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Evolution of visual pigment and differentiation of membrane structure : Production of monoclonal antibody and electron microscopic immnunohistochemistry

Research Project

Project/Area Number 06405001
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionYamagata University

Principal Investigator

TONASAKI Akira  Yamagata University school of medicine, Professor, 医学部, 教授 (90004572)

Co-Investigator(Kenkyū-buntansha) MORI Keiji  Yamagata University school of medicine, Assistant, 医学部, 助手 (40250914)
SASASKI Katsunori  Yamagata University school of medicine, Associate Professor, 医学部, 助教授 (30170666)
TOKENAGA Fumio  Osaka University, Faculty of Science, Professor, 理学部, 教授 (80025452)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥29,400,000 (Direct Cost: ¥29,400,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥25,000,000 (Direct Cost: ¥25,000,000)
Keywordsretina / lamprey / visual pigment / monoclonal antibody / rhodopsin / arrestin / immmunohistochemisity / 電顕免疫組織化学 / 光受容細胞の分化 / 光受容細胞 / モノクローン抗体 / 免疫電顕組織化学
Research Abstract

1.Production of monoclonal antibodies against lamprey-retinal homogenate.
We have developed 10 new monoclonal antibodies. Their binding sites were detected on the sectioned retina by optical and electron microscopic immunohistochemistry. Molecular weight of antigens were estimate by western blotting.
1) W26, W27, W39 : Specifically bound to the outer segment of short photoreceptor cell (SPC).
2) W226, W231, W312, W425 : To the outer segment, inner segment except for ellipsoid of SPC and axon.
3) W15 : To the outer and inner segment of SPC.
4) W38 : To the all region of SPC.
5) W318 : To the neural layr of retina.
Following 3 monoclonal antibodies were specialized for a molecular component involved in photoreceiving procedure.
1) W39 : For lamprey rhodopsin, molecular weight is about 40kDa.
2) W312, W425 : For lamprey arrestin, molecular weight is about 49kDa.
2.Establishment of quantitative immunogold electron microscopy.
The specimen was fixed with 4% paraformaldehyde and embedded in LR-white. There were considerably remakable washing out and trace of destruction in the neural layr of retina, and deformation of outer segment of photoreceptor cells as compared with conventional fixation. As far as photoreceptor cells are concerned, however, fortuitously preserved outer segments were found as assosiated variously with fine gold particles, allowing our quantitative examinatoin.
Conculusion
A series of anti-lamprey retinal antibodries were produced and used for recognition of protein, glycoconjugate and lipids in retinal cells. These antibodies are apparently useful as probes for relationship between corresponding cells of different species.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Katori Y.,Tonosaki A.,Takasaka T: "WGA lectin binding sites of the apical surface of,Corti epithelium : Enhancement by back-scattered electron imaging in guinea-pig inner ear." J.Electron Microsc.45. 207-212 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa M.,Johnson LV.,DeWing MD.,Koseki T.,Tonosaki A.,Sakuragi S: "pH-dependent changes in interphotoreceptor matrix domains surrounding cone photoreceptors." Ophthalmic Res.28. 117-124 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito M.,Inanobe A.,Horio Y.,Isomoto.,Ito H.,Mori K.,Tonosaki A.,Tomoike H.,Kurachi Y: "Immunolocalization of an in wardly rectifying K^+ channel,Kab-2 (Kir4.1),in the basolateral memburane of renal distal tubular epithelia." FEBS Letters. 388. 11-15 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa M.,Tonosaki A.,Hisatomi O.,Tokunaga F.,Kokeki T.,Sakuragi S: "Lectin cytochemical analysis of glycoconjyugate in photoreceptor cell memburanes of Lampetra japonica." Vision Res.36. 1513-1520 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamaguti K.,Takahashi S.,Takahashi Y.,Shoji T.,Yuki Y.,Sasaki K.,Tonosaki A: "Disteibution of intercellular adhesin molecule-1 on leukocytes and corneal ednodthelium after endtoxin stimulation in rats." Internat Ophthalmol. 119. 303-306 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki K.,Okouchi Y.,Rothkotter H-J.,Pabst R: "Ultrastructural localization of intercllular adhesion molecule (ICAM-1) on the cell surface of high endothelial venules in lymph nodes." Anat.Rec.244. 105-111 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamaguchi K,Takahashi S,Sasaki K,Tonosaki A: "Immuno-scanning electron microscopical analysis of intercellular adhesion molecule-1 in teh corneal endotheiium involved in endotoxin induced uveitis. (Abs)" Invest.Ophthalmol.Vis.Sci.36. 294- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hattori R,Watanabe H,Tonosaki A,Hattori T: "Unusual morphology of Agromonas oiigotrophica and the effect of NaCl and organic nutrient on its fine structure." J.Gen.Appl.Microbiol.41. 23-30 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shao F,Tonosaki A,Watanabe Y: "Vincula tendinum of human hands with special reference to vascular patency." Acta Anat.Nippon.70. 569-576 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Katori Y,Tonosaki A,Takasaka T: "WGA lectin binding sites of the apical surface of Corti epithelium : Enhancement by back-scattered electron imaging in guinea-pig inner ear." J.Electr.Microsc.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida M,Tonosaki A: "Anti-lamprey (Lampetra japonica) retinal antibodies which distinguish between short and long photoreceptor cells." Comp.Biochem.Physiol.vol.107B. 183-188 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Katori Y,Takasaka T,Ishikawa M,Tonosaki A: "Fine structure and lectin histochemistry of the apical surface of the free neuromast of Lampetra japonica." Cell Tiss.Res.vol.276. 245-252 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe H,Tonosaki A: "Gap junction in the apotosis : TEM observation of membrana-granulosa cells of mouse ovarian follicle." In : Progress in Cell Research vol.4,Y.Kanno (ed.),Elsevier Science B.V.,. vol.4. 37-40 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe H,Koike K,Tonosaki A: "Formation and degradation of gap junctions : Review." Yamagata Med.J.vol.12. 87-98 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 外崎 昭(分担執筆): "岩波生物学辞典,第4版" 岩波書店, (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Katori Y., Tonosaki A., Takasaka T: "WGA lectin binding sites of the apical surface of Corti epithelium : Enhancement by back-scattered electron imaging in guinea-pig inner ear." J.Electron Microsc.45. 207-212 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa M., Johnson LV., DeWing MD., Koseki T., Tonosaki A., Sakuragi S: "pH-dependent changes in interphotoreceptor matrix domains surrounding cone photoreceptors." Ophthalmic Res.28. 117-124 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito M., Inanobe A., Horio Y., Isomoto., Ito H., Mori K., Tonosaki A., Tomoike H., Kurachi Y: "Immunolocalization of an inwardly rectifying K^+ channel, Kab-2 (Kir4.1), in the basolateral memburane of renal distal tubular epithelia." FEBS Letters. 388. 11-15 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa M., Tonosaki A., Hisatomi O., Tokunaga F., Kokeki T., Sakuragi S: "Lectin cytochemical analysis of glycoconjyugates in photoreceptor cell memburanes of Lampetra japonica." Vision Res.36. 1513-1520 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamaguti K., Takahashi S., Takahashi Y., Shoji T., Yuki Y., Sasaki K., Tonosaki A: "Disteibution of intercellular adhesin molecule-1 on leukocytes and corneal ednodthelium after endtoxin stimulation in rats." Internat Ophthalmol. 119. 303-306 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sasaki K., Okouchi Y., Rothkotter H-J., Pabst R: "Ultrastructural localization of intercllular adhesion molecule (ICAM-1) on the cell surface of high endothelial venules in lymph nodes." Anat.Rec.244. 105-111 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamaguchi K,Takahashi S,Sasaki K,Tonosaki A: "Immuno-scanning electron microscopical analysis of intercellular adhesion molecule-1 in the corneal endothelium involved in endotoxin induced uveitis." Invest Ophthalmol Vis Sci. 36. 294 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hattori R,Watanabe H,Tonosaki A,Hattori T: "Unsual morphology of Agromonas oligotrophica and the effect of NaCl and organic nutrient on its fine structure." J.Gen.Appl.Microbiol.41. 23-30 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shao F,Tonosaki A,Watanabe Y: "Vincula tendinum of human hands with special reference to vascular patency." Acta.Anat.Nippon. 70. 569-576 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe H,.Tonosaki A: "Gap junction in the apotosis : TEM observation of membrana-granulosa cells of mouse ovarian follicle." In : Progress in cell research vol.4 Y.Kanno (ed.), Elsevier Science B,V.37-40 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida M., Tonosaki A: "Anti-lamprey (Lampetra Japonica) retinal antibodies which distinguish between shrot and long photoreceptor cell." Comp Biochem Physiol. 107B. 183-188 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Katori Y., Takasaka T., Ishikawa M., Tonosaki A: "Fine structure and lectin histochemistry of the apical surface of the free neuromast of Lampetra japonica." Ceii Tissue Res.276. 245-252 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe H,.Koike K., Tonosaki A: "Formation and degradation of gap junctions." Yamagata Med.j.12. 