• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

X-RAY STRUCTURAL ANALYSES OF THIN FILAMENTS IN A SKELETAL MUSCLE DURING CONTRACTION AND MYOSIN HEADS DURING AN HYDROLYSIS OF ATP

Research Project

Project/Area Number 06452443
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

WAKABAYASHI Katsuzo  FACULTY OF ENGINEERING SCIENCE,BIOPHYSICAL ENGINEERING,ASSOCIATE PROFESSOR, 基礎工学部, 助教授 (00029521)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsX-Ray Diffraction / Structural Analyzes / Muscle Contraciton / Thin Filament Structure / Myosin Head / Actomyosin Complex / Synchrotron Radiation / Imaging Plate / X線回折
Research Abstract

[1] Structural change of the thin filaments during contraction of frog skeletal muscle
Firstly, we measured precisely the spacing changes of the actin filament-based reflections in the X-ray diffraction pattern from an isometrically contracting muscle using synchrotron radiation. We found 0.2-0.3% of extnsion in the actin filaments as well as the myosin filaments. Such filament extensibility was elastic. These results provided a great insight to a force-generation mechanism in muscle. Secondly, we measured intensities of the thin filament-based layr lines up to -13A.Using the atomic data of the actin monomer and tropomyosin, we constructed a model of the thin filament and calculated a difference Fourier, indicating that the structural change occurred within the thin filaments. Modeling studies revealed distinct changes of the domain structure of actin in the filament which were induced by interaction with myosin heads. Tropomyosin molecules also moved in the azimuthal direction by about … More 5A,less than previously postulated in a steric block hypothesis of muscle regulation.
[2] Conformational changes of the myosin head and the solution structure of an actomyosin complex
Synchrotron X-ray solution scattering revealed that the myosin head altered its conformation during an hydrolysis of ATP : the radius of gyration and maximum chord length decreased by 3A and 10A,respectively. Modeling studies using the atomic data indicated that the light chain-binding domain rotated around the amino acid 711 by 12゚ downward by 10゚ in the plane including its long axis. From the experiments with nucleotide analogs, the conformational changes occurred in the ADP.P_i state and reversed in the product release steps. We succeeded in preparing non-polymerized actin-myosin head complex and investigated the solution structure of this complex by X-ray solution scattering. The result indicated that the actin monomer bound to the proximal end of the catalytic domain of the myosin head with a center-to-center distance of 75A.Such studies have opened a very important root to structural studies of the smallest energy transducing unit in the actomyosin system. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] Y. Ueno: "Modelling Structural Changes of the Muscle Thin Filaments during an Isometric Contraction" Synchrotron Radiation in the Biosciences eds. (B. Chance et al.), Oxford Univ. Press, Oxford. 1. 443-450 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Mitsui: "Frequency Dependence of the Variation of the X-ray Diffracion Pattern from Tetanized Frog Skeletal Muscle during Sinusoidal Length Chage" Synchrotron Radiation in the Biosciences eds. (B. Chance et al.), Oxford Univ. Press, Oxford. 1. 460-472 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Horiuti: "Structutal Changes of Crossbridge on Contaction and Relaxation Induced by Photolysis of Caged ATP" Synchrotron Radiation in the Biosciences eds. (B. Chance et al.), Oxford Univ. Press, Oxford. 1. 486-492 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Tokunaga: "Structural Change of the Myosin Head Detected by Electron Microscopy and Small-Angle X-ray Scattering" Synchrotron Radiation in the Biosciences eds. (B. Chance et al.), Oxford Univ. Press, Oxford. 1. 493-501 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Tajima: "X-ray diffraction Studies of the Thin Filaments in a Contracting Molluscan Smooth Muscle" Synchrotron Radiation in the Biosciences eds. (B. Chance et al.), Oxford Univ. Press, Oxford. 1. 509-518 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Horiuti: "X-ray Equatorial Diffraction during ATP-Induced Ca^<2+>-Free Muscle Contraction and the Effect of ADP" J. Biochem.115. 953-957 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Wakabayashi: "X-ray Diffraction Evidence for the Extensibility of Actin and Myosin Filaments during Muscle Contraction" Biophys. J.67. 2422-2435 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Tajima: "X-ray Evidence for the Elongation of Thin and Thick Filaments during Isometric Contraction of a Molluscan Smooth Muscle" J. Muscle Res. Cell Motility. 15. 659-671 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 若林克三: "X線溶液散乱法で捉えたエネルギー変換中のミオシン頭部の構造変化" 生物物理. 34. 131-135 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 若林克三: "シンクロトロンX線回析による筋収縮の研究" SR科学技術情報. 4. 7-16 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Amemiya: "Large-aperture TV detector with a beryllium-windowed image intensifier for X-ray diffraction" Rev. Sci. Instrum.68(2). 2290-2294 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Sugimoto: "Conformational changes of the myosin heads during hydrolysis of ATP as analyzed by X-ray solution" Biophys. J.58. 29s-34s (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H. Iwamoto: "Effect of Stretch and Release on Equatorial X-ray Diffraction during a Twitch Contraction of Frog Skeletal Muscle" Biophys. J.68. 227-234 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N. Yagi: "A real-time observation of X-ray diffraction from frog skeletal muscle during and after slow length changes" Japan. J. Physiol.45. 