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Mechanisms underlying the alterations of biosignal transduction and cell response in salivary glands

Research Project

Project/Area Number 06404066
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionThe University of Tokushima

Principal Investigator

ISHIDA Hajime  The Univ.of Tokushima, Sch.of Dentistry, Professor, 歯学部, 教授 (70028364)

Co-Investigator(Kenkyū-buntansha) SKOWRONSKI Mariusz  The Univ.of Tokushima, Sch.of Dentistry, Research Associate, 歯学部, 助手 (00294702)
EGUCHI Takafumi  The Univ.of Tokushima, Sch.of Dentistry, Research Associate, 歯学部, 助手 (90263847)
ISHIKAWA Yasuko  The Univ.of Tokushima, Sch.of Dentistry, Associate Professor, 歯学部, 助教授 (40144985)
天野 伊知郎  徳島大学, 歯学部, 助手 (20212566)
Project Period (FY) 1994 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥24,800,000 (Direct Cost: ¥24,800,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1995: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1994: ¥16,500,000 (Direct Cost: ¥16,500,000)
KeywordsSalivary glands / Cell surface receptors / Supersensitivity / Desensitization / GTP binding proteins / Phosphorylation / Dephosphorylation / Okadaic acid / 40KDa蛋白質 / 34KDa蛋白質 / 老化 / 細胞増殖 / イソプロテレノール / 核蛋白質 / ヒストン蛋白質 / 非ヒストン蛋白質 / 唾液分泌 / 感作 / 脱感作 / 受容体 / G蛋白質
Research Abstract

The functional alterations of cell surface receptors cause the changes of G protein functions and of cellular responses. In this study, we investigated the mechansims underlying the alterations of cell responses of salivary glands induced by beta agonists, acetylcholine or histamine, and the age-dependent changes in the cell proliferation of the glands of rats treated with beta agonists, and obtained the results as follows.
(1) Treatment for 30min of saliary glands with isoproterenol (IPR) or histamine resulted in the desensitization of salivary secretion in response to the agonist. The treatment for less than 10min with IPR caused conversely the supersensitivity of the secretion, but that with histamine did not.
(2) The enhancement and the suppresion of Gi protein functions were coupled with the desensitization and the supersensitivity induced by IPR,respectively. But the changes in Gs protein functions were not observed in the tissues caused the desensitization or the supersensitivity. Immunoblot analyzes with AS/7 and RM/1 demonstrated that the levels of Gi2alpha and Gsalpha proteins were not modified by beta_2 agonist- or histamine- treatment.
(3) The decrease and the increase in the phosphorylation of Gi2alpha protein coupled with the desensitization and the supersensitivity of amylase secretion by IPR,respectively. Pretreatment of the tissues with okadaic acid at the concentrations to inhibit protein phosphatase2A activity completely blocked the enhancement of Gi2alpha protein function and resulted in the disappearance of the desensitization or in the enhancement of the supersensitivity.
(4) The changes in the secretory responses of the glands to beta_2 agonists or acetylcholine after birth paralleled with those in the neurotransmitter receptor and in the function of G proteins.
(5) The time required to initiate DNA synthesis in the glands after IPR administration increased significantly with age and the level of DNA synthesis decreased markedly till 16 weeks old.

