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Moleculer genetic study of beta-micoseminoprotein in the prostage gland.

Research Project

Project/Area Number 06404059
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionNagasaki University

Principal Investigator

SAITO Yutaka  Nagasaki University, School of Medicine, Professor, 医学部, 教授 (70039832)

Co-Investigator(Kenkyū-buntansha) SAKAI Hideki  Nagasaki University, School of Medicine Hospital, Assistant, 医学部・附属病院, 助手 (40235122)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥25,200,000 (Direct Cost: ¥25,200,000)
Fiscal Year 1996: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 1994: ¥11,700,000 (Direct Cost: ¥11,700,000)
Keywordsprostate cancer / beta-microseminoprotein / gene mapping / mRNA / in situ hybridization / immunohistochemistry / Prognosis / tumor marker / 遺伝子座 / 突然変異
Research Abstract

1.We assigned the human beta-microseminoprotein (beta-MSP) gene to 10q11.2 with fluorescence in situ hybridization, and the data has shown that the gene is outside the previously identified LOH-regions (10p and 10q24*qter).
2.In mutation analysis by single-strand conformational polymorphism analysis (SSCP), no aberrant bands were detected in 40 cancerous tissue samples analyzed.
3.We analyzed the expression of both beta-MSP mRNA and its protein in biopsy specimens from 104 patients with untreated prostate cancer using both nonradioactive in situ hybridization and immunohistochemistry. Of the 104 specimens, 72 and 96 were negative for beta-MSP mRNA (69.2%) and beta-MSP (92.3%), respectively. Our results showed a lower level of expression of beta-MSP in prostate cancer tissue, compared with benign prostate tissue, and that this phenomenon may be mainly due to the presence of reduced levels of beta-MSP mRNA.
4.To estimate the influence of beta-MSP mRNA expression and three possible prognosti … More c factors, i.e. , patient age, clinical stage and histological grade, on time to progression under endocrine therapy, survival analyzes were performed in Cox's proportional hazards regression model. Multivariate analysis of patients with stage D disease showed that beta-MSP mRNA expression was the only significant prognostic indicator for progression under endocrine therapy (p=0.0034). The presence of cells that express the beta-MSP transcript may be a novel indicator of potentially aggressive prostate cancers.
5.To evaluate the usefulness of beta-MSP as a tumor marker for prostate cancer, we measured bound and free serum beta-MSP levels in 42 patients with non-metastatic prostate cancer preradiotherapy. Tumor stage, grade and free beta-MSP levels were not significant predictors of treatment outcome. Pretreatment PSA and the ratio of bound/free beta-MSP were significant predictors of relapse post radiotherapy on univariate analysis. beta-MSP in addition to PSA may be of prognostic value for patients receiving radiotherapy for non-metastatic prostate cancer. Less

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Hyakutake, H.: "Beta-microseminoprotein immunoreactivity as a new prognostic indicator of prostatic carcinoma." The Prostate. 22. 347-355 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yogi, Y.: "A monoclonal antibody assessment of the beta-microseminoprotein expression in prostatic carcinoma as a prognostic indicator." Acta Medica Nagasakiensia. 40. 13-17 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki, T.: "Assignment of the human beta-microseminoprotein gene(MSMB)chromosome 10q11.2." Cytogenetics and Cell Genetics. 72. 177-178 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 酒井 英樹: "前立腺癌の予後因子" 泌尿器外科. 10. 743-748 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsurusaki, T.: "Cellular expression of beta-microseminoprotein(β-MSP)mRNA and its protein in untreated prostate cancer." The Prostate. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai, H.: "Proceedings of the seventh workshop on prostate cancer" Workshop on Prostate Cancer Organizing Committee, 65 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsurusaki, T.: "Proceedings of the eighth workshop on prostate cancer" Workshop on Prostate Cancer Organizing Committee, 61 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hyakutake H,Sakai H,Yogi Y,Tsuda R,Minami Y,Yushita Y,Kanetake H,Nakazono I,Saito Y: "Beta-microseminoprotein immunoreacitivity as a new prognostic indicator of prostatic carcinoma." Prostate. 22. 347-355 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yogi Y: "A monoclonal antibody assessment of the beta-microseminoprot ein expression in prostatic carcinoma as a prognostic indicator." Acta Med Nagasaki. 40. 13-17 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakaki T,Matsumoto N,Jinno Y,Nisikawa N,Sakai H,Kanetake H,Saito Y: "Assignment of the human beta-microseminoprotein gene (MSMB) to chromosome 10q11.2." Cytogene Cell Gene. 72. 177-178 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai H and Saito Y: "Prognostic factors in prostate cancer." Japanese Journal of Urological Surgery. 10. 743-748 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsurusaki T,Koji T,Sakai H,Kanetake H,Nakane PK,Saito Y: "Celluler expression of beta-microseminoprotein (beta-MSP) mRNA and its protein in untreated prostate cancer." Prostate. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki,T.,et al.: "Assignment of the human β-microseminoprotein gene (MSMB) to chromosome 10q11.2" Cytogenetics and Cell Genetics. 72. 177-178 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yogi,Y.: "A monoclonal antibody assessment of the beta-microsimonoprotein expression in prostatic carcinoma as a prognostic indicator" Acta Medica Nagasakiensia. 40. 13-17 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki,T.,et al.: "Assignment of the human β-microseminoprotein gene (MSMB) to chromosome 10q11.2" Cytogenetics and Cell Genetics.72(in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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