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DNAプローブ集積チップによる塩基配列の高速探査

Research Project

Project/Area Number 06452442
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

SUYAMA Akira  COLLEGE OF ARTS AND SCIENCES INSTITUTE OF PHYSICS,UNIVERSITY OF TOKYO ASSOCIATE PROFESSOR, 教養学部, 助教授 (90163063)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsDNA PROBE / BIOCHIP / BASE SEQUENCE / HYBRIDIZATION / ハイブリダイゼーション
Research Abstract

DNA probe integrated chips are silicon-or glass-based small surfaces on which many DNA oligonucleotides are immobilized. The chips provide a basic technology to perform clone-ordering for genome mapping, high-throughput sequencing by SBH, DNA diagnosis and gene expression profile analysis. In the present study, we have developed the light-directed oligonucleotide immobilization method for making DNA probe integrated chips. The light-directed oligonucleotide immobilization method integrates presynthesized DNA oligonucleotides and PCR products at the addressed positions on solid support. So it can make DNA probe integrated chips with DNA probes long enough to identify a particular gene of a large genome precisely.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Morimoto,N.and Suyama,A.: "Rapid oligonucleotide hybridization on microfiltration plates." Nucleic Acids Symposium Series. No.31. 119-120 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suyama,A.: "DNA sequencing by oligonucleotide hybridization." Gendai Kagaku. No.274. 8-9 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suyama,A.: "DNA Chips-Integrated Chemical Circuits for DNA Diagnosis and DNA Computers." (to be submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohno,H., Sugiyama,T., Suyama,A.: "Photolithographic integration of presynthesized DNA oligonucleotides on a glass plate." (to be submitted.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Morimoto, N.and Suyama, A.: "Rapid oligonucleotide hybridization on microfiltration plates." Nucleic Acids Symposium Series. No.31. 119-120 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suyama, A.: "DNA sequencing by oligonucleotide hybridization." Gendai Kagaku. No.274. 8-9 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suyama, A.: "DNA Chips-Integrated Chemical Circuits for DNA Diagnosis and DNA Computers." (to be submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohno, H., Sugiyama, T.and Suyama, A.: "Photolithographic Integration of presynthesized DNA oligonucleotides on a glass plate using photolithography." (to be submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Morimoto,N.& Suyama,A.: "Rapid oligonucleotide hybridization on microfiltration pla" Nucleic Acids Symposium Series. 31. 119-120 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 陶山明: "オリゴヌクレオチドとのハイブリッド形成によるDNA塩基配列決定" 現代化学. 274. 8-9 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suyama,A.& Matsuura,M.: "Light-directed terminal immobilization of DNA probes on sol surfaces." DNA Research. (投稿予定).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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