Report

(5 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] YasukoIshikawa: "Mechanism of isoproterenol-induced heterologous desensitization of mucin secretion from rat submendibular glands." Biochimica et Biophysica Acta. 1265. 173-180 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Cang Chen: "Age-deoendent changes in response of rat prostatic tissues to isoproterenol and forskolin." Mechanisms of Ageing and Development. 81. 1-13 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichiro Amano: "Regulation of phosphorylation of Gi2α protein controls the secretory response to isoproterenol in rat parotid tissues." Biochimica et Biophysica Acta. 1313. 146-156 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Mechanism of β-adrenergic agonist-induced transmural transport of glucose in rat small intestine." Biochimica et Biophysica Acta. 1357. 306-318 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Acetylcholine acts on M_3 muscarinic receptors and induces the translocation of aquaporins5 waater channel via cytosolic Ca^<2+> elevation in rat parotid glands." Biochemica and Biophysical Research Communications. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takafumi Eguchi: "Mechanism underlying histamine-induced desensitization of amylase secretion in rat parotid glands." British Journal of Pharmacology. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 石田 甫(分担): "最新医学大辞典(第2版)" 医歯薬出版株式会社, 2333 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 石田 甫(編集者): "歯科薬理学(第4版)" 医歯薬出版株式会社, 541 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Mechanism of isoproterenol-induced heterologous desensitization of mucin secretion from rat submansibular glands : Regulation of phosphorylation of Gi proteins controls the cell response to the subsequent stimulation." Biochimica et Biophysica Acta. 1265. 173-180 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Cang Chen: "Age-dependent changes in response of rat prostatic tissues to isoproterenol and forskolin : changes with sexual maturation in function of G proteins." Mechanisms of Ageing and Development. 81. 1-13 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichiro Amano: "Regulation of phosphorylation of Gi2alpha protein controls the secretory response to isoproterenol in rat parotid tissues." Biochimica et Biophysica Acta. 1313. 146-156 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Mechanism of beta-adrenergic agonist-induced transmural transport of glucose in rat small intestine : Regulation of phosphorylation of SGLT1 controls the function." Biochimica et Biophysica Acta. 1357. 306-318 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Acetylcholine acts on M_3 muscarinic receptors and induces the translocation of quaporin5 water channel via cytosolic Ca^<2+> elevation in rat parotid glands." Biochemical and Biophysical Research Communications. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takafumi Eguchi: "Mechanism underlying histamine-induced desensitization of amylase secretion in rat parotid glands" British Journal of Pharmacology. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hajime Ishida: Medical Dictionary. Ishiyaku Publishers, Inc.2^<nd>ed., 2333 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hajime Ishida: Dental Pharmacology. Ishiyaku Publishers, Inc.4^<th>ed., 541 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Mechanism of isoproterenol-induced heterologous desensitization of mucin secretion from rat submandibular glands." Biochimica et Biophysica Acta. 1265. 173-180 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Cang Chen: "Age-dependent changes in response of rat prostatic tissues to isoproterenol and forskolin." Mechanisms of Ageing and Development. 81. 1-13 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ichiro Amano: "Regulation of phosphorylation of Gi2α protein controls the secretory response to isoproterenol in rat parotid tissues." Biochimica et Biophysica Acta. 1313. 146-156 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Mechanism of β-adrenergic agonist-induced transmural transport of glucose in rat small intestine." Biochimica et Biophysica Acta. 1357. 306-318 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Acetylcholine acts on M3 muscarinic receptors and induces the translocation of aquaporin5 water channel via cytosolic Ca^<2+> elevation in rat parotid glands." Biochemical and Biophysical Research Communications. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takafumi Eguchi: "Mechanism underlying histamine-induced desensitization of amylase secretion in rat parotid glands." British Journal of Pharmacology. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 石田 甫 (分担): "最新医学大事典(第2版)" 医歯薬出版株式会社, 2333 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 石田 甫 (編著者): "歯科薬理学(第4版)" 医歯薬出版株式会社, 541 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ichiro Amano: "Dephosphorylation of nuclear non-histone proteins in submandibular glands of rats treated with isoproterenol." Research in Experimental Medicine. 194. 185-196 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Mechanisms of isoproterenol-induced heterologous desensitization of mucin secretion from rat submandibular glands." Canadian Journal of Physiology and Pharmacology. 72. 541p (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Mechanism of isoproterenol-induced heterologous desensitization of mucin secretion from rat submandibular glands..." Biochimica et Biophysica Acta. 1265. 173-180 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Cang Chen: "Age-dependent changes in response of rat prostatic tissues to isoproterenol and forskolin:changes with sexual maturation..." Mechanisms of Ageing and Development. 81. 1-13 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ichiro Amano: "Regulation of phosphorylation of Gi2α protein controls the secretory response to isoproterenol in rat parotid tissues." Biochimica et Biophysica Acta. 1313. 146-156 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Mechanism of β-adrenergic agonist-induced transmural transport of glucose in rat small intestine:Regulation of..." Biochimica et Biophysica Acta. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石田甫(編著者): "歯科薬理学(第3版)" 医歯薬出版株式会社, 612 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石田甫(分担): "最新医学大辞典(第2版)" 医歯薬出版株式会社, 2333 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ichiro Amano: "Dephosphorylation of nuclear non-histone proteins in submandibular glands of rats treated with isoproterenol." Reserach in Experimental Medicine. 194. 185-196 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Mechanisms of isoproterenol-induced heterologous desensitization of mucin secretion from rat submandibular glands." Canadian Journal of Physiology and Pharmacology. 72. 541- (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Amano: "Phosphorylation of Gi proteins is important in the mechanism of supersensitivity of amylase secretion from rat parotid tissues treaded briefly with isoproterenol." Canadian Journal of Physiology and Pharmacology. 72. 558 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Mechanism of isoproterenol-induced heterologous desensitization of mucion secretion from rat submandibular glands..." Biochimica et Biophysica Acta. 1265. 173-180 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Cang Chen: "Age-dependent changes in response of rat prostatic tissues to isoproterenol and forskolin : changes with sexual maturation..." Mechanisms of Ageing and Development. 81. 1-13 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Amano: "Regulation of phosphorylation of Gi2α protein controls the secretory response to isoproterenol in rat parotid tissues." Biochimica et Biophysica Acta. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 石田 甫: "歯科薬理学(第4版)" 医歯薬出版株式会社(印刷中), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Amano: "DephosPhorylation of nuclear non-histone proteins in submandibular glands of rats treated with isoproternol." Research in Experimental Medicine. 194. 185-196 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Mechanism of isoproterenol-induced heterologous desensitization of mucin secretion from rat submandibular glands." Canadian Journal of Physiology and Pharmacology. 72. 541 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ichiro Amano: "Phosphorylation of Gi proteins is important in the mechanism of supersensitiyity of amylase secretion from rat parotid tissues treated briefly with isoproterenol." Canadian Journal of Physiology and Pharmacology. 72. 558 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] 天野,伊知郎: "β-作動薬によるムチン分泌のDesensitizationとその機序" 日本唾液腺学会誌. 35. 69-71 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Mechanism of isoproterenol-induced beterologous desensitization of mucin secretion from rat submandibular gloands:Regulation of phosphorylation ofGi proteins controls the cell response...." Biochimica et Biophysica Acta. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 天野,伊知郎: "β-作動薬によるアミラーゼ分泌の感作・脱感作現象の分子機構" 日本唾液腺学会誌. 36(in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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