| Project/Area Number |
06454035
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物・生体工学
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| Research Institution | TOKYO INSTITUTE OF TECHNOLOGY,RESEARCH LABORATORY OF RESOURCES UTILIZATION |
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Principal Investigator |
SHODA Makoto TOKYO INSTITUTE OF TECHNOLOGY,RESEARCH LABORATORY OF RESOURCES UTILIZATION : PROFESSOR, 資源化学研究所, 教授 (70023489)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Biological treatment / Volatile organic compounds / Fungus / Oxidative enzymes / Activated carbon / Activated carobn fiber / Organic sulfur gases / Peat |
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| Research Abstract |
Screening of microorganisms which have high ability for degradation and deodorization of volatile organic compounds (VOC) was tried intensively and several bacteria and fungi were successfully isolated. Specific analysis for characteristics of fungi was carried out because very few reports have been published on the fungi. In paralle to the microbial analysis, carriers for the effective microorganisms were tested and appropriate one was selected. The following is the summary of the results.
(1) As the result of identification of isolated fungi, they are EXophiala sp. and Geotrichum candidum.
(2) Fungus Exophiala sp. showed a high ability for derading sulfur-containing organic gases especially at lower pH range like 2. The activity was higher in a stationary phase than in logarithmic growth phase.
(3) Some enzymes responsible for degradation of dimethyl sulfide were purified from the cells of Exophiala sp.. Crude enzyme solution showed the optimal pH 7 in a sharp contrast to the result of
… More
the cells. The crede enzyme solution was so unstable that purification of the each enzyme was unsuccessful. The enzymes were found to have SH group at active site.
(4) Fungi Geotrichum candium degraded almost all VOC by producing oxidative enzymes extracellularly.
(5) As carriers for microorganisms, several materials were testes like peat, activated carbon, activated carbon fibers etc. The peat was appropriate in the point that this material contains certain amount of nutrients. However, activated carbon fiber or activated carbon showed oxidation activity for sulfur-containing organic compounds catalytically. Therefore, activated carbon fiber was usefull for combined treatment of the chemical and biological methods. The catalytic mechanism of the activated carbon fiber was fully investigated for sulfur-containing gaese.
(6) The selection of activated carbon was tried among various commercial products with respect to the capacity of cell attachment. The powdered activated carobn was attached on the plastic fiber and the removal of trimethyl amine was conducted by a newly isolated bacteria. Less
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