 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Koike-Takeshita et at.: "Molecular Cloning and Nucleotide Sequences of the Genes for Two Essential Proteins Constituting a Novel Enzyme System for Heptaprenyl Diphosphate Synthesis" J.Biol.Chem.270. 18396-18400 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Significance of Phe-220 and Gln-221 in the Catalytic Mechanism of farnesyl Diphosphate Synthase of Bacillus stearothermophilus" Biochem.Biophys.Res.Commun.212. 681-686 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Maki et al.: "Substrate Specificity of Farnesyl Diphosphate Synthase from Bacillus stearothermophilus" Bioorg.Med.Chem.Lett.5. 1605-1608 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Inoue et al.: "Properties of Farnesol Phosphokinase of Botryococcus braunii" Phytochemistry. 40. 377-381 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "Enzymatic Formation of Dehydrodolichal and Dolichal,New Products Related to Yeast Dolichol Biosynthesis" J.Biol.Chem.271. 9560-9566 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Identification of Significant Residues in the Substrate Binding Site of Farnesyl Diphosphate Synthase" Biochemistry. 35. 9533-9538 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Ohnuma et al.: "Conversion from Farnesyl Diphosphate Synthase to Geranylgeranyl Diphosphate Synthase by Random Chemical Mutagenesis" J.Biol.Chem.271. 10087-10095 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kurisaki et al.: "Proteolytic Release of Dehydrodolichyl Diphosphate Synthase from Pig Testis Microsome" Biosci.Biotech.Biochem.60. 1109-1114 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Isopentenyl Diphosphate Isomerase in Rubber Latex" Phytochemistry. 43. 769-772 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kurisaki et al.: "Distribution of Polyprenols and Dolichols in Soybean Plant" Phytochemistry. 44. 45-50 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Koike-Takeshita et al.: "Identification of Novel Gene Cluster Participating in Menaquinone(Vitamin K_2)Biosynthesis.Clounng and Sequence Determination of the 2-Heptaprenyl-1,4-naphthoquinone Methyltransferase Gene of Bacillus stearothermophilus" J.Biol.Chem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Zhan et al.: "Two Cistrons of the gerC Operon of Bacillus subtilis Encode the Two Subunits Heptaprenyl Diphosphate Synthase" J.Bacteriol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Ohkura et al.: "A Partial Defeciency of Dehydrodolichol Reduction is a Cause of Carbohydrate-Defecient Glycoprotein Syndrome Type I" J.Biol.Chem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐上 博、小倉協三: "プレニル化蛋白質の発見とイソプレノイド生化学の新展開" 生化学, 14 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 小倉協三: "酵素研究の新展開(一島英治他編):イソプレノイド生合成酵素の分子機構" 東京化学同人, 12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 小倉協三: "有機化学のフロンティア(日本化学研究会編):イソプレノイドの生合成" 培風館, 22 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 小倉協三: "酵素による炭素・炭素結合形成反応と有機合成への応用" 有機合成化学会誌, 11 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ogura,T.Koyama: "Mechanistic Enzymology and Molecular Genetics of Chain Elongation in Isoprenoid Biosynthesis" Kodansha Scientific, 23 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ogura et al.: "Polyprenyl Diphosphate Synthases" Plenum Publishing Corporation(in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "Purification and Properties of Geranylgeranyl Diphosphate Synthase from Bovine Brain" J.Biol.Chem.269. 20561-20566 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Inoue et al.: "Phosphorylation of Farnesol by a Cell-free System from Botryococcus Braunii" Biochem.Biophys.Res.Commun.200. 1036-1041 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Inoue et al.: "Formation of Farnesyl Oleate and Three Other Farnesyl Fatty Acid Esters by Cell-free Extracts from Botryococcus Braunii B Race" Phytochemistry. 36. 1203-1207 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Site-directed Mutagenesis of Farnesyl Diphosphate Synthase : Effect of Substitution on the Three Carboxyl Terminal Amino Acids" Can.J.Chem.72. 75-79 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Thermostable Farnesyl Diphosphate Synthase of Bacillus stearothermophilus : Crystallization and Site-directed Mutagenesis" Acta Biochem.Polonica. 41. 281-292 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "Phrification of Geranylgeranyl Diphosphate from Bovine Brain" Acta Biochim.Polonica.41. 293-302 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Maki et al.: "Farnesyl Diphosphate Synthase Reactions of Geranyl Diphosphate Analogues Having Oxygen Atoms in Their Alkyl Chains" Chem.Lett.1841-1844 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Structural and Functional Roles of the Cysteine Residues of Bacillus stearothermophilus Farnesyl Diphosphate Synthase" Biochemistry. 33. 12644-12648 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "Novel Isoprenoid Modified Proteins in Halobacteria" Biochem.Biophys.Res.Commun.203. 972-976 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Maki et al.: "Farnesyl Diphosphate Synthase Reaction with Dimenthylally Diphosphate Analogues Having Oxygen Atoms in Their Chains" Chem.Lett.389-390 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "A Novel Type of Protein Modification by Isoprenoid-derived Materials" J.Biol.Chem.270. 14851-15854 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Koike-Takeshita et al.: "Molecular Cloning and Nucleotide Sequences of the Genes for Two Essential Proteins Constituting a Novel Enzyme System for Heptaprenyl Diphosphate Synthesis." J.Biol.Chem.270. 18396-18400 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Significance of Phe-220 and Gln-221 in the Catalytic Mechanism of farnesyl Diphosphate Synthase of Bacillus stearothermophilus" Biochem.Biophys.Res.Commun.212. 681-686 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Maki et al.: "Substrate Specificity of Farnesyl Diphosphate Synthase from Bacillus stearothermophilus" Bioorg.Med.Chem.Lett.5. 1605-1608 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Inoue et al.: "Properties of Farnesol Phosphokinase of Botryococcus braunii" Phytochemistry. 40. 377-381 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "Enzymatic Formation of Dehydrodolichal and Dolichal, New Products Related to Yeast Dolichol Biosynthesis" J.Biol.Chem.271. 9560-9566 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Identification of Significant Residues in the Substrate Binding Site of Farnesyl Diphosphate Synthase" Biochemistry. 35. 9533-9538 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Ohnuma et al.: "Conversion from Farnesyl Diphosphate Synthase to Geranylgeranyl Diphosphate Synthase by Random Chemical Mutagenesis" J.Biol.Chem.271. 10087-10095 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kurisaki et al.: "Proteolytic Release of Dehydrodolichyl Diphosphate Synthase from Pig Testis Microsomes" Biosci.Biotech.Biochem.60. 1109-1114 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Koyama et al.: "Isopentenyl Diphosphate Isomerase in Rubber Latex" Phytochemistry. 43. 769-772 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kurisak et al.: "Distribution of Polyprenols and Dolichols in Soybean Plant" Phytochemistry. 44. 45-50 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Koike-Takeshita et al.: "Identification of Novel Gene Cluster Participating in Menaquinone (Vitamin K_2) Biosynthesis. Cloning and Sequence Determination of the 2-Heptaprenyl-1,4-naphthoquinone Methyltransferase Gene of Bacillus stearothermophilus" J.Biol.Chem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Zhang et al.: "Two Cistrons of the gerC Operon of Bacillus subtilis Encode the Two Subunits Heptaprenyl Diphosphate Synthase" J.Bacteriol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Ohkura et al.: "A Partial Defeciency of Dehydrodolichol Reduction is a Cause of Carbohydrate-Defecient Glycoprotein Syndrome Type I" J.Biol.Chem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sagami et al.: "Discovery of Prenylated Proteins and New Aspects in Isoprenoid Biochemistry" Biochemistry. 66. 1488-1501 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ogura: "Carbon-Carbon Bond Forming Reactions by Enzymes and Their Application to Organic Synthesis" J.Synt.Org.Chem.Japan.54. 1034-1044 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ogura: New Aspects in Enzymology : Molecular Mechanisms of Enzymes in Isoprenoid Biosynthesis. Tokyokagakudojin, 58-69 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ogura: Frontiers in Organic Chemistry : Biosynthetic Chemistry on Isoprenoids. Baifukan, 156-177 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ogura et al.: Mechanistic Enzymology and Molecular Genetics of Chain Elongation in Isoprenoid Biosynthesis "Dynamic Aspects of Natural Products Chemistry" K.Ogura and U.Sankawa ed.Kidansha Scientific, Tokyo, 1-23 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ogura et al.: Polyprenyl Diphosphate Synthases "Subcellular Biochemistry, Vol.28, Cholesterol : Its Functions and Metabolism in Biology and Midicine" J.R.Harris ed.Plenum Publishing Corporation, London, (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Sagami at al: "Purification and Properties of Geranylgeranyl Diphoshate Synthase from Bovine Brain" J. Biol. Chem. 269. 20561-20566 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Inoue et al: "Phosphorylation of Farnesol by a Cell-free System from Botryococcus Braunii" Biochem. Biophys. Res. Commun.200. 1036-1041 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Inoue et al: "Formation of Farnesyl Oleate and Three Other Farnesyl Fatty Acid Esters by Cell-free" Plrytochemistry. 36. 1203-1207 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Koyama et al: "Site-directed Mutagenesis of Farnesyl Diphosphate Synthase : Effect of Substitution on the Three Carboxyl Terminal Amino Acids" Can. J. Chem. 72. 75-79 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Koyama et al: "Thermostable Farnesyl Disphoshate Synthase of Bacillus Stearothermophilus : Crystallization and Site-directed Mutagenesis" Acta Biochim. Polonica.41. 281-292 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Sagami et al.: "Purifation of Geranylgeranyl Diphoshate from Bovine Brain" Acta Biochim. Polonica.41. 293-302 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Maki et al: "Farnesyl Diphoshate Synthase Reactions of Geranyl Diphosphate Analogues Having Oxygen Atoms in Their Alkyl Chains" Chem. Lett.1841-1844 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Koyama et al: "Structural and Functional Roles of the Cysteine Residues of Bacillus stearothermophilus Farnesyl Disphoshase Synthase" Biochemistry. 33. 12644-12648 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Sagami et al: "Novel Isoprenoid Modified Proteins in Halobacteria" Biochem. Biophys. Res. Commum.203. 072-976 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Maki et al.: "Farnesyl Diphoshate Synthase Reaction with Dimethylallyl Diphoshate Analogues Having Oxygen Atoms in Their Chains" Chem. Lett. 389-390 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Sagami et al.: "A Novel Type of Protein Modification by Isoprenoid-derived Materials" J. Biol. Chem.270. 14851-14854 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] A. Koike-Takeshita et al.: "Molecular Cloning and Nucleotide Sequences of the Genes for Two Essential Proteins Constituting a Novel Enzyme System for Heptaprenyl Diphoshate Synthesis" J. Biol. Chem.270. 18396-18400 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Koyama et al.: "Significance of Phe-220 Gin-221 in the Catalytic Mechanism of farnesyl Diphosphate Synthase of Bacillus stearothermophilus" Biochem. Biophys. Res. Commun.212. 681-686 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Maki et al: "Substrate Specificity of Farnesyl Diphosphate Synthase from Bacillus stearothermophilus" Bioorg. Med. Chem. Lett.5. 1605-1608 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Inoue et al.: "Properties of Farnesol Phosphokinase of Botryococcus braunii" Phytochemistry. 40. 377-381 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Sagami et al: "Enzymatic Formation of Dehydrodolichal and Dolichal, New Products Related to Yeast Dolichol Biosynthesis" J. Biol. Chem. 271. 9560-9566 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Koyama et al.: "Identification of Significant Residues in the Substrate Binding Site of Farnesyl Diphosphate Synthase" Biochemistry. 35. 9533-9538 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S. Ohnuma et al.: "Conversion from Farnesyl Diphoshate Synthase to Geranylgeranyl Diphoshate Synthase by Random Chamical Mutagenesis" J. Biol. Chem.271. 10087-10095 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A. Kurisaki et al.: "Proteolytic Release of Dehydrodolichyl Diphoshate Synthase from Pig Testis Microsome" Biosci. Biotech. Biochem.60. 1109-1114 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Koyama et al.: "Isopentenyl Diphosphate Isomerase in Rubber Latex" Phytochemistry. 43. 769-772 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A. Kurisaki et al.: "Distribution of Polyprenols and Dolichols in Soybean Plant" Phytochemistry. 44. 45-50 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] A. Koike-Takeshita et al.: "Identification of Novel Gene Cluster Participating in Menaquinone (Vitamin K_2) Biosynthesis. Clorung and Sequence Determination of the 2-Heptaprenyl-1, 4-naphthoquinone Methyltransferase Gene of Bacillus stearothermophilus" J. Biol. Chem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Zhan et al.: "Two Cistrons of the gerC Operon of Bacillus subtilis Encode the Two Subunits Heptaprenyl Diphoshate Symthase" J. Bacteriol.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Ohkura et al.: "A Partial Defeciency of Dehydrodolichol Reduction is a Cause Carbohydrate-Defecient Glycoprotein Syndrome Type I"

    • Related Report
      1996 Annual Research Report
  • [Publications] J. Biol. Chem. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐上 博、小倉協三: "プレニル化蛋白質の発見とイソプレノイド生化学の新展開" 生化学, 14 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] 小倉協三: "酵素研究の新展開(一島英治他編):イソプレノイド成合成の分子機構" 東京化学同人, 12 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 小倉協三: "有機化学のフロンティア(日本化学研究会編):イソプレノイドの成合成" 培風館, 22 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 小倉協三: "酵素による炭素・炭素結合形成反応と有機合成への応用" 有機合成化学会誌, 11 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K. Ogura, T. Koyama: "Mechanistic Enzymology and Molecular Genetics of Chain Elongation in Isoprenoid Biosynthesis" Kodansya Scientific, 23 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K. Ogura: "Polyprenyl Diphoshate Synthases" Plenum Publishing Corporation (in press),

