| Project/Area Number |
06453106
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物・生体工学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FURUSAKI Shintaro Univ.of Tokyo, Dept.Chem.Biotechnol., Professor, 大学院・工学系研究科, 教授 (40011209)
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| Co-Investigator(Kenkyū-buntansha) |
SEKI Minoru Univ.of Tokyo, Dept.Chem.Biotechnol., Lecturer, 大学院・工学系研究科, 講師 (80206622)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1995: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1994: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Taxol / Paclitaxl / Taxus sp. / Anti-Cancer Drug / Plant Cell Culture / Continuous Production / Immobilization / Bioreactor / 植物培養細胞 / 固定化 / 植物ホルモン / 連続培養 / 生物化学工学 |
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| Research Abstract |
The production characteristics for Taxol (paclitaxl) using free or immobilized cells of the Japanese yew tree, Taxus cuspidata, were investigated. A considerable amount of Taxol was observed in the medium due to secretion by living cells in the suspension culture. Therefore, using a continuous production system with a membrane cell separator or immobilized cells, Taxol productivity was increased by a factor of ten compared to the batch operation. The continuous Taxol production using immobilized cells was successfully performed at about 0.3mg-Taxol/g-DCW/d of specific production rate for up to 40 days. A novel Taxol production process using cultured cells of the yew tree is proposed.
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