Project/Area Number |
06452442
|
Research Category |
Grant-in-Aid for General Scientific Research (B)
|
Allocation Type | Single-year Grants |
Research Field |
Biophysics
|
Research Institution | UNIVERSITY OF TOKYO |
Principal Investigator |
SUYAMA Akira COLLEGE OF ARTS AND SCIENCES INSTITUTE OF PHYSICS,UNIVERSITY OF TOKYO ASSOCIATE PROFESSOR, 教養学部, 助教授 (90163063)
|
Project Period (FY) |
1994 – 1995
|
Project Status |
Completed (Fiscal Year 1995)
|
Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥5,800,000 (Direct Cost: ¥5,800,000)
|
Keywords | DNA PROBE / BIOCHIP / BASE SEQUENCE / HYBRIDIZATION / ハイブリダイゼーション |
Research Abstract |
DNA probe integrated chips are silicon-or glass-based small surfaces on which many DNA oligonucleotides are immobilized. The chips provide a basic technology to perform clone-ordering for genome mapping, high-throughput sequencing by SBH, DNA diagnosis and gene expression profile analysis. In the present study, we have developed the light-directed oligonucleotide immobilization method for making DNA probe integrated chips. The light-directed oligonucleotide immobilization method integrates presynthesized DNA oligonucleotides and PCR products at the addressed positions on solid support. So it can make DNA probe integrated chips with DNA probes long enough to identify a particular gene of a large genome precisely.
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