583-606 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 雨宮慶幸: "X線回析実験用のテレビ型2次元X線検出器の開発" Photon Factory News. 13. 21-24 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 若林克三: "時分割小角X線散乱による低次構造の動的変化" KEK Proceedings. 95-8. 32-35 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H. Tanaka: "The time for movement of myosin heads from thick to thin filaments is negligibly small" Proc. ICSRS-AFSR'95. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Horiuti et al.: "X-ray equatorial diffraction during ATP-induced Ca^<2+>-free contraction and the effect of ADP" J.Biochem.115. 953-957 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Wakabayashi et al.: "X-ray diffraction evidence for the extensibility of actin and myosin filaments during muscle contraction" Biophys.J.67. 2422-2435 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Tajima et al.: "X-ray evidence for the elongation of thin and thick filaments during isometric contraction of a molluscan smooth muscle" J.Muscle Res.Cell Motility. 15. 659-671 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Iwamoto et al.: "Effects of stretch and release on equatorial X-ray diffraction during a twitch contraction of frog skeletal muscle" Biophys.J.68. 227-234 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Amemiya et al.: "Large-apeture TV detector with a beryllium-windowed image intensifier for X-ray diffraction" Rev.Sci.Instrum.68(2). 2290-2294 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Sugimoto et al.: "Conformational changes of the myosin heads during hydrolysis of ATP as analyzed by X-ray solution scattering" Biophys.J.68. 29s-34s (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Yagi et al.: "A real-time observation of X-ray diffraction from frog skeletal muscle during and after slow length changes" Japan.J.Physiol.45. 583-606 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Yagi et al.: "Small-angle X-ray diffraction of muscle using undulator radiation from the Tristan main ring at KEK" J.Synchrotron Radiation. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 若林克三: "X線溶液散乱法で捉えたエネルギー変換中のミオシン頭部の構造変化" 生物物理. 34. 131-135 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 若林克三: "シンクロトロンX線回折による筋収縮の研究" SR科学技術情報. 4. 7-16 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Amemiya: "Large-aperture TV detector with a beryllium-windowed image intensifier for X-ray diffraction" Rev.Sci.Instrum.68(2). 2290-2294 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Sugimoto: "Conformational changes of the myosin heads during hydrolysis of ATP as analyzed by X-ray solution" Biophys.J.58. 29s-34s (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N.Yagi: "A real-time observation of X-ray diffraction from frog skeletal muscle during and after slow length changes" Japan.J.Physiol.45. 583-606 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 雨宮慶幸: "X線回折実験用のテレビ型2次元X線検出器の開発" Photon Factory News. 13. 21-24 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 若林克三: "時分割小角X線散乱による低次構造の動的変化" KEK Proceedings. 95-8. 32-35 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Tanaka: "The time for movement of myosin heads from thick to thin filaments is negligibly small" Proc.ICSRS-AFSR'95. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Ueno: "Modelling Structural Changes of the Muscle Thin Filaments during an Isometric Contraction" Synchrotron Radiation in the Biosciences eds.(B.Chance et al.),Oxford Univ.Press,Oxford. 1. 443-450 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Mitsui: "Frequency Dependence of the Variation of the X-ray Diffracion Pattern from Tetanized Frog Skeletal Muscle during Sinusoidal Length Change" Synchrotron Radiation in the Biosciences eds.(B.Chance et al.),Oxford Univ.Press,Oxford. 1. 460-472 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Horiuti: "Structutal Changes of Crossbridge on Contaction and Relaxation Induced by Photolysis of Caged ATP" Synchrotron Radiation in the Biosciences eds.(B.Chance et al.),Oxford Univ.Press,Oxford. 1. 486-492 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Tokunaga: "Structutal Change of the Myosin Head Detected by Electron Microscopy and Small-Angle X-ray Scattering" Synchrotron Radiation in the Biosciences eds.(B.Chance et al.),Oxford Univ.Press,Oxford. 1. 493-501 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Tajima: "X-ray Diffraction Studies of the Thin Filaments in a Contracting Molluscan Smooth Muscle" Synchrotron Radiation in the Biosciences eds.(B.Chance et al.),Oxford Univ.Press,Oxford. 1. 509-518 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Horiuti: "X-ray Equatorial Diffraction during ATP-Induced Ca^<2+>-Free Muscle Contraction and the Effect of ADP" J.Biochem.115. 953-957 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Wakabayashi: "X-ray Diffraction Evidence for the Extensibility of Actin and Myosin Filaments during Muscle Contraction" Biophy J.67. 2422-2435 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Tajima: "X-ray Evidence for the Elongation of Thin and Thick Filaments during Isometric Contraction of a Molluscan Smooth Muscle" J.Muscle Res.Cell Motility. 15. 659-671 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Iwamoto: "Effect of Stretch and Release on Equatorial X-ray Diffraction during a Twitch Contraction of Frog Skeletal Muscle" Biophys J.68. 227-234 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Amemiya: "Large-Aperture TV Detector with a Beryllium-Windowed Image Intensifier for X-ray Diffraction" Rev.Sci.Instrum.(in prees). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Sugimoto: "Conformational Change of the Myosin Heads during Hydrolysis of ATP by X-ray Solution Scattering" Biophys.J.(in prees). (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi