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Preparation of Alloproteins Endowed with Artificial Functions

Research Project

Project/Area Number 06403034
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

SISIDO Masahiko  DEPARTMENT OF BIOSCIENCE AND BIOTECHNOLOGY FACULTY OF ENGINEERING,OKAYAMA UNIVERSITY PROFESSOR, 工学部, 教授 (60026268)

Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥26,300,000 (Direct Cost: ¥26,300,000)
Fiscal Year 1996: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1995: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1994: ¥20,000,000 (Direct Cost: ¥20,000,000)
KeywordsAlloproteins / Nonnatural Amino Acids / Extension of Genetic Code / Antibodies / Electron Transfer / たんぱく質 / ストレプトアビジン / エネルギー移動 / フレームシフト / 蛍光スペクトル / 超たんぱく質 / 人工機能 / ストップコドン / ストレプアビジン / 蛍光性アミノ酸 / 核酸塩基アミノ酸
Research Abstract

Proteins containing nonnatural amino acids at specific sites (alloproteins) were preparaed by the extension of gentic engineering technique. The alloproteins were endowed with artificial functions by the incorporation of nonnatural amino acids carrying specialty side groups. The results are summarized as follows.
(1)Extension of Amino Acids : A variety of nonnatural amino acids carrying specialty side groups were newly synthesized and tested for the adaptability to in vitro E.Coli ribosomal system. Those that carry linear aromatic groups were found to be accepted by the ribosomal system. The results opened a way for the photo-and electro-functionalization of proteins.
(2)Extension of Genetic Code : Codons that may be assigned to nonnatural amino acids were searched. Some 4-base codons, such as CGGG and AGGU were found to be effective for this purpose. The 4-base codons may be used for multiple incorporation of different nonnatural amino acids into single proteins
(3)Use of Monoclonal Antibodies as the Controlling Factor of Alloproteins : Monoclonal antibodies aganinst nonnatural amino acids were prepared. Those were used to photo-and electro-contorl of alloproteins that carry azobenzene groups or anthraquinonyl groups.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] M.Imaizumi et al: "Fluorescence Behavior of Pyrenyl Groups Bound to Anti-Pyrenyl Antibody" Journal of Physical Chemistry. 99・11. 3810-3814 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kawashima, et al: "Photocontrolled Attachment of Anti-Azobenzene Antibody onto Azobenzene-Linked Quartz Surfaces" Chemisty Letters. 1995・7. 491-492 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kuragaki, et al: "Long-Distance Singlet Energy Transfer along α-Helical Polypeptide Chains" Journul of Physical Chemistry. 100・39. 16019-16025 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Hohsaka et al: "Incorporation of Nonnatural Amino Acids into Streptavidmrhrough In vitro Frame-Shift Suppression" Journal of America Chemical Society. 118・40. 9778-9779 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido, et al: "Photoinduced Electron Transfers on α-Helical Polypeptides" Peptide Chemistry. 1995. 513-516 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kuragaki, et al: "Highly Efficient Energy Transfer from Tryptophans in Avidin Tetramer to Biotin-Linked Pyrenylalanine" Bulletin of Chemical Society,Japan. 70・1. 261-265 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kira, et al: "Synthesis and Redox Property of Polypeptides Containing L-Ferrocenylalanine" Chemistry Letters. 1997・1. 89-90 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido, et al: "Design and Synthesis of Peptides and Polypeptides of Electron Transporting Functions" Peptide Chemistry. 1996. 473-476 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Matsubara, et al: "Synthesis and Conformation of Poly (L-2-anthra-quinonylalanine)" Macromolecules. 30・9. 2651-2656 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Shinohara, et al: "Electrochemical Properties of Poly(L-2-anthra-quinorylalanine)" Macromalecules. 30・9. 2657-2661 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido, et al: "Photoswitchable Peptide Antigens on Solid Surfaces" Biopolymers. 印刷中.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 宍戸 昌彦 他: "機能性非天然アミノ酸を含む蛋白質の合成と応用" 化学と工業. 49・4. 515-517 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido: "Elementary Photo processes in Designed Chromophore Sequences on α-Helical Polypeptides" Advances in Photochemistry. 22. 197-228 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 宍戸 昌彦: "超蛋白質-生体分子システムの拡張を目指して-" 高分子加工. 46・12. 530-539 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 宍戸 昌彦 他: "新タンパク質応用工学" フシテクノシステム, (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido: "Vesicles" Marcel Dekker Inc, (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 宍戸 昌彦 他: "季刊化学総説No31「超分子を目指す化学」" 学会出版センター, (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Imaizumi, M.Harada and M.Sisido: "Fluorescence Behavior of Pyrenyl Groups Bound to Anti-Pyrenyl Antibody" J.Phys.Chem.99 (11). 3810-3814 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kawashima, M.Sisido, and K.Ichimura: "Photocontrolled Attachment of Anti-Azobenzene Antibody onto Azobenzene-Linked Quartz Surfaces" Chem.Lett.1995 (7). 491-492 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kuragaki and M.Sisido: "Long-Distance Singlet Energy Transfer along alpha-Helical Polypeptide Chains" J.Phys.Chem.100 (39). 16019-16025 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Hohsaka, Y.Ashizuka, H.Murakami, and M.Sisido: "Incorporation of Nonnatural Amino Acids into Streptavidin through In Vitro Frame-Shift Suppression" J.Am.Chem.Soc.118 (40). 9778-9779 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido and M.Kuragaki: "Photoinduced Electron Transfers on alpha-Helical Polypeptides" Peptide Chem. 1995. 513-516 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kuragaki and M.Sisido: "Highly Efficient Energy Transfer from Tryptophans in Avidin Tetramer to Biotin-Linked Pyrenylalanine" Bull.Chem.Soc.Jpn.70 (1). 261-265 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kira, T.Matsubara, H.Shinohara, and M.Sisido: "Synthesis and Redox Property of Polypeptides Containing L-Ferrocenylalanine" Chem.Lett.1997 (1). 89-90 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido, T.Matsubara, and H.Shinohara: "Design and Synthesis of Peptides and Polypeptides of Electron Transporting Functions" Peptide Chem.1996. 473-476 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Matsubara, H.Shinohara, and M.Sisido: "Synthesis and Conformation of Poly (L-2-anthraquinonylalanine)" Macromolecules. 30 (9). 2651-2656 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Shinohara, T.Matsubara, and M.Sisido: "Electrochemical Properties of Poly (L-2-anthraquinonylalanine)" Macromolecules. 30 (9). 2657-2661 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido, M.Harada, K.Kawashima, H.Ebato, and Y.Okahata: "Photoswitchable Peptide Antigens on Solid Surfaces" Biopolymers. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido: "Elementary Photoprocesses in Designed Chromophore Sequences on alpha-Helical Polypeptides" Adv.Photochem.22. 197-228 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido: Photoenergy Harvesting on Two-Dimensional Vesicular Assemblies in "Vesicles", M.Rosoff, ed.Chapter 14. Marcel Dekker, Inc., 563-592 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sisido and M.Kuragaki: "Photoinduced Electron Transfer on α-Helical Polypeptides" Peptide Chemistry. 1995. 513-516 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kuragaki and M.Sisido: "Long Distance Singlet Energy Transfer along α-Helical Polypeptide Chains" Journal of Physical Chemistry. 100(39). 16019-16025 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Hohsaka,Y.Ashizuka,H.Murakami,and M.Sisido: "Incorporation of Nonnatural Amino Acide into Streptavidin through In Vitro Frame-Shift Suppression" Journal of American Chemical Siciety. 118(40). 9778-9779 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kuragaki and M.Sisido: "Highly Efficient Energy Transfer from Tryptophans in Avidin Tetramer to Biotin-Linked Pyrenylalanine" Bulletin of Chemical Society,Japan. 70(1). 261-265 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kira,T.Matsubara,H.Shinohara,and M.Sisido: "Synthesis and Redox Property of Polypeptides Containing L-Ferrocenylalanine" Chemistry Letters. 1997(1). 89-90 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 宍戸昌彦,芳坂貴弘: "機能性非天然アミノ酸を含む蛋白質の合成と応用" 化学と工業. 49(4). 515-517 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Sisido: ""Elementary Photoprocesses in Designed Chromophore Sequence on α-Helical Polypeptides" in Adv.Photochem." John Wiley & Sons,Ine (印刷中), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Imaizumi,M.Harada,and M.Sisido: "Fluorescence Behavior of Pyrenyl Group Bound to Anti-Pyrenyl Antibody" Journal of Physical Chemistry. 99(11). 3810-3814 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Narisawa,R.Kishi,and M.Sisido: "Photocontral of Orientation of Photochromic Dichroic Dyes in Cholesteric Polymer Films" Macromol.Chem.Phys.,. 196. 1419-1430 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Kawashima,M.Shisido,and K.Ichimura: "Photocontrolled Attachment of Antl-Azobenzene Antibody onto Azobenzene-Coated Quartz Surfaces" Chem.Lett.,. 1995.

