| Project/Area Number |
06453072
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分離・精製・検出法
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| Research Institution | School of Pharmacy, Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
TAKAMURA Kiyoko School of Pharmacy, Tokyo University of Pharmacy and Life Science, Professor, 薬学部, 教授 (40057302)
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| Co-Investigator(Kenkyū-buntansha) |
KUSU Fumiyo School of Pharmacy, Tokyo University of Pharmacy and Life Science, Associate Pro, 薬学部, 助教授 (70057371)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1994: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Voltammetry / Flow injection analysis / High-performance liquid chromatography / Electrochemical detection / Acid value of fat and oil / Higher fatty acid / Lipase activity / Cholinesterase activity / 油脂中の遊離脂肪酸の定量 / フローインジェクションアナリシス / リパーゼ活性測定 |
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| Research Abstract |
Quality and freshness assessment of fats and oils can be made based on acidity determination due to the presence of free fatty acids. A commonly used method is alkali titration in alcohol and ether using a phenolphthalein indicator. However, there is the possibility of error in this method due to unclear color change of the indicator. Titration requires much sample and time to conduct. A new, simple, more rapid and reliable method, thus being desirable, is the aim of this study.
We developed a new method for determining free fatty acid content in oils based on the voltammetric reduction prepeak of 2-methyl-1-4-naphthoquinone due to the free fatty acids, based on the proportionality of prepeak height to the acid concentration. The method was extended to the flow injection analysis with electrochemical detection for the free fatty acid determination with high efficiency. This enabled determination of higher fatty acids ranging 25-1500 pmol/test with the relative standard deviation of 1.4 % and the processing efficiency of 60 samples/h.
This method was successfully applied to : (1) Determination of free fatty acid content (acid value) of various samples including plant and fish oils. (2) Determination of lipase and cholinestrase activity. (3) Determinaiton of acid content in foods such as coffe beans.
Based on the above, high-performance liquid chromatography with electrochemical detection was made as a simple and direct means for separating and determining higher fatty acids without tedious pretreatment of sample such as derivatization procedure.
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