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Evidence for Introgression in Japanese Evergreen Azaleas on the Basis of Morphological, Phenological and Genome-Analytical Methods

Research Project

Project/Area Number 06404007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionInstitute of Agriculture and Forestry, University of Tsukuba

Principal Investigator

TAKAYANAGI Kenji  University of Tsukuba, Institute of Agriculture and Forestry, Professor, 農林学系, 教授 (20251019)

Co-Investigator(Kenkyū-buntansha) SUZUKI Tohru  University of Tsukuba, Institute of Agriculture and Forestry.Assistant Professor, 農林学系, 講師 (10272155)
HANDA Takashi  University of Tsukuba, Institute of Agriculture and Forestry.Assistant Professor, 農林学系, 講師 (00192708)
ARISUMI Ken-ichi  Kagoshima University, Faculty of Agriculture, Professor Emeritus, 農学部, 名誉教授 (40035100)
Project Period (FY) 1994 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥27,300,000 (Direct Cost: ¥27,300,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1996: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1995: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1994: ¥14,600,000 (Direct Cost: ¥14,600,000)
KeywordscpDNA / DNA-polymorphism / evergreen azalea / introgression / morphology / PCR-RFLP / ツツジ / PCR / RFLP / RAPD / 形態形質 / PFLP / ミトコンドリア / 葉緑体
Research Abstract

For the purpose of ascending the origin of horticultural evergreen azalea cultivars, we focused on the natural populations of Rhododendron sp.in Kyushu. Two distinct evergreen azaleas native to Kyushu, Rhododenron kiusianum and R.kaempferi are distributed separately, and distinguished by their morphological characters. The former is characterized by a dwarf-plant type, small pink-purple flowers and small elliptical leaves, while the latter has a normal plant-type, big red-orange flowers with red blotches and large oblong leaves. R.kiusianum dwells in the volcanic mountain areas above 1,000m alt., while R.kaempferi grows in lower mountain slopes upto 800m alt. In the zone between 800 to 1000m alt., the natural hybrids of two species are distributed showing the intermediate characters of plant-type, leaf size and shape, and various flower colors.
We applied the PCR-RFLP method for specific region of the genome DNA,and finally PCR-R FLP of chloroplast DNA (cpDNA) at the 16S rDNA region dig … More ested by Hha I,was effective to distinguish R.kiusianum and R.kaempferi. The band patterns ; 1080bp/420bp were specific to R.kiusianum (kiusianum type) and 950bp/420bp/80bp was unique for R.kaempferi (kaempferi type). We first applied this cpDNA marker to the natural population of the Kirishima Mts. Both populations of R.kiusianum and R.kaempferi showed their specific morphology and cpDNAtype. The hybrid populations revealed various types morphologically and ecologically, but there were two-types of cpDNA,kiusianum and kaempferi type. This indicates that the various extents of hybrid nature were determined by nuclear genes, however there are only two types of cytoplasm (chloroplast) which occured due to natural introgression from one species to another in some earlier times.
There are many other populations similar to the Kirishima Mts.of Kyushu, so we surveyed briefly the natural populations of Unzen, Kuju and Aso Mts. Patterns similar to the Kirishima were also found in these populations except Aso Mts.which showed R.kiusianum with kaempferi type cpDNA even at the higher altitude of the mountain.
These findings suggest the origin of the Japanese horticultural evergreen azalea can be traced back to the natural hybrid population of R.kiusianum and R.kaempferi in Kyushu. Further investigations with the DNA markers of nuclear genome should be followed. Less

Report

(5 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] N., Kobayashi・R., Takeuchi・T., Handa and K., Takayanagi: "Cultivar Identification of Evergreen Azalea with RAPD Method." J.Japan.Soc.Hort.Sci.63(4). 611-616 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N., Kobayashi・M., Akabane・T., Handa and K., , Takayanagi: "Inheritance of Morphological Characters and RAPD Markers in Intersubgeneric Hybrids of Azalea,(Rhododendron Kiusianum Makino ×R-indicum(L)Sweet) ×R-Japonicum,(A.Gray)Suringer-F-Flayum Nakai" J.Japan.Soc.Hort.Sci.65(1). 145-153 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N., Kobayashi・S., Kawashima・T., Handa and K., Takayanagi: "Introgression in Japanese evergreen azaleas Rhododendron Kirsionun and R.Kaempferi" Acta Horticulturae. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi, N., R.Takeuchi, T.Handa and K.Takayanagi: "Cultivar identification of evergreen azalea with RAPD method." J.Japan.Soc.Hort.Sci.64. 611-616 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi, N., M.Akabane, T.Handa and K.Takayanagi: "Inheritance of morphological characters and RAPD markers in the subgeneric hybrids of azalea (Rhododendron kiusianum Makino*R,indicum (L.) Sweet) *R.japonicum (A.Gray) Suringer f.flavum Nakai." J.Japan.Soc.Hort.Sci.65. 145-153 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi, N., S.Kawashima, T.Handa, K.Arisumi and K.Takayanagi: "Introgression in Japanese evergreen azaleas (Rhododendron kiusianum and R.kaempferi)." Acta Horticulturae. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi,N.et al.: "Introgression in Japanese evergreen azaleas." Acta Horticulturae. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kobayashi,N.et al.: "A simple and efficient DNA extraction method for plants." Plant Tissue Culture and Biotechnology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kobayashi,N.et al: "Inheritance of morhological characters and RAPD markers in intersubyeneric hybrids of azalen." J.Japan.Soc.Hort.Sci.(園芸学会雑誌). 65. 145-153 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi,N.et al: "Introgression in Japanese evergreen azaleas." Acta Horticulturae. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi, N. et. al.: "Cultivar identification of evergreen azalea with RAPD method." J. Jan. Hort. Sci. (園芸学会雑誌). 64. 611-616 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayashi, N. et. al.: "RAPD markers for morphological determination in hybrid azalea." Plant Genome III. Abstract. 127-127 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayasi,N.et al.: "Cultivar identification and parentage determination of azalea with RAPD markers." 24th International Horticultural Congress. (Abstract). 163-163 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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