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Morphological Study on the Scavenging Mechanism of Active Oxygen Radicals in Leguminous Crops

Research Project

Project/Area Number 06404006
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 作物学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

SUYAMA Kyouzou (1995)  Tohoku University, Faculty of Agriculture, Professor, 農学部, 教授 (70005635)

星川 清親 (1994)  東北大学, 農学部, 教授 (60011871)

Co-Investigator(Kenkyū-buntansha) IWABUCHI Setuko  Tohoku University, Faculty of Agriculture, 農学部, 助手 (50089794)
OKUBO Kazuyoshi  Tohoku University, Faculty of Agriculture, Professor, 農学部, 教授 (00005612)
須山 享三  東北大学, 農学部, 助教授 (70005635)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥16,800,000 (Direct Cost: ¥16,800,000)
Fiscal Year 1995: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1994: ¥9,900,000 (Direct Cost: ¥9,900,000)
KeywordsChemiluminescence / Soybean / Radical scavenger / Leguminous plant / DDMP saponin / XYZ system / Active oxygen species / 活性酸素 / フリーラジカル / SOD活性 / フラボン / カテキン
Research Abstract

There are many reports on the enzymatic defense of plant kingdom against active oxygen radicals such as SOD (super oxide dismutase), but no report on the non enzymatic mechanism. Also, measurement of active oxygen radicals have been done by electron spin resonance (ESR) and enzymatic methods, but recently we have found photon emission in the presence of hydroperoxide and acetaldehyde.
Oxygen-radical-scavenging characteristics of DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) conjugated saponins in legumenous plants will be the focus of this research. We have isolated genuine saponins, soyasaponins alphag, betag, betaa, gammag and gammaa from soybean, and soyasaponin alphaa from scarlet runner bean. Also soyasaponin betag was isolated from rootstock of the American groundnut. The structure was identified by ^1H-NMR and ^<13>C-NMR and by chemical techniques. A high concentration of DDMP saponin was observed around the cell in fibrovascular bundles connecting the stem to the plumule.
The chemiluminescence (CL) of catechins were measured in the presence of active oxygen species and acetaldehyde (MeCHO). (-) -Epigallocatechin (EGC) exhibited the strongest CL among flavonoids, anthocyanins and catechins. The CL intensity, P,was dependent on the concentrations of active oxygen species, X,such as H_2O_2, catalytic species, Y,such as EGC and receptive species, Z,such as MeCHO as follows ; p=k[X] [Y] [Z] (k ; photon constant). DDMP saponins showed oxygen-radical-scavenging activity assayd by using the xanthin oxidase-NH2OH methods, ESR and chemiluminescence. One milligram of DDMP saponin/mg exhibited superoxide-scavenging activity of a degree equvalent to 17.1 units of superoxide dismutase/ml measured by the ESR spin trappin method. We have found that group A and DDMP saponins are Y equivalent to EGC and Z equivalent to MeCHO,respectively.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (84 results)

All Other

All Publications (84 results)

  • [Publications] Y. Yoshiki et al.: "Saponin conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Phaseolus coccineus" Phytochemistry. 36. 1009-1012 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Okubo et al.: "DDMP-conjugated saponins isolated from groundnut (Apios Americana)" Biosci. Biotech. Biochem.58. 2248-2250 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J. H. Kim et al.: "Relationships among various saponin types in soybean (Glycine max)" Korean Soybean Digest. 11. 55-61 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大久保一良等: "大豆加工食品の風味(香りと色)に及ぼすDDMPサポニンの影響" 大豆たん白質研究会会誌. 15. 36-40 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Kanazawa et al.: "Plasma peroxidized low-densith lipoprotein with hydroperoxidized cholesteryl linoleate estimated in patients with familial hypercholesterolemia" Pathobiogy. 62. 269-282 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Yoshiki et al.: "Saponin conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Dolichos lablab the SOD-like activity" Phytochemistry. 38. 229-231 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Kanazawa et al.: "Protective effects of soy protein on the peroxidizability of lipoproteins in cerebrovascular deseases" J. Nutrition. 125. 639S-646S (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Yoshiki et al.: "Chemiluminescence of benzoic acid and cinnamic acid, and flavonoids in the presence of aldehyde and hydrogen peroxide or hydroxyl radical by Fenton reaction" Phytochemistry. 39. 225-229 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] C. Tsukamoto et al.: "Factors affecting isoflavone content in soybean seeds : Changes in contents of isoflavones, saponins and composition of fatty acids at different temperatures during seed development" J. Agric. Food Chem.43. 1184-1192 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki and K. Okubo: "Oxygen radical scavenging activity of DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) conjugated saponin in soybean seed" Biosci. Biotech. Biochem.59. 1556-1557 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Chemiluminescense of anthocyanins in the presence of aldehyde and tert-butyl hydroperoxide" J. Biolumin. Chemilumin. 10. 335-338 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.H.Kim et al.: "Genetic studies on saponin content by Vr-Wr graphical analysis in soybean (Glycine max)" Korea Soybean Digest. 12. 74-81 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.H.Kim et al.: "Analysis of GCA and SCA for saponin content in soybean hybrid population" Korea Soybean Digest. 12. 82-88 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Ohtsuki et al.: "A 96-kDa glycyrrhizin-binding protein (gp96) from soybeans acts as a substrate for casein kinase II and it is ighly reated to lipoxygenase 3" J. Biochem.118. 1145-1150 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Yoshikoshi et al.: "Metabolism and nonabsorption of soybean hypocotyl saponins in the rat model" Acta Alimetaria. 24. 355-364 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Shimoyamada et al.: "Partition of siybean saponins between butanol and water" Food Sci. Technol. Int. 1. 18-21 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] R. Amarowicz et al.: "Hemolytic activity of the fractions from the rapeseed ethanolic extract" Fat. Sci. Technol.(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Yoshikoshi et al.: "Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblast cells," Planta med.62,(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Yoshiki et al.: "Chemiluminescence mechanism of catechins in the presense of active oxygen" J. Biolumin. Chemilumin. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Yoshiki et al.: "Chemiluminescence of soybean saponins in the presence of active oxygen species" Plant Science. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Yoshikoshi et al.: "Soybean βg and gallic acid prevent damage of hydrogen peroxide to mouse fibroblast cells" Planta medica. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Okubo: "Health-enhancing ingredients of soybean in food : oxygen radical scavenging characteristics of DDMP saponins and the related substance" Proceedings of Agri-Food Quality 95. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Yoshiki et al.: "Hydrophilic oxygen radical scavengers in leguminous seeds and derived foods" Proceedings of Agri-Food Quality 95. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Yoshiki et al.: "Chemiluminescense of phenolic compounds in the presence of active oxygen species and acetaldehyde" Proceedings of Agri-Food Quality 95. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Okubo and Y. Yoshiki: "Oxygen-radical-scavenging sctivity of DDMP-conjugated saponins and physiological role in leguminous plant" ACS series. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Yoshiki et al.: "Chemiluminescence of oxygen radical scavengers such as DDMP saponins in the presence of radicals and aldehyde" ACS series. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Okubo et al.: "Chemiluminescence of catechins and soybean saponins in the rresence of active oxygen species" ACS series. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Yoshiki et al.: "Reaction of natural radical scavengers with active oxygen species on the photon emisson" ICOFF. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大久保一良: "種子のバイオサイエンス" 学会出版センター, 462 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大久保一良: "植物の世界45号、ダイズ、デイコ、アズキ分担" 朝日新聞社, 2 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 吉城由美子・大久保一良: "がん予防食品の設計、天然ラジカルスカベンジャー分担" シ-・エム・シ-社, 10 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大久保一良・吉城由美子: "医食同源一ダイズサポニンの抗酸化機能分担" 共立出版(印刷中), (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Saponin conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Phaseolus coccineus" Phytochemistry. 36-4. 1009-1012 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Okubo et al.: "K., DDMP-conjugated saponins isolated from groundnut (Apios Americana)" Biosci. Biotech. Biochem.58-12. 2248-2250 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.H.Kim et al.: "Relationships among various saponin types in soybean (Glycine max)" Korean Soybean Digest. 11-2. 55-61 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Kanazawa et al.: "Plasma peroxidized low-density lipoprotein with hydroperoxidized cholesteryl linoleate estimated in patients with familial hypercholesterolemia" Pathobiology. 62. 269-282 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Saponin conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Dolichos lablab and the SOD-like activity" Phytochemistry. 38-1. 229-231 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Kanazawa et al: "Protective effects of soy protein on the peroxidizability of lipoproteins in cerebrovascular deseases" J.Nutrition. 125-3S. 639S-646S (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al: "Chemiluminescence of benzoic acid and cinnamic acid, and flavonoids in the presence of aldehyde and hydrogen peroxide or hydroxyl radical by Fenton reaction" Phytochemistry. 39-1. 225-229 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] C.Tsukamoto et al.: "Factors affecting isoflavone content in soybean seeds : Changes in contents of isoflavones, saponins and composition of fatty acids at different temperatures during seed development" J.Agric. Food Chem. 43-5. 1184-1192 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Oxygen radical scavenging activity of DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) conjugated saponin in soybean seed" Biosci. Biotech. Biochem.59-8. 1556-1557 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Chemiluminescence of anthocyanins in the presence of aldehyde and tert-butyl hydroperoxide" J.Biolumin. Chemilumin.10. 335-338 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.H.Kim et al.: "Genetic studies on saponin content by Vr-Wr graphical analysis in soybean (Glycine max)" Korea Soybean Digest. 12-2. 74-81 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.H.Kim et al.: "Analysis of GCA and SCA for saponin content in soybean hybrid population" Korea Soybean Digest. 12-2. 82-88 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Ohtsuki et al.: "A 96-kDa glycyrrhizin-binding protein (gp96) from soybeans acts as a substrate for casein kinase II and it is highly related to lipoxygenase 3." J.Biochem.118-6. 1145-1150 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Yoshikoshi et al.: "Metabolism and nonabsorption of soybean hypocotyl saponins in the rat model" Acta Alimetaria. 24-4. 355-364 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Shimoyamada et al.: "Partition of siybean saponins between butanol and water" Food Sci. Technol. Int.1-2. 18-21 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] R.Amarowicz et al.: "Hemolytic activity of the fractions from the rapeseed ethanolic extract" Fat Sci. Technol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Yoshikoshi et al.: "Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblast cells" Planta med.62. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Yoshiki et al.: "Chemiluminescence mechanism of catechins in the prsence of active oxygen" J.Biolumin. Chemilumin.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Yoshiki et al.: "Chemiluminescence of soybean saponins in the presence of active oxygen species" Plant Science. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Yoshikoshi et al.: "Soybean betag and gallic acid prevent damage of hydrogen peroxide to mouse fibroblast cells" Planta medica. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Okubo: "Health-enhancing ingredients of soybean in food : oxygen radical scavenging characteristics of DDMP saponins and the related substances" Proceedings of Agri-Food Quality 95. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Hydrophilic oxygen radical scavengers in leguminous seeds and derived foods" Proceedings of Agri-Food Quality 95. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Chemiluminescence of phenolic compounds in the presence of active oxygen species and acetaldehyde" Proceedings of Agri-Food Quality 95. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Okubo and Y.Yoshiki: "Oxygen-radical-scavenging activity of DDMP-conjugated saponins and physiological role in leguminous plant" ACS series, Saponins Used Traditional and Modern Medicine or Saponins used in Food and Agriculture, edited by George R.Waller and Kazuo Yamasaki, published by Plenum Publishing Co., New York, NY. