| Project/Area Number |
06404070
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Aichigakuin University |
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Principal Investigator |
HASEGAWA Jiro Dent.Mate.Scie., Aichigakuin Univ.Professor, 歯学部・歯科理工学, 教授 (10064809)
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| Co-Investigator(Kenkyū-buntansha) |
BAN Seiji Dent.Mate.Scie., Aichigakuin Univ.Assistant Professor, 歯学部・歯科理工学, 講師 (10159105)
KAWAI Tatshushi Dent.Mate.Scie., Aichigakuin Univ.Assistant Professor, 歯学部・歯科理工学, 講師 (60167351)
TAKAHASHI Yoshifumi Dent.Mate.Scie., Aichigakuin Univ.Associate Professor, 歯学部・歯科理工学, 助教授 (00090142)
FUKUI Hisao Dent.Mate.Scie., Aichigakuin Univ.Associate Professor, 歯学部・歯科理工学, 助教授 (50090147)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥27,200,000 (Direct Cost: ¥27,200,000)
Fiscal Year 1996: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1995: ¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1994: ¥11,400,000 (Direct Cost: ¥11,400,000)
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| Keywords | Implant / Biomaterial / BMP / bone formation / functional material / bone reconstruction / transplant / osteoinduction |
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| Research Abstract |
In order to make composite materials of BMP-biomaterial which possess the ability to induce new bone in living human tissues, wide range of biomaterials were screening, then combined with BMP.It was necessary to make large amount of BMP to perform these experiments which varies many polymers, metals and ceramics. Factory scale of extraction system for half purified BMP was established, then water soluble BMP was produced stable. Furthermore, making of recombinant BMP was also tried in Baculo Virus System. The Baculo Virus was infected to the sf-9 cell (insect cell line for cell culture) then recombinant BMP was collected from both culture medium and from cell body. The activity of recombinant-BMP was 1/10 of half purified BMP derived natural bone. The physical property of recombinant BMP and water soluble BMP was almost same, therefore, water soluble BMP was used for the combination with the biomaterials.
Gelatin, chitin, agarose, starch and atelocollagen were experimented for the carrier of BMP,those showed excellent enhancement of BMP activity. The affinity of BMP to ceramics was investigated for the combination of BMP to ceramics which were used as framework of new bone formation. Calcium phosphate, titania, zirconia showed specific affinity which determine by the polyclonal antibody. The combination of BMP with metals were also carried out. Titanium showed excellent combination with BMP and the surface treatment modified remarkably the osteoinductive activity of BMP-metal composites.
With these results which were obtained in this project will be important information for the clinical usage in very near future.
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