| Project/Area Number |
06453161
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MORI Satoshi The University of Tokyo, Agriculture, Professor, 農学部, 教授 (90011915)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Tohru The University of Tokyo, Agriculture Lecturer, 農学部, 助手 (80242163)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Saccharomyces cereviceae / FRE1 / CaMV35S / transgenic tobacco / BPDS / Fe deficiency / PCR / FREI / トランスジエニツク・タバコ / Agrobacterium / Fe^<3+>-reductase / ylast / Nicotiana tabacum / pBI121 / Fe-deficiencuy |
|---|
| Research Abstract |
FRE1 gene was introduced into tobacco. However, the strains which showed significant reductase activity than the control could not be obtained. The length of the mRNA of the introduced gene was observed shorter than the FRE1. This means its transcription starting point was the downstream than the control or the termination of the transcription was upper stream than the control. By introducing several types of point mutagenesis into FRE1 gene, the transgenic plants are again under construction.
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