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Chemiluminescence of oxygen radical scavengers such as DDMP saponins in the presence of radicals and aldehyde" ACS series, Saponins Used Traditional and Modern Medicine or Saponins used in Food and Agriculture, edited by George R.Waller and Kazuo Yamasaki, published by Plenum Publishing Co., New York, NY. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Okubo et al.: "Chemiluminescence of catechins and soybean saponins in the presence of active oxygen species" ACS series, edited by Shahidi. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Yoshiki et al.: "Saponin conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Dolichos lablab and the SOD-like activity." Phytochemistry. 38. 229-231 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Kanazawa et al.: "Protective effects of soy protein on the peroxidizability of lipoproteins in cerebrovascular deseases" J.Nutrition. 125. 639S-646S (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Yoshiki et al.: "Chemiluminescence of benzoic acid and cinnamic acid,and flavonoids in the presence of aldehyde and hydrogen peroxide or hydroxyl radical by Fenton reaction" Phytochemistry. 39. 225-229 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] C.Tsukamoto et al.: "Factors affecting isoflavone content in soybean seeds:Changes in contents of isoflavones,saponins and composition of fatty acids at different temperatures during seed development," J.Agric.Food Chem.,. 43. 1184-1192 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Yoshiki and K.Okubo: "Oxygen radical scavenging activity of DDMP(2,3-dihydro-2,5-dihydroxy-6-methly-4H-pyran-4-one)conjugated saponin in soybean seed." Biosci.Biotech.Biochem.,. 59. 1556-1557 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Yoshiki et al.: "Chemiluminescence of anthocyanins in the presence of aldehyde and tert-butyl hydroperoxide" J.Biolumin.Chemilumin.10. 335-338 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J.H.Kim et al.: "Genetic studies on saponin content by Vr-Wr graphical analysis in soybean(Glycine max)" Korea Soybean Digest. 12. 74-81 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J.H.Kim et al.: "Analysis of GCA and SCA for saponin content in soybean hybrid population" Korea Soybean Digest. 12. 82-88 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Ohtsuki et al.: "A96-kDa glycyrrhizin-binding protein(gp96)from soybeans acts as a substrate for casein kinase II and it is highly related to lipoxygenase 3." J.Biochem.,. 118. 1145-1150 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshikoshi et al.: "Metabolism and nonabsorption of soybean hypocotly saponins in the rat model." Acta Alimetaria. 24. 355-364 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Shimoyamada et al.: "Partition of siybean saponins between butanol and water" Food Sci.Technol.lnt.1. 18-21 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] R.Amarowicz et al.: "Hemolytic activity of the fractions from the rapeseed ethanolic extract," Fat Sci.Technol.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshikoshi et al.: "Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblast cells," Planta med.62(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshiki et al.: "Chemiluminescence mechanism of catechins in the presence of active oxygen" J.Biolumin.Chemilumin.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshiki et al.: "Chemiluminescence of soybean saponins in the presence of active oxygen species" Plant Science. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大久保一良: "種子のバイオサイエンス" 学会出版センター, 462 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大久保一良: "植物の世界45号、ダイズ、デイコ、アズキ分担" 朝日新聞社, 2 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大久保一良: "機能性食品の設計-活性酸素消去機能分担" 学会出版センター, 6 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 吉城由美子・大久保一良: "がん予防食品の設計、天然ラジカルスカベンジャー分担" シ-エム・シ-社, 10 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大久保一良・吉城由美子: "医食同源-ダイズサポニンの抗酸化機能分担" 共立出版(印刷中), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Kudou,S.and K.Okubo: "CRC Handbook of Plant and Fungal Toxiccants:lsoflavones" CRC(印刷中), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Yoshiki et al.: "Saponin conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Phaseolus coccineus" Phytochemistry. 36. 1009-1012 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Okubo et al.: "DDMP-conjugated saponins isolated from guoundnut(Apios Americana)" Bioscience Biotenology Biochemistry. 58. 2248-2250 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.H.Kim et al.: "Relationships among various saponin types in soybean(Glycine max)" Korean Soybean Digest. 11. 55-61 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Yoshiki et al.: "Saponin conjugated with 2,3-dihydro-2,5-dihydroxy-6-mithyl-4H-pyran-4-one from from Dolichos lablab and the SOD-like activity." Phytochemistry. 38. 229-231 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大久保一良: "種子のバイオサイエンス" 学会出版センター, 462 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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