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Maki et al.: "Farnesyl Diphosphate Synthase Reaction with Dimethylallyl Diphosphate Analogues Having Oxygen Atoms in Their Chains" Chem.Lett.389-390 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Sagami et al.: "A Novel Type of Protein Modification by Isoprenoid-derived Materials" J.Biol.Chem.270. 14851-14854 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Koike-Takeshita et al.: "Molecular Cloning and Nucleotide Sequences of the Genes for Two Essential Proteins Constituting a Novel Enzyme System for Heptaprenyl Diphosphate Synthesis" J.Biol.Chem.270. 18396-18400 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Koyama et al.: "Significance of Phe-220 and Gln-221 in the Catalytic Mechanism of farnesyl Diphosphate Synthase of Bacillus stearothermophilus" Biochem Biophys.Res.Commun.212. 681-686 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Maki et al.: "Substrate Specificity of Farnesyl Diphosphate Synthase from Bacillus stearothermophilus" Bioorg.Med.Chem.Lett.5. 1605-1608 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Inoue et al.: "Properties of Farnesol Phosphokinase of Botryococcus braunii" Phytochemistry. 40. 377-381 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小倉協三: "東京化学同人" 酵素研究の新展開(一島英治他編):イソプレノイド生合成酵素の分子機構, 12 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小倉協三: "培風館" 有機化学の最近の展開(日本化学研究会編):イソプレノイドの生合成化学(印刷中), 総

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Sagami et al.: "Purification and Properties of Geranylgeranyl Diphosphate Synthase from Bovine Brain." J.Biol.Chem.269. 20561-20566 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Inoue et al.: "Phosphoryration of Farnesol by a Cell-free System from Botryococcus Braunii" Biochem.Biophys.Res.Commun.200. 1036-1041 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Inoue et al.: "Formation of Farnesyl Oleate and Three Other Farnesyl Fatty Acid Esters by Cell-free Extracts from Botryococcus Braunii B Race" Phytochemistry. 36. 1203-1207 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Koyama et al.: "Site-directed Mutagenesis of Farnesyl Diphosphate Synthase:Effect of Substitution on the Three Carboxyl Terminal Amino Acids" Can.J.Chem.72. 75-79 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Koyama et al.: "Thermostable Farnesyl Diphosphate Synthase of Bacillus stearothermophilus:Crystallization and Site-directed Mutagenesis" Acta Biochim.Polonica.41. 281-292 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Sagami et al.: "Purification of Geranylgeranyl Diphosphate from Bovine Brain" Acta Biochim.Polonica.41. 293-302 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Maki et al.: "Farnesyl Diphosphate Synthase Reactions of Geranyl Diphosphate Analogues Having Oxygen Atoms in Their Alkyl Chains" Chem.Lett.10. 1841-1844 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Koyama et al.: "Structural and Functional Roles of the Cysteine Residues of Bacillus stearothermophilus Farnesyl Diphosphate Synthase" Biochemistry. 33. 12644-12648 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Sagami et al.: "Novel Isoprenoid Modified Proteins in Halobacteria" Biochem.Biophys.Res.Commun.203. 972-976 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Koyama et al.: "Isopentenyl Diphosphate Isomerase in Rubber Latex" Phytochemistry. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Asawatreratanakul et al.: "Characterization of Hevea Isopentenyl Diphosphate Isomerase" Proceedings of the 11th FAOBMB Symposium. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Koyama et al.: "Analysis of Prenyltransferase Reaction Products from C-Serum of Hevea brasiliensis" Proceedings of the 11th FAOBMB Symposium. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Inoue et al.: "Properties of Farnesol Phosphokinase of Botryococcus braunii" Phytochemistry. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐上博 他: "プレニル化蛋白質の発見とイソプレノイド生化学の新展開" 生化学. 12. 1488-1501 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小倉協三: "酵素研究の新展開(-島英治他編):イソプレノイド生化学の新展開" 東京化学同人(印刷予定),

    • Related Report
      1994 Annual Research Report
  • [Publications] 小倉協三: "有機化学の最近の展開(日本化学研究会編):イソプレノイドの生合成化学" 培風館(印刷予定),

    • Related Report
      1994 Annual Research Report
  • [Publications] 古山種俊: "新タンパク質応用工学第11章第4節" フジテクノシステムズ(株)(印刷予定),

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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