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Development of New Sensing Methods for Bioactive Ions and Molecules by Mimicking Homeostasis in Biological Membranes

Research Project

Project/Area Number 06453067
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 分離・精製・検出法
Research InstitutionThe University of Tokyo

Principal Investigator

SUGAWARA Masao  The University of Tokyo, Graduate School of Science, 大学院・理学系研究科, 助教授 (50002176)

Co-Investigator(Kenkyū-buntansha) TOHDA Kouji  The University of Tokyo, Graduate School of Science, 大学院・理学系研究科, 助手 (60212065)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsCalmodulin / synthetic peptide / surface plasmon resonance / active transport / dopamine / Lasalocid A / planar bilayer / synthetic receptor / ホメオスタシス / M13 / カルシウムセンサ / バイオセンサー
Research Abstract

New Sensing Membranes based on the principle of homeostasis have been developed.
1) An optical sensor has been described by using calmodulin (isolated and purified from calf brain) and a synthetic peptide M13. Formation of a ternary complex between Ca^<2+>/calmodulin and M13 was monitored by using the surface plasomon resonance method. A highly sensitive and selective detection of several tens nM Ca^<2+> ions was demonstrated.
2) An active transport system for dopamine was constructed by using lasalocid as a carrier dissolved in liquid membranes. Enhancement of concetratio of dopamine at nM levels was achived by using the active transport system.
3) Direct incorporation of synthetic receptors into planar bilayer lipid membranes formed by the folding method was successfully achieved. New potentiometric sensing systems were designed by using these receptor-incorporated BLMs.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] M.Sugawara: "Concentration of Dopamine by Proton-Driven Uphill Transport Using Lasalocid A as the Carrier" Anal.Sci.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Yagi: "Channel Mimetic Sensing Membranes for Alkali Metal Cations Based on Oriented Monolayers of Calixarene Esters." J.Electroanal.Chem.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Namba: "Molecular Resolution Images of a Calix 〔6〕 arene from Atomic Force Microscopy" Langmuir. 11. 635-638 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Sugawara: "Frontiers in Biosensors" F.D.Scheller (ed.)(in press), (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Sugawara, K.Okude, K.Tohda, Y.Umezawa: "Concentration of Dopamine by Proton-Driven Uphill Transport Using Lasalocid A as the Carrier" Anal.Sci.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Yagi, S.B.Khoo, M.Sugawara, T.Sakaki, S.Shinkai, K.Odashima, Y.Umezawa: "Channel Mimetic Sensing Membranes for Alkali Metal Cations Based on Oriented Monolayrs of Calixarene Esters" J.Electroanal.Chem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Namba, M.Sugawara, P.Buhlmann, Y.Umezawa: "Molecular Resolution Images of a Calix [6] arene from Atomic Force Microscopy" Langmuir. 11. 635-638 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Sugawara, H.Sato, T.Ozawa, Y.Umezawa: Receptor Based Chemical Sensing, in Frontiers in Biosensors. F.D.Scheller (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Sugawara: "Concentration of Dopamine by Proton-Driven Uphill Transport Using Lasalocid A as the Carrier" Anal.Sci.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Yagi: "Channel Mimetic Sensing Membranes for Alkali Metal Cations Based on Oriented Monolayers of Calixarene Esters." J.Electroanal.chem.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Namba: "Molecular Resolution Images of a Calix[6]arene from Atomic Force Microscopy" Langmuir. 11. 635-638 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Sugawara: "Frontiers in Biosensors" F.D.Sheller(ed.)(in press), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Sugawara: "Factors Affecting Background Permeabilities of Ordered Mono-Bi-and Multilayers as Channel Mimetic Sensing Membranes" Anal.Sci.10. 343-347 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Odashima: "Channel Mimetic Sensing Membranes Based on Host-Guest Molecular Recognition by Synthetic Receptors" Interfacial Design and Chemical Sensing. 123-134 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 遠田浩司: "膜を横切る信号の伝達に基づく化学センシングの新しい展開" 膜. 19. 122-132 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Namba: "Molecular resolution images of a calix[6]arene from atomic force microscopy" Langmuir. 11(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Sugawara: "Current Topics in Biophysics" 未定, (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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