87-98 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Katori Y.,Tonosaki A.,Takasaka T: "WGA lectin binding sites of the apical surface of Corti epithelium : Enhancement by back scattered electron imaging in guinea-pig inner ear." J.Electron Microsc. 45. 207-212 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa M.,Johnson LV.,DeWing MD.,Koseki T.,Tonosaki A.,Sakuragi S: "pH-dependent changes in interphotoreceptor matrix domains surrounding cone photoreceptors." Ophthalmic Res. 28. 117-124 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito M.,Inanobe A.,Horio Y.,Isomoto Ito H.,Mori K.,Tonosaki A.,Tomoike H.,Kurachi Y: "Immunolocalization of an in wardly rectifying K^+ channel,Kab-2(Kir4.1),in the basolateral memburane of renal distal tubular epithelia." FEBS Letters. 388. 11-15 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa M.,Tonosaki A.,Hisatomi O.,Tokunaga F.,Kokeki T.,Sakuragi S: "Lectin cytochemical analysis of glycoconjyugates in photoreceptor cell memburanes of Lampetra japonica." Vision Res. 36. 1513-1520 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguti K.,Takahashi S.,Takahashi Y.,Shoji T.,Yuki Y.,Sasaki K.,Tonosaki A: "Disteibution of intercellular adhesin molecule-1 on leukocytes and corneal ednodthelium after endtoxin stimulation in rats." Internat Ophthalmol. 119. 303-306 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki K.,Okouchi Y.,Rothkotter HJ.,Pabst R: "Ultrastructural localization of intercllular adhesion molecule(ICAM-1)on the cell surface of high endothelial venules in lymph nodes." Anat.Rec. 244. 105-111 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 外崎昭(分担執筆): "岩波生物学辞典,第4版" 岩波書店, (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi K, Takahashi S, Sasaki K, Tonosaki A: "Immuno-scanning electron microscopical analysis of intercellular adhesion molecule-1 in the corneal endothelium involved in endotoxin induced uveitis.(Abs)" Invest. Ophthalmol. Vis. Sci.36. 294 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hattori R, Watanabe H, Tonosaki A, Hattori T: "Unusual morphology of Agromonas oligotrophica and the effect of NaCl and organic nutrient on its fine structure." J. Gen. Appl. Microbiol.41. 23-30 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shao F, Tonosaki A, Watanabe Y: "Vincula tendinum of human hands with special reference to vascular patency." Acta Anat. Nippon.70. 569-576 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Katori Y, Tonosaki A, Takasaka T: "WGA lectin binding sites of the apical surface of Corti epithelium: Enhancement by back-scattered electron imaging in guinea-pig inner ear." J. Electr. Microsc.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishikawa M, Johnson LV, DeWing MD, Koseki T, Tonosaki A, Sakuragi S: "pH-dependent changes in interphotoreceptor matrix domains surrounding cone photoreceptors." Ophthalmic Res.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamaguchi K, Takahashi S, Takahashi Y, Shoji T, Yuki Y, Sasaki K, Tonosaki T: "Distribution of intercellular adhesion molecule-1 on leukocytes and corneal ednodthelium after endotoxin stimulation in rats." Internat. Ophthalmol.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamaguchi K, Takahashi S, Sasaki K, Tonosaki A: "Early expression of intercellular adhesion molecule-1 in the corneal edndothelium stimulated by edotoxin: An immuno-scanninng electron microscopical analysis." Jap. J. Ophthalmol.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshida M,Tonosaki A: "Anti-lamprey (Lampetra japonica)retinal antibodies which distinguish between short and long photoreceptor cells." Comp.Biochem.Physiol.107B. 183-188 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katori Y,Takasaka T,Ishikawa M,Tonosaki A: "Fine structure and lectin histochemistry of the apical surface of thefree neuromast of Lampetra japonica." Cell Tiss.Res. 276. 245-252 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Watanabe H,Tonosaki A: "Gap junction in the apotosis:TEM observation of membrana-granulosa cells of mouse ovarian follicle." In:Progress in Cell Research vol.4,Y.Kanno(ed.),EIsevier Science B.V.,. 4. 37-40 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Watanabe H,Koike K,Tonosaki A: "Formation and degradation of gap junctions:Review." Yamagata Med.J.12. 87-98 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamaguchi K,Takahashi S,Sasaki K,Tunosaki A: "Immuno-scanning electron microscopical analysis of in tercellular adhesion molecule-1 in the corneal endothelium involved.in endotoxin induced uveitis." ARVO 1995. (発表予定).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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