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Synthesis of Affinity Gels for Capillary Affinity Electrophoresis of Analysis of Nucleic Acids

Research Project

Project/Area Number 06453150
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 高分子合成
Research InstitutionKagoshima University

Principal Investigator

AKASHI Mitsuru  Engineering, Applied Chemistry and Chemical Engineering, Kagoshima University, Professor, 工学部, 教授 (20145460)

Co-Investigator(Kenkyū-buntansha) KISHIDA Akio  Engineering, Applied Chemistry and Chemical Engineering, Kagoshima University, A, 工学部, 助教授 (60224929)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsCapillary Electrophoresis / Hydrogel / Affinity Ligad / Double Stranded DNA / Intercalator / Vinylmalachite Green / Polyvinyladenine / Capillary Coating / キャピラリー電気泳動 / ポリビニルアルコール / 核酸 / アフィニティー電気泳動 / ポリビニールアデニン / 癌遺伝子 / 配糖体ポリマー
Research Abstract

High-resolution separation of nucleic acids (DNA and RNA) have been widely achieved using gel electrophoresis and HPLC.Recently, capillary gel electrophoresis (CGE) has been developing rapidly, as the resolving power of CGE is much higher than those of gel electrophoresis and HPLC.And the technique of affinity chromatography is also effective for analysis of nucleic acids. In this project, we investigated the capillary affinity gel electrophoresis (CAGE) of nucleic acids as development of an affinity mode of CGE.First, CAGE using polyacrylamide-poly (9-vinyladenine) conjugate gel was very effective for the recognition of thymine in nucleic acids and affinity separation of base-base mismatching DNA from original DNA (20mers) was achieved. Moreover, intercalator such as malachite green was immobilized in water soluble polymers to show the affinity to AT base pairs even in double stranded DNA.Moreover, we studied a new hydrogel for this system and capillary coating using water soluble polymers.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Mitsuru Akashi et al.: "Evaluation of the Selective Binding Ability of Oligodeoxynucle otides to poly(9-vinyladenine)Using Capillary Affinity Gel Electrophoresis" Analytical Sciences. 10. 967-969 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuru Akashi et al.: "Synthesis of Nonionic and Anionic Hydrogels Bearing a Monosaccharide Regidue and Their properties" Journal of Applied Polymer Sciene. 52. 1757-1763 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 明石 満 他: "光スイッチング機能を有するビニルマラカイトグリーンと二本鎖DNAとのその相互作用(2)" polymer preprints,Japan. 44. 2279-2280 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuru Akashi, Akio Kishida et al.: ""Evaluation of the selective binding ability of oligodeoxynucleotides to poly (9-vinyladenine) using capillary affinity gel electrophoresis"" Analytical Science. Vol.10. 967-969 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuru Akashi, Akio Kishida et al.: ""Synthesis of nonionic anionic hydrogels bearing a monosaccharide residue and their properties"" Journal of Applied Polymer Science. Vol.52. 1757-1763 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuru Akashi, Akio Kishida et al.: ""The study of the interaction between vinyl malachite green having photo-switching ability and double stranded DNAs (2)"" Polymer Preprints, Japan. Vol.44. 2279-2280 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mitsuru Akashi et al.: "Evaluation of the Selective Binding Ability of Oligodeoxy nucle otides to poly(9-vinyladenine)Using Capillary Affinity Gel Electrophoresis" Analytical Sciences. 10. 967-769 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mitsuru Akashi et al.: "Synthesis of Nonionic and Anionic Hydrogels Bearing a Monosaccharide Residue and Their Properties" Journal of Applied Polymer Science. 52. 1757-1763 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 明石 満他: "光スイッチング機能を有するビニルマラカイトグリーンと二本鎖DNAとのその相互作用(2)" Polymer preprints,Japan. 44. 2279-2280 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mitsuru Akashi et al: "Evaluation of the Selective Binding Ability of Oligodcoxynucleotides to Poly(9-vinyladenine)Using Capillary Affinity Gel Electrophoresis" Analytical Sciences. 10. 967-769 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mitsuru Akashi et al: "Synthesis of Nonionic and Anionic Hydrogels Bearing a Monosaccharide Residue and Their Properties" Journal of Applied Polymer Science. 52. 1757-1763 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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