    • Related Report
      1995 Annual Research Report
  • [Publications] 491-492 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Sisido: "Photoenergy Harvesting on 2-Dimensional Vesicular Assemblies in “Vesicles"" Marcel Dekker Inc.USA(印刷中),

    • Related Report
      1995 Annual Research Report
  • [Publications] 宍戸昌彦: "超たんぱく質……非天然アミノ酸を含む、人工機能たんぱく質……季刊化学総説「超分子をめざす化学」" 日本化学会編(印刷中),

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Imaizumi: "Preparotion of Anti-Pyrenyl Antibody and Its Chiral Recognition against a Nonnatural Amino Acid Carrying Pyrenyl Group" Chemistry Letters. 1994. 51-52 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Hohsaka: "Photoswitching of NAD^+-Mediated Enzyme Reaction through Photoreversible Antigen-Antibody Reaction" Journal of the American Chemical Society. 116. 413-414 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Hohsaka: "Site-Specific Incorporation of Photofunctional Nonnatural Amino Acids into a Polypeptide through in vitro Protein Biosynthesis" FEBS Letters. 344. 171-174 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Harada: "Photocontrolled Uptake and Release of Photochromic Heptens by Monoclonal Antibodies" Bulletin of the Chemical Society,Japan. 67. 1380-1385 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Imaizumi: "Fluorescence Behavior of Pyrenyl Groups bound to Anti-Pyrenyl Antibody" Journal of Physical Chemistry. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Narisawa: "Die Makromolecular Chemie" Photocontrol of Orientation of Photochromic Dichroic Dyes in Cholesteric Polymer Films, ((印刷中))

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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