• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanisms of branch migration and resolution of Holliday junctions

Research Project

Project/Area Number 06404002
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionOsaka University

Principal Investigator

SHINAGAWA Hideo  Res.Inst.Microbial Diseases, Osaka Univ.Professor, 微生物病研究所, 教授 (40029799)

Co-Investigator(Kenkyū-buntansha) IWASAKI Hiroshi  Res.Inst.Microbial Diseases, Osaka Univ.Research Associate, 微生物病研究所, 助手 (60232659)
雨村 光子  大阪大学, 微生物病研究所, 教務職員 (80159467)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥24,600,000 (Direct Cost: ¥24,600,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1995: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1994: ¥14,800,000 (Direct Cost: ¥14,800,000)
KeywordsDNA recombination / DNA repair / Holliday junction / Mutant / RecG protein / RuvA protein / RuvB protein / RuvC protein / RuvCエンドヌクレアーゼ / RuvGタンパク質 / RuvCタンパク / エンドヌクレアーゼ / X線結晶回折 / 染色体
Research Abstract

We studied reaction mechanisms and functions of RuvA and RuvB protein complex and RecG protein which drives branch migration of Holliday junctions and RuvC protein which endonucleolytically resolves Holliday junctions.
1.We discovered that RecG protein has a unique function to resolve the R-loops formed at the replication origin and negatively regulate the initiation of replication.
2.We studied the substrate specificity of RuvC resolvase with various synthetic Holliday junctions and found that the existence of thymine residue at or near crossover point is important but the sequence homology at the junction is not important for the cleavage.
3.We cloned and analyzed the ruvA,ruvB and ruvC genes of Pseudomonas aeruginosa and found that although the arrangement of the genes are different from that of the Escherichia coli genes, they possess the similar functions.
4.We cloned, expressed the Thermus thermophilus ruvB gene and characterized the RuvB protein.
5.We studied the morphology of ruv mutants and recG mutants and found that the abortive recombination in these mutants inhibit the chromosome nondisjunction.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Shinagawa, H. Iwasaki, H.: "Processing the Holliday junction in homologous recombination." Trends Biochem. Sci.21. 107-111 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hishida, T., Iwasaki, H., Ishioka, K. Shinagawa, H.: "Molecular analysis of the Pseudomonas aeryginosa genes, ruvA, ruvB and ruvC, involved in processing of homologous recombination intermediates." Gene. 182. 63-70 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shida, T., Iwasaki, H., Saito, a., Kyogoku, Y. Shinagawa. H.: "Analysis of substrate specificity of the RuvC Holliday junction resolvase with synthetic Holliday junctions." J. Biol. Chem.271. 26105-26109 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shida, T., Iwasaki, H., Shinagawa, H. Kyogoku, Y.: "Characterization and comparison of synthetic immobile and mobile Holliday junctions." J. Biochem.119. 653-658 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuoh, A., Iwasaki, H., Ishioka, K. Shinagawa, H.: "ATP-dependent resolution of R-loops at the ColEl replication origin by Escherichia coli RecG protein, a Holliday junction-specific helicase." EMBOJ.16. 203-209 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinagawa, H.: "SOS response as an adaptive response to DNA damage in prokaryotes. In Stress-inducible Cellular Responses." Base1 : Birkhdddnser Verlag, U. Feige, R. I. Morimoto, I. Yahara and B. Polla, eds., 221-235 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahagi, M., Iwasaki, H.and Shinagawa, H.: "Structural requirements of substrate DNA for binding to and cleavage by RuvC,a Holliday junction resolvase." J.Biol.Chem.269. 15132-15139 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ariyoshi, M., Vassylyev, D.G., Iwasaki, H., Fujishima, A., Shinagawa, H.and Morikawa, K.: "Preliminary crystallographic study of Escherichia coli RuvC protein : an endonuclease specific for Holliday junctions." J.Mol.Biol.241. 281-282 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ariyoshi, M., Vassylyev, D.G., Iwasaki, H., Nakamura, H., Shinagawa, H.and Morikawa, K.: "Atomic structure of the RuvC resolvase : a Holliday junction-specific endonuclease from E.coli." Cell. 78. 1063-1072 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishino, Y., Iwasaki, H., Kato, I.and Shinagawa, H.: "Amino acid sequencemotifs essential to 3'*5' exonuclease activity of Escherichia coli DNA Polymerase II." J.Biol.Chem.269. 14655-14660 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinagawa, H.and Iwasaki, H.: "Molecular mechanisms of Holliday junction processing in Escherichia coli." Adv.Biophys.31. 