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DEVELOPMENT OF SUPER-BIOARTIFICIAL LIVER BASED ON THE HEPATOCYTE-SPECIFIC POLYMER DESIGN AND GENETIC ENGINEERING

Research Project

Project/Area Number 06403035
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionTOKYO INSTITUTE OF TECHNOLOGY

Principal Investigator

AKAIKE Toshihiro  TOKYO INSTITUTION OF TECHNOLOGY Faculty of Bioscience and Technology, Professor, 生命理工学部, 教授 (30101207)

Co-Investigator(Kenkyū-buntansha) WATANABE Yoshifumi  TOKYO INSTITUTE OF TECHNOLOGY,Faculuty of Bioscience and Technology, Assistant, 生命理工学部, 助手 (40231013)
MUTOH Yasutoshi  Gifu University, School of Medicine Professor, 医学部, 教授 (20010069)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 1996: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1995: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1994: ¥7,500,000 (Direct Cost: ¥7,500,000)
KeywordsSuper-bioartificial liver / asialoglycoprotein model / super-hepatocyte / gene transfection / biomimetics / hepatocyte / extracellular matrix / スーパー人工肝臓 / アシアロ糖タンパク質モデル / 細胞増殖性 / 細胞移動性 / 高分子ミセル / ハイブリッド人工肝臓 / β-ガラクトース / バイオ人工肝臓 / Fアクチン / PVLA / 細胞移動 / リポフェクション法
Research Abstract

This research project aimed at the development of super-bioartificial liver which is based on the cell biology, genetic engineering and polymer chemistry. The main approaches are as follows :
1 Design and syntheses of asialoglycoprotein model polymer(PVLA)were successfully made to regulate the cell attachment, morphology, differentiation and proliferation of hepatocytes. The clarification of the machanism regulating the cellular phonomena was made based on the AFM and QCM analysis of polymer coated surface.
2 Cell-specific gene-transfection was successfully made to hepatocytes cultured on PVLA with use of Lipofection method.
3 Development of hepatocyte proliferation by control of cyrokine network of TNF-alpha was made for bioartificial liver.
4 Clarification of the development of Liver cells community was done focused on the analysis of terminal differentiation of Kupffer cells from bone marrow cells

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Yoshifumi WATANABE, Masahiko MORITA and Toshihiro AKAIKE: "Concanavalin A Induces Perforin-Mediated But Not Fas-Mediated Hepatic Injury." Hepatology. (24)「3」. 702-710 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 大河原 久子, 宮崎 純一, 苅部 幸代, 佐藤 純彦, 寺岡 慧, 中川 芳彦, 広谷 紗千子, 太田 和夫, 赤池 敏宏: "Diffusion chamber型人工膵島における免疫隔離膜としての高分子膜の孔径の評価" 人工臓器. (25)「4」. 794-797 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.OHGAWARA, J.MIYAZAKI, S.KARIBE, N.KATAGIRI, F.TASHIRO, T.AKAIKE: "Assessment of pore size of a semipermeable membrane for immunoisolation on xenoimplantation of pancreatic B cell using a diffusion chamber." Transplantation proceedings.(27)「6」. 3319-3320 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.OHGAWARA, J.MIYAZAKI, Y.NAKAGAWA, S.SATO, S.KARIBE, T.AKAIKE: "Xenoimplantation using a diffusion chamber with a B-cell line (MN6) as a bioartificial endocrine pancreas (Bio-AEP)." Cell transplantation.(5)「5S-1」. S71-S73 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.S.Cho, Y.Lleong, T.Ishihara, R.Takei, J.U.Park, K.H.Park, A.Maruyama, T.Akaike: "Simple preparation of nanoparticles coated with carbohydrate-carrying polymers." Biomaterials.18(4). 323-326 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 赤池 敏宏, 武居 良太郎, 朴 根弘: "細胞工学とME-細胞認識の制御によるハイブリット人工臓器・Cell Delivaryをめざして" MAXELL BME. 2,410. (In Press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.CHO, A.KOBAYASHI, M.GOTO, K.PARK, T.AKAIKE: "Difference in Adhesion and Proliferation of Fibroblast Between Langmuir-Blodgett Films and Cast Surfaces of Poly (γ-benzy) L-glutamate/Poly (enthylene oxide) diblock copolymer." J.Biomedical Mater. res. 32. 425-432 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshifumi WATANABE,Masahiko MORITA,Toshihiro AKAIKE: "Concanavalin A Induces Perforin-Mediated But Not Fas-Mediated Hapatic Injury." Hepatology. (24)[3]. 702-710 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.OHGAWARA,J.MIYAZAKI,S.KARIBE,N.KATAGIRI,F.TASHIRO,T.AKAIKE: "Assessment of pore size of a semipermeable membrane for Immunoisolation on xenoimplantation of Pancreatic B cell using a diffusion chamber." Transplantation proceedings.(27)[6]. 3319-3320 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.OHGAWARA,J.MIYAZAKI,Y.NAKAGAWA,S.SATO,S.KARIBE,T.AKAIKE: "Xenoimplantation using a diffusion chamber with a B-cell line(MIN6) as a bioartificial endocrine pancreas (Bio-AEP)" Cell transplantation. (5)[5S-1]. S71-S73 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.S.Cho, Y.I.Jeong, T.Ishihara, R.Takei, J.U.Park, K.H.Park, A.Maruyama, T.Araike: "Simple preparation of nanoparticles coated with carbohydratecarrying polymers." Biomaterials. (18)[4]. 323-326 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.S.Cho, Y.I.Jeong, T.Ishihara, R.Takei, J.U.Park, K.H.Park, A.Maruyama, T.Araike: "Simple preparation of nanoparticles coated with carbohydratecarrying polymers." Biomaterials. (18)[4]. 323-326 (1996)C.CHO,A.KOBAYASHI,M.GOTO,K.PARK,T.TAKAIKE:

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] "Difference in Adhesion and Proliferation of Firoblast Between Langmuir-Blodgett Films and Cast Surfaces of Poly (gamma-benzyl L-glutamate/Poly(enthylene oxide) diblock copolymer." J.Biomedical Mater.res.(32). 425-432 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshifumi WATANABE,Masahiko MORITA and Toshihiro AKAIKE: "Concanavalin A Induces Perfonin-Mediated But Not Fas-Mediated Hepatic Injury" Hepatology. (24)「3」. 702-710 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大河原久子.宮崎純一.苅部幸代.佐藤純彦.寺岡 慧.中川芳彦.広谷紗千子.太田和夫.赤池敏宏: "Diffusion chamber型人工膵島 における免疫隔離膜としての高分子膜の孔径の評価" 人工臓器. (25)「4」. 794-797 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.OHGAWARA,J.MIYAZAKI,S.KARIBE,N.KATAGIRI F.TASHIRO,T.AKAIKE: "Assessment of pore size of a semipemeable membrane for immunoisolation on xenoimplantation of pancreatic B cell using a diffusion chamber." Transplantation proceedings.(27)「6」. 3319-3320 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.OHGAWARA,J.MIYAZAKI,Y.NAKAGAWA,S.SATO S.KARIBE,T.AKAIKE.: "Xenoimplantation using a diffusion chamber with a B-cell line (MIN6)as a bioartificial endocrine pancreas(Bio-AEP)." Cell transplantation. (5)「5S-1」. S71-S73 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] C.S.Cho,Y.Lleong.T.Ishihara,R.Takei,J.U.Park K.H.Park,A.Maruyama,T.Akaike.: "Simple preparation of nanoparticles coated with carbohydrate-canying polymers." Biomaterials.18(4). 323-326 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 赤池 敏宏、武居 良太郎.朴 根弘: "細胞工学とME-細胞認識の制御によるハイブリッド人工臓器・Cell Delivaryをめざして" MAXELL BME. 2,410(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] C.CHO.A.KOBAYASHI,M.GOTO.K.PARK,T.AKAIKE.: "Difference in Adhesion and Proliferation of Fibroblast Between Langmuir-Blodgett Films and Cast Surfaces of Poly(γ-benzy1 L-glutamate/Poly(enthylene oxide)diblock copolymer." J.Biomedical Mater.res.32. 425-432 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masahiko MORITA, Yoshifumi WATANABE,Toshihiro AKAIKE: "Protective Effect of Hepatocytes Growth Factor(HGF)on Interferon-γ(IFN-γ) Induced Cytotoxicity in Mouse Hepatocytes" HEPATOLOGY. (21)[5]. 1585-1593 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kouei SHINZAWA, Yoshifumi WATANABE, Toshihiro AKAIKE: "Primary cultured munine hepatocyte but not hepatoma cells regulate the cell number through density-dependent cell death" Cell Death and Differentiation,. (2). 133-140 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chong-su CHO, Akira KOBAYASHI, Mitsuaki GOTO, Keung-Hong PARK, Toshihiro AKAIKE: "Difference in Adhesion and Proliferation of Fibroblast between Langmuir-Blodgett Films and Cast Surfaces of Poly(g-benzy 1 L-glutamate)/Poly(enthylene oxide)diblock copolymer" J. Biomedical Mater. Res.(印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Chong-Su CHO, Mitsuaki GOTO, Akira KOBAYASHI, Kazuhiko KOBAYASHI Toshihiro AKAIKE: "Effect of ligand orientation on hepatocyte attachment onto the poly(N-P-vinylbenzyl-o-β-D-galactopyranosyl-D-gluconamide) as a model ligand of asialogglycoprotein" J. Biomater. Sci. Polym. Edn.(印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] 赤池 敏宏、丸山 厚、由良 洋文、後藤 光昭: "インテリジェントマテリアル" DDSの進歩 1995-96. 12-22 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chong-Su CHO, Mitsuaki GOTO, Akira KOBAYASHI, Kazuhiko KOBAYASHI Toshihiro AKAIKE: "Effect of ligand orientation on hepatocyte attachment onto the poly (N-p-vinylbenzyl-o-β-D-guluconamide) as amodel ligand of asialoglicoprotein" J. biomater. Sci. Polym. edn.(印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Toshihiro AKAIKE, et al: "Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems" Springer, 381 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.KOBAYASHI et al: "Receptormediated Regulation of Differentiation and Proliferation of Hepatocyte by Synthetic Polymer Model of Asaloglycoproteon" J.Biomater.Sci.Polymer Edn,. 6. 325-342 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.MATSUSHITA et al: "Induction of Cytochrome P-450s and Expression of Liver-specific Genes in Rat Primary Hepatocytes Cultured on Different Extracellular Matrices" Biosci.Biotech.Biochem.58. 1514-1516 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.MORITA et al: "Infammatory cytokines upregulate the ICAM-1 expression on primary cultured mouse hepatocytes and T cell adhesion" Hepatology. 19. 426-431 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.WATANABE et al: "Highly Efficient Transfection into Primary Cultured Mouse Hepatocytes by Use of Cation-Liposomes : An Application for Immunization" J.Biochem.116. 1220-1226 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.WATANABE and T.AKAIKE: "Activation Signal Induces the Expression of B Cell-Specific CD45R Epitope(6B2) on Murine T Cells" Scand.J.Immunol.39. 419-425 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 後藤光昭,赤池敏宏: "人工臓器1994-1995" 中山書店, 312 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 赤池敏宏,後藤光昭,小林明,小林一清: "糖鎖と細胞" 日経サイエンス, 151 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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