Analysis of induction mechanism of low-temperature-induced transcription factor, LIP19, and identification of its target genes in plant.
| Project/Area Number |
06454016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Akita Prefectural College of Agriculture |
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Principal Investigator |
KUSANO Tomonobu Akita Prefectural College of Agriculture, Biotechnology Institute, Associate Professor, 生物工学研究所, 助教授 (40186383)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | low temperature / gene expression / plant / bZIP protein / transcription factor |
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| Research Abstract |
This year we demonstrated the following results :
(1) A low-temperature-inducible gene (ZmCDPK1) encoding a Ca ion-dependent protein kinase was isolated. Time-course experiment indicates that the low-temperature induction of ZmCDPK1 gene precedes that of mlip15 gene.
(2) Cycloheximide (Chx) at sub-inhibitory concentration of protein synthesis elevates the steady-state levels of ZmCDPK1, mlip15 and Adh1 transcripts. Chalcone synthase-coding gene is induced by low temperature, but not by Chx. Among 8 other drugs including actinomycin D and puromycin, only anisomycin showed identical effect on the expression of above four genes with Chx.
(3) Involvement of Caion in the induction process by low temperature and by Chx is suggested.
(4) Transgenic tobacco plants in which tbz17 gene is constitutively expressed are produced. These plants showed an altered morphology compared with the plants transformed with the control vector.
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Report
(4 results)
Research Products
(7 results)
