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Evaluation of the meaning of foraminiferal test morphology based on DNA sequences

Research Project

Project/Area Number 06454002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Stratigraphy/Paleontology
Research InstitutionShizuoka University

Principal Investigator

KITAZATO Hiroshi  Shizuoka University Institute of Geosciences, Faculty of Science, Professor, 理学部, 教授 (00115445)

Co-Investigator(Kenkyū-buntansha) YOSHINAGA Koichi  Department of Chemistry, Faculty of Science, Professor, 理学部, 教授 (70021954)
CHIBA Satoshi  Institute of Geosciences, Faculty of Science, Research Associate, 理学部, 助手 (10236812)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥4,500,000 (Direct Cost: ¥4,500,000)
KeywordsBenthic Foraminifera / Morphological Evolution / Molecular Evolution / Morphological Analysis / Molecular Biology / DNA / nucleotide sequences / PCR technique
Research Abstract

To evaluate the meaning of benthic foraminiferal test morphology, molecular phylogenetic analyzes have carried out for several selected genera. Glabratella and Bolivina and related genera were selected for analysis, because these genera have been well documented morphologically. Both DNA extractions and PCR techniques were mostly adopted from Pawlowski et al. (1995). We analyzed about 650 nucleotic acid base pairs from LSU ribosomal DNA.Specific primers for foraminifera were used for each PCR step. Amplified DNA fragments were cloned at plasmid vectors, then sequenced with an automatic sequencer.
DNA sequences of forminifera show several interesting results. Genetic variation within local populations of Glabratella is lower than in Bolivina and Ammonia. This may reflect differences in mode of reproduction among foraminiferal genera. Molecular phylogenies calculated from nucleotide sequencing data had different cluster groups than a phylogeny based on morphological data. Calculations of the molecular phylogenetic trees were made with both parsimony and neighbor joining methods. Cluster groups of molecular data were, however, well related to morphological cluster groups that are based on functional morphology. For instance, radial grooves around the aperture area, which may be related to reproductive behavior, is similar among taxa grouped by molecular clustering. In Glabratella opercularis the variation within one of the populations was greater than that fpund within other entire species that we have studied. Whithin this population morphological variation is typical for foraminiferal populations and it is not possible to subdivide the population based on morphological characters. These results suggest that reevaluation of foraminiferal test morphology from the view point of functional morphology may enable creation of phylogenies based on morphology that are cnoformable with molecular phylogenies.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Pawlouski,J.,Bolivar,I., Fahrni.J.,ZanineH :, L and Kitazato,H.: "Partial LSU rDNA sequences of Trochammina spp. (Foraminiferida)" Proc. 4th IWAF,Cracor Pol and Grzybouski Fowdatin Special Publication22GD01:no.3. 227-232 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hemleben C. and H.Kitazato: "Deep Sex foraminifera under long time observation in the loboratory" Deep-Sea Research. V.42. 827-832 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato,H.: "Recolonization by deep-Sea benthic forminifera possikle substrate preterences" Marine Micropaleontology. 26. 65-74 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato,H. and Y. Shirayama: "Rapid creation of a vednced environment and an eavly stage of chemosynthetic community on coffle bores ot the deep sea bottom in Sagami Boy,nirol Japan." Vie et Milien. V.46. 1-5 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato,H. and S. Matsushita: "Laboratory observations of sexnal and asexual reproduction of Trochammira hadar Vclio" Trans. Proc. Palaeontol. Soc. Japan. no 182. 454-466 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sunouchi,M.,F. Sasaki S. Chiba and M.Kawata: "Morphological change and phylogemetic relationships in Tadpole shrimps (Triops)" Biol. Jour. Linnean Soc.(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Pawlowski, J., I.Boliver, J.Fahrni, L.Zaninetti and H.Kitazato: "Partial LSU rDNA sequences of Trochammina spp. (Foraminiferida)." Proc.4th IWAF,Cracow, Poland, Grzybowski Found.Spec.Pub.no.3. 227-232 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato, H.and Ohga, T.: "Seasonal changes in deep-sea benthic foraminiferal population : Results from long term observations at Sagami Bay, Japan." in Sakai, H.and Y.Nozaki, (eds.), Biogeographical Processes and Ocean Flux in the Western Pacific. Terra Scientific Pub., Tokyo. 331-342 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hemleben, Ch. and H.Kitazato: "Deep sea foraminifera under long time observation in the laboratory." Deep-sea Res.v.42. 827-832 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato, H.: "Recolonization by deep-sea benthic foraminifera : possible substrate preferences." Marine Micropaleont. v.26. 65-74 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato, H.and Y.Shirayama: "Rapid creation of a reduced environment and an early stage of chemosynthetic community on cattle bones at the deep-sea bottom in Sagami Bay, Central Japan--An experimental approach to test the stepping stone hypothesis for chemosynthetic community dispersion-." Vie et Milieu. v.46. 1-5 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato, H.and S.Matsushita: "Laboratory observations of sexual and asexual reproduction of Trochammina hadai Uchino." Trans.Proc.Palaeontol.Soc.Japan.N.S.no.182. 454-466 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato, H.: "Benthic foraminifera associated with cold seepages : Discussion of their faunal characteristics and adaptations." FOSSILS. no.60. 48-52 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toyofuku, T., H.Kitazato and H.Kawahata: "Experimental evaluation of the use of Mg/Ca and Sr/Ca ratios of benthic foraminiferal tests as sea water temperature proxies." Proc.Techno-Ocean '96. vol.II. 561-566 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sunouchi, M., F.Sasaki, S.Chiba and M.Kawata: "Morphological change and phylogenetic relationships in Tadpole shrimps (Triops)." Biol. Jour., Linnean Soc. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazato,H and S.Matsushita: "Laboratory observations of sexual and asexual reproduction of Trochammina hadai Vchio" Trans.Proc.Palaeontol.Soc.Japan,N.S.no 182. 454-466 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北里 洋: "冷湧水に伴う底生有孔虫-特徴と適応機構についての考察" 化石. no.60. 48-52 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 豊福高志、北里 洋、川幡穂高: "海水温指標としての底生有孔虫殻Mg/Ca比、Sr/Ca比の実験的評価" テクノオーシャン'96 論文集. II. 561-566 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sunouchi,M,F.Sasaki,S.Chiba and M.Kawata: "Morphelogical change and phylogenetic relationships in Tadpole shrimps (Triops)" Biol.Jour.,Linnean Soc.(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Pawlowski,J. et al.,: "Partial LSU rDNA sequences of Trochammina spp. (Foraminifevida)" Proc. 4th Int. Workshop Agglutinated Foraminifera. 227-232 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kitazato,H. and Shirayama,Y.: "Rapid creation of a veduced enriromnent and an early stages of a chemosynthefic community on a cattle bones of the deep‐sea botton in Sagami Bag, Central Japan" Vie et Milien. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Pawlowski,J.et al.: "Taxonomic vevision of Glabratella spp,based on the sequence of LSU rDNA Diver gont domain D7" Journal of Foraminiferal Research. (発表予定).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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