| Project/Area Number |
06404084
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Tohoku University |
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Principal Investigator |
IDE Hiroyuki Faculty of Science, Tohoku University , Professor, 理学研究科, 教授 (70022704)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Naoyuki School of Human Science, Waseda University, Research Associate, 人間科学部, 助手 (50267449)
TAMURA Koji Faculty of Science, Tohoku University, Research Associate, 理学研究科, 助手 (70261550)
YAMAMOTO Hiroaki Faculty of Science, Tohoku University , Associate Professor, 理学研究科, 助教授 (40174809)
渡辺 明彦 山形大学, 理学部, 助手 (30250913)
大椙 弘順 東北大学, 理学部, 助手 (00201377)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥21,300,000 (Direct Cost: ¥21,300,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥17,700,000 (Direct Cost: ¥17,700,000)
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| Keywords | cell adhesion molecule / cell sorting / cadherin / limb bud / pattern formation / 位置価 |
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| Research Abstract |
In vertebrate limb development, limb mesenchyme cells in the progress zone form cartilage pattern depending on the positional values allotted to them. The limb bud cells are though to use cell surface molecules to recognize the positional values of their surrounding cells.
We have found the difference in cell surface molecules from the cell sorting experiment using chick limb mesenchyme with different positional values. The sorting out in monolayr culture occurred when the mesenchymal cells at different developmental stages or from different positions were mixed. The sorting out occurred independently of chondrogenic potency and cartilage nodules were formed in the center of each sorted area. The mesenchymal cells with different positional values were sorted even on agar where cells could not attach and form aggregates, suggesting the sorting out occurs depending on the difference on not cell-substratum but cell-cell affinity.
To identify cell adhesion molecules which control the positional value-related cell adhesion, we first cloned cDNA of cadherin superfamily genes expressing in chick limb bud with RT-PCR.Protocadherin 2, a homologue of human protocadherin Pcdh2, was identified. The expression pattern of protocadherin 2 suggests its close relationship to cell sorting.
We have raisd a monoclonal antibody to the cell surface molecular of chick limb bud mesenchyme, which inhibited the sorting out between the progress zone cells at different developmental stages.
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