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DNA probe synthesize for identification of cry genes from Bacillus thuringiensis which has a broad spectrum in the insecticidal crystal protein.

Research Project

Project/Area Number 06404010
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

IIZUKA Toshihiko  Hokkaido Univ.Fac.of Agri., Prof., 農学部, 教授 (50001441)

Co-Investigator(Kenkyū-buntansha) SAHARA Ken  Hokkaido Univ., Fac.of Agri., Instructor, 農学部, 助手 (30241368)
ASANO Shinichiro  Hokkaido Univ., Fac.of Agri., Instructor, 農学部, 助手 (60222585)
BANDO Hisanori  Hokkaido Univ., Fac.of Agri., As.Prof., 農学部, 助教授 (20189731)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥29,000,000 (Direct Cost: ¥29,000,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 1994: ¥16,100,000 (Direct Cost: ¥16,100,000)
KeywordsBacillus thuringiensis / insecticidal crystal protein (ICP) gene / microbial control of insects / cryV gene / Bacillus Thuringiensis / バチルス・チューリンゲンシス(BT) / 殺虫性結晶蛋白質 / cry遺伝子
Research Abstract

Becillus thuringiensis produces parasporal crystal protein which has insecticidal activity. This protein has been expressed by cry genes from B.thuringiensis. In the research. origonucleotide primers were synthesized depending on each cry gene specific region and DNA probes were prepared by PCR method. According to apply this method. cry genes from B.thuringiensis were easily and quickly isolated and identified.
We have also isolated B.thuringiensis strains from soil samples in Hokkaido and Indonesia. Cry genes from these isolates were identified by using PCR method and a couple of novel cry genes were analyzed for nucleotide sequencing. By the result. cryV gene which has both of Lepidopteracidal and Coleopteracidal activities were found from serovar kurstaki INA-02. These results should be applied for microbial control agents in the near future.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 佐々木潤: "北海道の土壌から分離したBacillus thuringiensis株の性状" 日本蚕糸学雑誌. 63. 361-366 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 浅野眞一郎: "Bacillus thuringiensisのcryIIIA遺伝子の増幅と同定" 日本応用動物昆虫学会誌. 38. 300-302 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐々木潤: "Bacillus thuringiensis分離株の有するcry遺伝子Vの探索" 日本蚕糸学雑誌. 65. 56-61 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, J.: "Insecticidal Activity of the Protein Encoded by the cryV Gene of Bacillus thuringiensis kurstaki INA-02" Current Microbiol.32. 195-200 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 橋本直樹: "cryIA(a)遺伝子プロモーター制御下におけるBacillus thuringiensisICP遺伝子の発現" 日本蚕糸学雑誌. 65. 185-191 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iizuka, T.: "Bacillus thuringiensis Biotechnology and Enbironmental Benefits" Hus Shiang Yuan Publishing, 565 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, J., S.Asano, H.Bando and T.Iizuka: "Characteristics of Bacillus thuringiensis isolated from soil in Hokkaido area of Japan." J.Seric.Sci.Jpn.63. 361-366 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Asano.S., J.Sasaki, H.Bando and T.Iizuka: "Amplification and detection of cryIII gene from Bacillus thuringiensis strains." Jpn.J.Appl.Entoml.Zool.38. 300-302 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, J., S.Asano, H.Bando, B.W.Lay, S.Hastowo and T.Iizuka: "Identification of cryV gene from Bacillus thuringiensis isolates." J.Seric.Sci.Jpn.65. 56-61 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, J., S.Asano, T.Iizuka, H.Bando, B.W.Lay, S.Hastowo, G.Powell and T.Yamamoto: "Insecticidal activity of the protein encoded by the cryV gene of Bacillus thuringiensis kurstaki INA-02" Current Microbiology. 32. 195-200 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hashimoto, N., J.Sasaki.S.Asano.H.Bando and T.Iizuka: "Bacillus thuringiensis ICP gene expression under the control of cryIA (a) gene promoter." J.Seric.Sci.Jpn.65. 185-191 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iizuka, T., J.Sasaki.S.Asano and H.Bando: Comparative studies on isolation identification of Bacillus thuringiensis "Bacillus thuringiensis and Biotechnology and Environment Benefits" Vol.1. Hua Shiang Yuan Publish.Co., 565 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐々木 潤: "Bacillus thuringiensis分離株の有するcry遺伝子Vの探索" 日本蚕糸学雑誌. 65(1). 56-61 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki,J.: "Insecticidal Activity of the Protein Encoded by the cryV Gene of Bacillus thuringiensis kurstaki INA-02" Current Microbiol.32(4). 195-200 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 橋本 直樹: "cryIA(a)遺伝子プロモータ制御下におけるBacillus thuringiensis ICP遺伝子の発現" 日本蚕糸学雑誌. 65(3). 185-191 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐々木潤: "北海道の土壌から分離したBacillus thuringiensis株の性状" 日本蚕糸学雑誌. 63. 361-366 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 浅野真一郎: "Bacillus thuringiensisのcryIIIA遺伝子の増幅と同定" 応用動物昆虫学会誌. 38. 300-302 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] IIZUKA,T.: "Insecticidal activity of the CryIA(a) and CryIB delta-endotoxin of Bacillus thuringiensis was related after the coding region of the gene was truncated and expression in Escherichia coli." J.Sericul.Sci.Jpn. 63. 303-309 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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