49-65 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Saito, A., Iwasaki, H., Ariyoshi, M., Morikawa, K.and Shinagawa, H.: "Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase" Proc.Natl.Acad.Sci.USA,92. 7470-7474 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinagawa, H.and Iwasaki, H.: "Processing the Holiday junction in homologous recombination." Trends Biochem.Sci.21. 107-111 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shida, T., Iwasaki, H., Shinagawa, H.and Kyogoku, Y.: "Characterization and comparison of synthetic immobile and mobile Holliday junctions." J.Biochem.119. 653-658 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shida, T., Iwasaki, H., Saito, A., Kyogoku, Y.and Shinagawa, H.: "Analysis of substrate specificity of the RuvC Holliday junction resolvase with synthetic Holliday junctions." J.Biol.Chem.271. 26105-26109 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hishida, T., Iwasaki, H., Ishioka, K.and Shinagawa, H.: "Molecular analysis of the Pseudomonas aeruginosa genes, ruvA,ruvB and ruvC,involved in processing of homologous recombination intermediates." Gene. 182. 63-70 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuoh, A., Iwasaki, H., Ishioka, K.and Shinagawa, H.: "ATP-dependentresolution of R-loops at the ColE1 replication origin by Escherichia coli RecG protein, a Holliday junction-specific helicase." EMBO J.16. 203-209 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinagawa,H.,Iwasaki,H.: "Processing the Holliday junction in homologous recombination." Trends Biochem.Sci.21. 107-111 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hishida,T.,Iwasaki,H.,Ishioka,K.,Shinagawa,H.: "Molecular analysis of the Pseudomonas aerugionosa genes,ruvA,ruvB and ruvC,involved in processing of homologous recombination intermediates." Gene. 182. 63-70 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shida,T.,Iwasaki,H.,Saito,A.,Kyogoku,Y.,Shinagawa,H.: "Analysis of substrate specificity of the RuvC Holliday junction resolvase with synthetic Holiday junctions." J.Biol.Chem.271. 26105-26109 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shida,T.,Iwasaki,H.,Shinagawa,H.,Kyogoku,Y.: "Characterization and comparison of synthetic immobile and mobile Holliday junctions." J.Biochem.119. 653-658 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukuoh,A.,Iwasaki,H.,Ishioka,K.,Shinagawa,H.: "ATP-dependent resolution of R-loops at the ColE1 replication origin by Escherichia coli RecG protein, a Holliday junction-specific helicase." EMBO J.16. 203-209 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shinagawa,H.: "SOS response as an adaptive response to DNA damage in prokaryotes.In Stress-inducible Cellular Responses." Basel:Birkhdddnser Verlag U.Feige,R.I.Morimoto,I.Yahara and B.Polla,eds., 221-235 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saito,A.,Iwasaki,H.,Ariyoshi,M.,Morikawa,K.,Shinagawa,H.: "ldentification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase." Proc.Natl.Acad.Sci.USA. 92. 7470-7474 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shinagawa,H.,Iwasaki,H.: "Molecular mechanisms of Holliday junction processing in Escherichia coli." Adv.Biophys.31. 49-65 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shinagawa,H.,Iwasaki,H.: "Processing the Holliday junction in homologous recombination." Trends in Biochemical Sciences. (in press.). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 品川日出夫: "病気のバイオサイエンス(分担執筆:相同的遺伝子組換え機構)" 大阪大学出版会, 7 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishino,Y.,Iwasaki,H.,Kato,L,Shinagawa,H,: "Amino acid sequence motif essential to 3'→5'exonuclease activity of Escherichia coli DNA polymerase II." J.Biol.OChem.,. 269. 14655-14660 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tahahagi,M.,Iwasaki,H.,Shinagawa,H.: "Structual requirements of substrate DNA for binding to and cleavage by RuvC、 a Holliday junction resolvase.21GC02:J.Biol.Chem.," 269. 15132-15139 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ariyoshi,M.,Vassylyev,D.G.,Fujishima,A.,Iwasaki,H,Shinagawa,H.,Morikawa,K.: "Preliminaly crystallogradphic studv of Excherichia coli RuvC protein:an endonuclease specific for Holliday junctions." J.Mol.Biol.,. 242. 281-282 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ariyoshi,M.,Vassylyev,D.G.,Iwasaki,H.,Nakamura,H.,Shinagawa,H.,Morikawa,K.: "Atomic structure of the RuvC resolvase:a Holliday junction Specific enconuclease from E.coli." Cell. 78. 1063-1072 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 品川日出夫: "組換え修復の遺伝学と酵素学" 細胞工学. 13. 720-731 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 品川日出夫: "臨床分子生物学(分担執筆:DNA修復と突然変異・癌)" 日本臨床, 6 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi