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Isolation and Structural Analysis of DNA-binding proteins that are specific to chloroplast DNA

Research Project

Project/Area Number 06454012
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理
Research InstitutionTokyo Gakugei University

Principal Investigator

SATO Naoki  Tokyo Gakugei University, Dept.Education, Assoc.Prof., 教育学部, 助教授 (40154075)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥6,000,000 (Direct Cost: ¥6,000,000)
Keywordschloroplast DNA / nucleoid / Envelope membrane / DNA-binding protein / AT-hook / 葉緑体 / サウスウェスタン法 / サウスウエスタン法
Research Abstract

We determined the nucleotide sequence of three cDNAs that encode PD1, PD2, and PD3. These proteins bind specifically to chloroplast DNA.
1.PD1 (348 residues) contained 2 AT-hooks, but it was not homologous to other AT-hook proteins. A membrane-spanning region was also found in PD1. Immunoblot analysis detected bands at 50 kDa and 87 kDa in the chloroplast, as well as a 40 kDa band in the nucleus. Southern blot analysis indicated that PD1 is encoded by a multi gene family.
2.PD2 (632 residues) was a bZIP protein, which contained 6 direct repeats of a sequence with 37 residues. It contained a membrane-spanning domain at its C-terminus. Immunoblot analysis suggested that PD2 was localized in the envelope membrane.
3.PD3 (1629 residues) contained 5 AT-hook motifs and 8 CxxC motifs. Immunoblot analysis suggested that PD3 was localized in both envelope and nucleoid. PD3 was found to be cleaved within the chloroplast to yield two polypeptides.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Sato,N.: "A cold-regulated cyanobacterial gene cluster encodes RNA-binding protein and ribosomal protein S21" Plant Mol.Biol.24. 819-823 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohta,N.,Sato,N.,Kawano,N.,Kuroiwa,T.: "The 'trpA gene on the plastid genome of Cyanidium caldarium RK-1." Curr.Genet.25. 357-361 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato,N.: "A family of cold-regulated RNA-binding protein genes in the cyamobacterium Anabaena variabilis M3." Nucleic Acids Res.23. 2161-2167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato,N.,Misumi,O.,Joyard,J.and Douce R.: "DNA-binding proteins mediate interaction of nucleoids with envelope membrance in developing plastids." Photosynthesis : form Light to Biosphere.Ed.by P.Mathis.3. 635-638 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, N.: "A cold-regulated cyanobacterial gene cluster encodes RNA-binding protein and ribosomal protein S21." Plant Mol.Biol.24. 819-823 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohta, N., N.Sato, S.Kawano and T.Kuroiwa: "The trpA gene on the plastid genome of Cyanidium caldarium strain RK-1." Curr.Genet.25. 357-361 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, N.: "A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena variabilis M3." Nucl.Acids Res.23. 2161-2167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, N., Misumi, O., Joyard, J.and Douce, R.: "DNA-binding proteins mediate interaction of nucleoids with envelope membrane in developing plastids Kluwer Academic Publishers, Dordrecht" In Photosynthesis : from Light to Biosphere. Edited by P.Mathis.Vol.III. 635-638 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato N.: "A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena variabilis M3" Nucleie Acids Research. 23. 2161-2167 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato N.,Misumi O.,Joyard J.and Douce R.: "DNA-binding proteins mediate interaction of nucleoids with envelope membrane in developing plastids." Photosynthesis:from Light to Biosphere(P.Mathis,ed.). III. 635-638 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato,N.: "A cold-regulated cyanobacterial gene cluster encodes RNA-binding protein and ribosomal protein S21" Plant Molecular Biology. 24. 819-824 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohta,N.,Sato,N.,Kawano,S.,Kuroiwa,T.: "The trpA gene on the plastid genome of Cyanidium caldarium strain RK-1" Current Geneics. 25. 357-361 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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