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Analysis and Protection on Oxidative stress in Vascular Systems

Research Project

Project/Area Number 06453174
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 食品科学・栄養科学
Research InstitutionNagoya University

Principal Investigator

OSAWA Toshihiko (1995)  Nagoya University, Sch.of Agr., Prof., 農学部, 教授 (00115536)

川岸 舜朗 (1994)  名古屋大学, 農学部, 教授 (50023445)

Co-Investigator(Kenkyū-buntansha) UCHIDA Koji  Nagoya Univ., Sch.of Agr., Asc.Prof., 農学部, 助手 (40203533)
HORIO Fumihiko  Nagoya Univ., Sch.of Agr., Asc.Prof., 農学部, 助教授 (20165591)
大澤 俊彦  名古屋大学, 農学部, 助教授 (00115536)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsOxidative stress / Lipid peroxidation / Atherosclerosis / Active oxygen spesies / 4-Hydroxynonenal / Malonaldehyde / Carboxy methyl lysime / 8-Hydroxydeoxyguanosine / アロンアルデヒド / 過酸化脂質 / 4-ヒドロキシ-2-ノネナ-ル(HNE) / HNE修飾タンパク質 / ポリクロナール抗体 / 酸化LDL
Research Abstract

In the living system, oxidative stress is supposed to be induced by active oxygens including lipid-peroxides. In perticular, oxidative stress caused in blood systems is one of the major reason for atherosclerosis and thrombosis. Glutathione-peroxidase, catalase and superxide-dismutase play an important role for antioxidative ptotection from oxidative stress. This year, we examined the effect of lipid peroxidation products, in particular 4-hydroxynonenal on erythrocytes. Glutathione contents in erythrocytes were decreased by the reaction with 4-hydroxynonenal, and it was found that several enzymes which have SH groups such as carbonic anhydrase were found to be damaged. We have also been involved in the involvement of free radicals in diabetes. Recently we found that several biomarkers such as carboxymethyllysine (CML) and 8-hydroxydeoxyguanosine are prexcreted into urine of diabete patients. We also found that novel type of advanced glycation end products are present in blood of diabete patients, which were named as MRX and MRY.MRY and MRY can be produced by the reaction of erythrocytes of rabbit and sheep with glucose, and it was found that arginine and cysteine play an important role as the precursor of MRX and MRY.Chemical analyzes for structure elucidation is now progress.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Katsuzaki,H.: "Sesaminol Glucosides in Sesame Seeds" Phytochemistry. 35. 773-776 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishina,A.: "Antibacterial Substances,3′,4′-Dihydroxyacetophenone,in Coffee Residue." Biosci.Biotech.Biochem.58. 293-296 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraki,M.: "Antioxidative and Antimutagenic Effects of Theaflavins from Black Teas." Mutation Res.323. 29-34 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda,T.: "Antioxidative Pigments Isolated from the Seeds of Phaseolus vulgaris L." J.Agric.Food Chem.42. 248-251 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda,T.: "Supercritical Carbon Dioxide Extraction of Antioxidative Components from Tamarind(Tamarindus indica,L.) Seed Coat." J.Agric.Food Chem.43. 2803-2806 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miura,S.: "Effects of Various Natural Antioxidants on the Cu^<2+>-mediated Oxidative Modification of Low Density Lipoprotein." Biol.Pharm.Bull.18. 1-4 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大澤俊彦: "Generatic Medicine" 抗酸化物による老年病の予防-抗酸化食品, 70 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大澤俊彦: "バイオサイエンスとインダストリー" デザイナ-フ-ズ(植物性食品成分によるがん予防)研究の現状と動向, 75 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katsuzaki, H.: "Sesaminal Glucoside in Sesami Seeds." Phytochemistry. 35. 773-776 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishina, A.: "Antibacterial Substances.3', 4'-Dihydroxyacetophenone, in Coffee Rosidue" Biosci.Biotech.Biochem.58. 293-296 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiraki, M.: "Antioxidative and Antimutagenic Effects of Theaflavins from Blac Teas." Mutation Res.323. 29-34 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda, T.: "Antioxidative Pigments Isolated from the Seeds of Phaseolus vulgaris L." J.Agric.Food.Chem.42. 248-251 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda, T.: "Supercritical Carbon Dioxide Extraction of Antioxidative Components from Tamarind (Tamarindus indica, L) Seed Coat" J.Agric.Food.Chem.43. 2803-2806

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miura, S.: "Effects of Various Natural Antioxidants on the Cu^<2+>-mediated Oxidative Modification of Low Density Lipoprotein." Biol.Pharm Ball. 18. 1-4 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katsuzaki,H.: "Sesaminol Glucosides in Sesame Seeds" Phytochemistry. 35. 773-776 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishina,A.: "Antibacterial Substances,3′,4′-Dihydroxyacetopheonne,in Coffee Residue." Biosci.Biotech.Biochem.58. 293-296 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shiraki,M.: "Antioxidative and Antimutagenic Effects of Theaflavins from Black Teas." Mutation Res.323. 29-34 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuda,T.: "Antioxidative Pigments Isolated from the Seeds of Phaseolus vulgaris L." J.Agric.Food Chem.42. 248-251 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuda,T.: "Supercritical Carbon Dioxide Extraction of Antioxidative Components form Tamarind (Tamarindus indica,L.) seed Coat." J.Agric.Food Chem.43. 2803-2806 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Miura,S.: "Effects of Various Natural Antioxidants on the Cu^<2+>-mediated Oxdative medification of Low Density Lipoprotein" Biol.Pharm.Bull.18. 1-4 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大澤 俊彦: "Generatic Medicine" 抗酸化物による老年病の予防-抗酸化食品, 70 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大澤 俊彦: "バイオサイエンスとインダストリー" デザイナ-フ-ズ(植物性食品成分によるがん予防)研究の現状と動向, 75 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Koji Uchida: "2-Oxo-histidine as a novel biological marker for oxidatively modified proteins." FEBS Letters. 332. 208-210 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Koji Uchida: "Identification of Oxidized Histidine Generated at the Active Site of Cu,Zn-Superoxide Dismutase exposed to H_2O_2-Serective Generation of 2-oxo-histidine at the Histidine 118." J.Biol.Chem.269. 2405-2410 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Koji Uchida: "Michael addition-type 4-hydroxy-2-noneral adducts in modified low density lipoproteins:markers for atherosclerosis." Biochemistry. 33. 12487-12494 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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